Pharmacological and ADMET-based pharmacokinetic properties of Syzygium samarangense var. parviflorum leaf extract in in vitro, in vivo and in silico models

Abstract

This research investigated pharmacological properties mainly the anti-inflammatory, anthelmintic, thrombolytic and anxiolytic potential of methanol extract of Syzygium samarangense (MESS) var. parviflorum. Anti-inflammatory action by bovine serum albumin, egg albumin denaturation and membrane stabilization, anthelmintic by live parasites, thrombolytic by clot lysis and anxiolytic by elevated plus maze (EPM) and light and dark box (LDB) tests were measured. The four targeted pharmacological properties were further justified using the most prevalent compounds, isolated from this plant, to be undergone for their pharmacokinetic property’s analyses, sitemap analyses and lignad-receptor interactions by computational models through SwissADME and Schrödinger, 2018 softwares against PDB 6COX, 6D6T, 1JFF receptors. MESS was found to display statistically significant (P < 0.05) inhibition of Bovine Serum albumin and Egg albumin denaturation compared to reference drug diclofenac sodium. Remarkable vermicidal effect on the paralysis and death of anthelmintic parasites was observed at MESS concentration 200 mg/dL. A nondescript clot lysis of MESS compared to streptokinase was evident in in vitro thrombolytic assay. MESS increased the number of times the animal crossed from one compartment to the other and the time spent in the brightly-lit chamber of the LDB. Three-methylchalcone derivatives out of seven MESS compounds were undertaken, based on cut off value and sitemap prediction score, for further ligand-receptor binding efficiency. All these three compounds showed promising docking score, glide emodel and glide energy against PDB 6COX, 6D6T and 1DDJ, plasmin proteins demonstrating the prospects of MESS to be materialized for anti-inflammatory, anthelmintic, and thrombolytic therapeutics with further clarification.

Hydrogel for the Controlled Delivery of Bioactive Components from Extracts of Eupatorium glutinosum Lam. Leaves

“…Aspirin was selected as a positive control as it is a well-known non-steroidal anti-hemolytic agent [ 40 ]. The percentage inhibition of hemolysis in human red blood cell stabilization by aspirin was obtained according to similar assays by Hossain et al [ 41 ]. Specifically, EG-methanolic, the plant’s methanolic extract at 0.05 mg/mL, represented the highest value of inhibition of hemolysis, while at higher concentrations, possibly the synergism of various metabolites decreases, as working with complete extracts may result in all at high concentrations.…”

Green Synthesis and Characterization of Silver Nanoparticles from Nerium indicum and Investigation of Antioxidant and Anti-Cancerous Potential against Cervical Cancer Cell Line (HeLa)

“…Haemolytic activity determination was performed according to the previously used protocol with some modifications. 17 Briefly, 5 mL of the blood was centrifuged, RBC pellet collected after supernatant was discarded and washed twice with PBS. 2 % of the RBC suspension was made in 1% NaCl and 1 mL of this suspension and 1 mL of 25, 50, 100, and 200 µg/mL of the NI AgNPs (dissolved in PBS) were mixed together.…”

Discrimination of Syzygium samarangense cv. ‘Giant Green’ Leaves at Different Maturity Stages by FTIR and GCMS Fingerprinting

“…Traditionally, it is a medicinal plant: various parts are used to treat some health problems such as edema, cracked tongue, asthma, diarrhea, bronchitis, fever, ulcer, sore throat, and to reduce blood pressure [3,4]. Additionally, the Giant Green cultivar contains an abundance of valuable phytochemicals such as phenolic acids, flavonoids, anthocyanins, and carotenes [5], which show antioxidant, antibacterial, antidiabetic, anticancer, and anti-inflammatory activities [6][7][8].…”