Hydrogel for the Controlled Delivery of Bioactive Components from Extracts of Eupatorium glutinosum Lam. Leaves

Abstract

This research reported a hydrogel loaded with the ethanolic and methanolic extracts of Eupatorium glutinosum Lam. The E. glutinosum extracts were characterized by phytochemical screening, Fourier-transform infrared spectroscopy (FTIR), thin-layer chromatography (TLC), and UV/Vis profile identification. This research also evaluated the pharmacological activity of the extracts using antimicrobial, antioxidant, and anti-inflammatory assays prior to polymeric encapsulation. Results indicate that extracts inhibit the Escherichia colii DH5-α (Gram negative) growth; excellent antioxidant activity was evaluated by the ferric reducing power and total antioxidant activity assays, and extracts showed an anti-hemolytic effect. Moreover, the cotton and microcrystalline cellulose hydrogels demonstrate successful encapsulation based on characterization and kinetics studies such as FTIR, extract release, and swelling degree. Moreover, effective antibacterial activity was registered by the loaded hydrogel. The overall results encourage and show that Eupatorium glutinosum-loaded hydrogel may find a wide range of bandage and wound healing applications in the biomedical area.

Use of Plant Extracts, Bee-Derived Products, and Probiotic-Related Applications to Fight Multidrug-Resistant Pathogens in the Post-Antibiotic Era

“…Furthermore, isolated apigenin was effective against P. aeruginosa, K. pneumoniae, S. Typhimurium, P. mirabilis, and K. aerogenes. Similarly, the synergistic antibacterial effect of apigenin, together with beta-lactam antibiotics, was also observed against MRSA [45]. In addition, apigenin and ceftazidime also exhibited a synergistic antibacterial effect against ceftazidime-resistant E. cloacae [103].…”

“…Historically, plant extracts have been used to treat several bacterial infections in medicine [43]. Several techniques have allowed the extraction and identification of bioactive compounds to recognize the mechanism of action that causes bacteriostatic effects [44,45]. Plant extracts which have antibacterial effects can be obtained from roots, fruits, flowers, stems, leaves, and seeds.…”

Antimicrobial activity of stingless bee honey (Tribe: Meliponini) on clinical and foodborne pathogens: A systematic review and meta‐analysis

“…Meanwhile, time‐kill assay is viable technique useful to characterize the antimicrobial activity during time evidencing the dynamic interaction between the optimal concentration of the antimicrobial agent and the microbial pathogens (Balouiri et al., 2016). Therefore, the most suitable approach to characterize the antimicrobial activity of honey samples should be initiated by screening the best candidates by agar disc‐diffusion or overlay assay and a further evaluation of the obtained candidates should also be performed through broth microdilution and time‐kill assays (Cabezas‐Mera et al., 2023; Zamora‐Mendoza et al., 2023).…”

Vaccinium myrtillus L. Leaf Waste as a Source of Biologically Potent Compounds: Optimization of Polyphenol Extractions, Chemical Profile, and Biological Properties of the Extracts

“…The data of this study indicated that bilberry leaf extract compounds maintained the tonicity and balance of the erythrocyte membrane and thereby prevented its lysis. Bioactives of the plant extracts, including tannins, flavonoids, coumarins, etc., can have an important role in decreasing the plasma membrane stress caused by high temperature or hypotonic solution [ 129 ]. Bonarska-Kujawa et al [ 130 ] reported that chlorogenic acid molecules are positioned mainly in the outer part of the erythrocyte membrane (hydrophilic region) and do not induce lysis or osmolality changes, preventing the diffusion of free radicals into the membrane interior.…”