Pharmacokinetics of ampicillin-sulbactam in serum and synovial fluid samples following regional intravenous perfusion in the distal portion of a hind limb of adult cattle

Depenbrock, Sarah M.; Simpson, Katharine M.; Niehaus, Andrew J.; Lakritz, Jeffrey; Papich, Mark G.

doi:10.2460/ajvr.78.12.1372

Cited by 15 publications

(30 citation statements)

References 32 publications

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“…The options for local antimicrobial therapy include intravenous and intraosseous regional limb perfusion (10,11) and sustained release of antimicrobials eluted from implanted carriers such as collagen sponges (12), polymethylmethacrylate (13,14), plaster of Paris (PoP) (7)(8)(9), constant rate infusion systems (15), and hydroxyapatite cement (13). Regional perfusions can achieve antimicrobial concentrations many times higher than the MIC of common pathogens, however use is limited to the distal extremity due to the need to place a tourniquet proximal to the area of interest and because high concentrations of drug are generally not maintained for very long after tourniquet removal.…”

Section: Introductionmentioning

confidence: 99%

“…Antibiotics reported for use in regional perfusions in cattle include florfenicol, ampicillin-sulbactam, marbofloxacin, and ceftiofur (16)(17)(18)(19). Constant rate infusion systems are also able to achieve high antimicrobial concentrations, however breakage or blockage of the intrasynovial catheter is common (15). Non-biodegradable polymethylmethacrylate implants are easy to use, however removal may be necessary.…”

Section: Introductionmentioning

confidence: 99%

“…Non-biodegradable polymethylmethacrylate implants are easy to use, however removal may be necessary. In addition, creating the implant produces an exothermic reaction, thus only heat stable antibiotics may be used (15). Biodegradable implants, including collagen sponges, hydroxyapatite cement, and PoP, offer the advantage of not requiring removal and being potentially more biocompatible.…”

Section: Introductionmentioning

confidence: 99%

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In vitro Elution of Penicillin, Ampicillin, Tetracycline, Tulathromycin, and Florfenicol From Plaster of Paris Beads

et al. 2020

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The objectives of this study were to report a recipe for making antibiotic impregnated Plaster of Paris (AI-PoP) beads using penicillin, ampicillin, tetracycline, tulathromycin, and florfenicol and to determine the in vitro elution rates of those antibiotics in the beads. The AI-PoP beads were made using Plaster of Paris powder, antibiotic, and water, cured for 24 h, sterilized by ethylene oxide, and stored up to 5 months before testing. For each antibiotic, 20 beads were combined with bovine serum in sterile tubes and incubated at 37°C on a rocker. Serum was replaced at intervals over the 14 days study period, and antibiotic concentrations were determined by high pressure liquid chromatography with mass spectrometry. Separately, in a proof-of-concept study, the growth of E. coli and T. pyogenes in eluent from 10 beads for each antibiotic was quantified by flow cytometry. Antibiotic was detected in AI-PoP bead eluent for 14 days for all but the ampicillin beads, for which antibiotic was detected for 8 days. The concentration of antibiotic in eluent was greater than the minimum inhibitory concentration (MIC) of tested bacteria for the entire study period for penicillin, tetracycline, tulathromycin, and florfenicol. The concentration of ampicillin remained greater than the MIC of E. coli for 4 days and T. pyogenes for 6 days. The colony forming units (CFU)/ml of live E. coli and T. pyogenes was reduced over a 72-h period by 1–3 log10 CFU, with the exception of tetracycline, which reduced CFU/ml of T. pyogenes by <log10 CFU. AI-PoP beads containing penicillin, tetracycline, tulathromycin, and florfenicol elute antibiotic well-above the MIC of selected isolates for the 2 weeks study period. These antibiotics show promise for delivery in joint and wound infections.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro Elution of Penicillin, Ampicillin, Tetracycline, Tulathromycin, and Florfenicol From Plaster of Paris Beads

et al. 2020

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“…IVRLP with antimicrobials was first reported in cattle in 1974, 5 and several clinical studies investigated its use for the treatment of digital septic lesions. 6 The pharmacokinetics of cefazolin, 7 ceftiofur, 8 florfenicol, 9 tetracycline hydrochloride 3 and ampicillin-sulbactam 10 after IVRLP were defined previously in cattle.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics of marbofloxacin administered via intravenous regional limb perfusion in dairy cows: evaluation of two different tourniquets

Celani

Tulini

Montesano

et al. 2017

Veterinary Record Open

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ObjectivesThis study evaluated synovial and systemic plasma pharmacokinetic variables of marbofloxacin after a single intravenous regional limb perfusion (IVRLP) performed using two different tourniquets in clinically healthy, standing, non-sedated dairy cows. The authors hypothesised that the type of tourniquet used for IVRLP would influence the synovial fluid concentration of marbofloxacin in the perfused distal limb.DesignThe study had a randomised parallel-group design.MethodsTen adult dairy cows were included. Unilateral hindlimb IVRLP through the dorsal common digital III vein was performed in two groups of five cows (group 1: wide rubber tourniquet; group 2: manual pneumatic tourniquet) using 0.67 mg/kg of marbofloxacin. The tourniquet was applied proximal to the tarsus and maintained for 30 minutes. Samples of jugular blood and synovial fluid from the tibiotarsal joints of the perfused limb were obtained before and at intervals after IVRLP. All samples were analysed for drug concentrations using liquid chromatography tandem mass spectrometry. Pharmacokinetic parameters were determined to establish the influence of tourniquet types. Differences were considered significant at P≤0.05.ResultsNo adverse effects from the procedure or marbofloxacin were observed in any animal. Significant differences in synovial concentrations and pharmacokinetic parameters were measured. The mean ± sd areas under the concentration versus time curve from time 0 to 24 hours were 178.98±58.08 µg hour/ml for group 2 and 21.11±9.93 µg hour/ml for group 1. The mean ± sd maximum marbofloxacin concentrations were 75.50±10.19 µg/ml for group 2 and 6.35±1.47 µg/ml for group 1.ConclusionsPerforming IVRLP using the dorsal common digital III vein and a manual pneumatic tourniquet set at 300 mmHg above the tarsus in standing cows resulted in significantly higher marbofloxacin concentrations in the tibiotarsal joint compared with those with the wide rubber tourniquet.Trial registrationLocal ethical committee (number 41/2012/CEISA).

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“…Já uso de antibiótico regional é relatado por 24% dos respondentes. O uso de antibióticos regionais possui a vantagem de apresentar alta concentração local, mas baixa concentração sistêmica e por curto período DEPENBROCK et al, 2017) A imunidade adequada dos animais se mostra um importante fator para a prevenção de doenças. No estudo realizado por , os veterinários acreditavam que a vacinação do rebanho e a transferência de imunidade passiva estão entre as práticas que podem reduzir o uso de antibióticos.…”

Section: Aspectos Do Uso De Aciunclassified

>b<Percepção dos buiatras sobre o impacto do emprego de antibióticos em bovinos leiteiros no Estado de São Paulo>/b<

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Agradeço, primeiramente, ao Sergio-meu marido, amor, parceiro e melhor amigo. Aqui não caberiam, nunca, todos os motivos, pois são todos! Obrigada por estar ao meu lado em cada segundo. Agradeço aos meus pais por serem as melhores pessoas que poderiam ser e por me ensinarem a tentar ser a melhor pessoa possível também. Por todo o amor. Agradeço aos meus irmãos Léo e Pedro, que em suas particularidades, me ensinam o que é amor incondicional fraterno. À minha família como um todo, mas em especial minha prima Raiane e Tia Bete, filha e mãe, muito queridas; e à minha vó Regina, querida, que nos deixou esse ano para morar com as estrelas. À Profa. Alice Della Libera, orientadora para a ciência e para assuntos da vida. Agradeço pela sensibilidade e por me permitir estar aqui nesse momento. Agradeço pelas portas que me abriu e por ensinar a abrir novas portas sozinha. À Aline, amada amiga, por todos os momentos que nos ensinam a fortalecer uma à outra, e que fazem nossa amizade crescer e ficar mais bonita. Por ser determinada e corajosa, com um coração maior que você mesma. Ao José, amado amigo, que sempre me salva com o sorriso no rosto e carinho no coração. Companhia que deixa a gente leve e feliz, e melhora nosso dia instantaneamente! Obrigada por ensinar a ter leveza na vida, sem perder a seriedade do compromisso.

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Pharmacokinetics of ampicillin-sulbactam in serum and synovial fluid samples following regional intravenous perfusion in the distal portion of a hind limb of adult cattle

Cited by 15 publications

References 32 publications

In vitro Elution of Penicillin, Ampicillin, Tetracycline, Tulathromycin, and Florfenicol From Plaster of Paris Beads

In vitro Elution of Penicillin, Ampicillin, Tetracycline, Tulathromycin, and Florfenicol From Plaster of Paris Beads

Pharmacokinetics of marbofloxacin administered via intravenous regional limb perfusion in dairy cows: evaluation of two different tourniquets

>b<Percepção dos buiatras sobre o impacto do emprego de antibióticos em bovinos leiteiros no Estado de São Paulo>/b<

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