Pharmacokinetic study of metoprolol in rabbit plasma by capillary electrophoresis with laser-induced Fluorescence detection

Abstract

This work described a sensitive method for determination of metoprolol in rabbit plasma. The method involved purification by ultrafiltration, derivatization with Fluorescein isothiocyanate, separation by capillary electrophoresis and determination by laser induced fluorescence detector. Other components in plasma including a variety of amino acids and proteins did not interfere with the determination of metoprolol under experimental conditions. The assay had a wide range (2.0-500 ng/mL) of linearity and a detection limit of 0.8 ng/mL. The intra and inter day precisions of the QC samples were satisfactory with RSD less than 10% and accuracy within 10%. This method was successfully applied to pharmacokinetic study of meto prolol in rabbit blood.

Hyphenation of field‐amplified sample injection and transient isotachophoresis in CE for the determination of sotalol and metoprolol in human urine samples

“…The results of the analysis were compared with those from other methods. The LODs and LOQs of Sot and Met for standard samples (or urine samples) were lower than those obtained via colorimetry [4], spectrophotometry [5], ECD [6,7], HPLC‐photodiode array UV [14], HPLC‐ECL [15], CE‐UV [16], CE‐ECD [17], and CE‐ECL [18], but higher than those obtained via GC‐MS [8], HPLC‐MS/MS [9,10] HPLC‐fluorescence [12], HPLC‐UV [13], and CE‐LIF [19]. Wide linear ranges were obtained using most methods.…”

“…These include colorimetry [4], spectrophotometry [5], electrochemical detection (ECD) [6,7], GC‐MS [8], HPLC methods with MS/MS [9–11], fluorescence [12], UV [13,14], and electrochemiluminescence (ECL) detection [15]. Additionally, CE‐UV [16], CE‐ECD [17], CE‐ECL [18], and CE‐LIF detection [19] has been established.…”

Spectrophotometric and Multivariate Calibration Techniques for Simultaneous Determination of Different Drugs in Pharmaceutical Formulations and Human Urine: Evaluation of Greenness Profile

“…AT undergoes little or no metabolism in the liver and is mainly excreted in urine (over 85% of the absorbed drug is excreted in urine as unchanged drug) [1]. Different analytical techniques have been reported for the analysis of AT such as high performance liquid chromatography (HPLC) [2][3][4], spectrophotometric [2,5,6], capillary electrophoresis [7,8], gas chromatography (GC) [9], and spectrofluorimetric [10] methods. Paracetamol (PR) or acetaminophen (Figure 1(b)) is an over-the-counter (OTC) analgesic and antipyretic drug.…”