Abstract:In order to increase stabilities and controlled/sustained released of T4 phages were encapsulated within alginate beads which were then coated with chitosan, polyethylene imine (PEI). Quite high loading capacities (over 90%) were achieved in these pH-sensitive microbeads. Coating with those polycations increased significantly stability both in "simulated gastric fluid" and bile salts especially in the case of PEI coating. The tests conducted in "simulated intestinal fluid" demonstrated that phages were release… Show more
“…Sodium alginate-based matrices have been found suitable to incorporate different phages (Colom et al, 2017;Moghtader et al, 2017). In this work, similar titres of entrapped phages (Table 2) were obtained as compared to a previous work, which attests the efficiency of the entrapment strategy (Alves et al, 2018).…”
Section: Incorporation Of Phages And/or Cnma On Sodium-alginate Filmssupporting
confidence: 80%
“…Most of encapsulation strategies reported for phages were driven by the need to protect them from the adverse conditions found in the digestive tract, such as the low pH and the activity of enzymes. In this context, phages have been encapsulated mostly in alginate-based microspheres (Abdelsattar, Abdelrahman, Dawoud, Connerton, & El-Shibiny, 2019;Colom et al, 2017;Moghtader, Eğri, & Piskin, 2017) and liposomes (Otero et al, 2019). More recently, studies have reported phages encapsulation to be further applied in food products.…”
Notwithstanding the implementation of good processing practices in food companies and appropriate washing of food products by the consumer, Salmonella and Escherichia coli outbreaks continue to occur. In this study, different combinations of bacteriophages (phages) and cinnamaldehyde (CNMA) were incorporated on sodium alginate emulsion-based films to impart them with antimicrobial activity towards S. Enteritidis and E. coli. Films were prepared by casting and they were characterized in terms of CNMA and/or phages loading, thickness, moisture content, water vapor permeability (WVP), swelling index (SW), chemical interactions by FTIR, surface morphology by SEM and antimicrobial performance. Results showed that phages incorporation was not compromised by CNMA as evidenced by their viability inside the films. Increasing CNMA concentration yielded formulations less heterogeneous and a higher amount of CNMA loaded. Films characterization revealed that, in general, phages incorporation did not introduce significant changes on films parameters while the presence of CNMA increased the roughness, thickness and swelling ability of films. Sodium alginate films incorporated with EC4 and φ135 phages displayed antimicrobial activity against E. coli and S. Enteritidis, respectively, while CNMA empowered the films with activity against both species. Combination of both phages with the higher concentration of CNMA resulted in a synergic antimicrobial effect against E. coli and a facilitative effect against Salmonella. Overall, incorporation of EC4 and φ135 phages together with CNMA on alginate emulsion-based films holds great potential to be further applied in food packaging to prevent food contamination.
“…Sodium alginate-based matrices have been found suitable to incorporate different phages (Colom et al, 2017;Moghtader et al, 2017). In this work, similar titres of entrapped phages (Table 2) were obtained as compared to a previous work, which attests the efficiency of the entrapment strategy (Alves et al, 2018).…”
Section: Incorporation Of Phages And/or Cnma On Sodium-alginate Filmssupporting
confidence: 80%
“…Most of encapsulation strategies reported for phages were driven by the need to protect them from the adverse conditions found in the digestive tract, such as the low pH and the activity of enzymes. In this context, phages have been encapsulated mostly in alginate-based microspheres (Abdelsattar, Abdelrahman, Dawoud, Connerton, & El-Shibiny, 2019;Colom et al, 2017;Moghtader, Eğri, & Piskin, 2017) and liposomes (Otero et al, 2019). More recently, studies have reported phages encapsulation to be further applied in food products.…”
Notwithstanding the implementation of good processing practices in food companies and appropriate washing of food products by the consumer, Salmonella and Escherichia coli outbreaks continue to occur. In this study, different combinations of bacteriophages (phages) and cinnamaldehyde (CNMA) were incorporated on sodium alginate emulsion-based films to impart them with antimicrobial activity towards S. Enteritidis and E. coli. Films were prepared by casting and they were characterized in terms of CNMA and/or phages loading, thickness, moisture content, water vapor permeability (WVP), swelling index (SW), chemical interactions by FTIR, surface morphology by SEM and antimicrobial performance. Results showed that phages incorporation was not compromised by CNMA as evidenced by their viability inside the films. Increasing CNMA concentration yielded formulations less heterogeneous and a higher amount of CNMA loaded. Films characterization revealed that, in general, phages incorporation did not introduce significant changes on films parameters while the presence of CNMA increased the roughness, thickness and swelling ability of films. Sodium alginate films incorporated with EC4 and φ135 phages displayed antimicrobial activity against E. coli and S. Enteritidis, respectively, while CNMA empowered the films with activity against both species. Combination of both phages with the higher concentration of CNMA resulted in a synergic antimicrobial effect against E. coli and a facilitative effect against Salmonella. Overall, incorporation of EC4 and φ135 phages together with CNMA on alginate emulsion-based films holds great potential to be further applied in food packaging to prevent food contamination.
“…as ion-exchange reaction would be taken place when the beads absorb bile salt (Li et al, 2011;Moghtader et al, 2017). Thus, this enhanced the mass transfer resistance and limits the penetration of bile salt into the beads (Obradović et al, 2015).…”
Aims: Microencapsulation has been used to protect the viability of probiotics in harsh environments such as gastrointestinal conditions and food composition. The present study aimed to optimize the microencapsulation of Lactobacillus plantarum 299v (Lp299v) using co-extrusion by varying two parameters (calcium chloride (CaCl2) and oligofructose (FOS) concentrations) and storage stability of the beads produced in ambarella juice at refrigerated and room temperature. Methodology and results: Chitosan coated-alginate microcapsule prepared with 4.0% (w/v) FOS and 2.5% (w/v) CaCl2 showed highest microencapsulation efficiency (93%). The microcapsules were subjected to gastrointestinal treatment and storage test in ambarella juice. Both encapsulated Lp299v with and without FOS showed higher viabilities compared with free cells after incubated in simulated gastric juice (SGJ) and simulated intestinal juice (SIJ). After 5 h of incubation in SIJ, the viabilities of both encapsulated probiotic with and without FOS were more than 10 7 CFU/mL. The Lp299v were stored in ambarella juice under refrigerated (4 °C) and room temperature (25 °C) for 4 weeks. At 25 °C, all forms of Lp299v lost their viabilities after one week. On the other hand, at 4 °C, viable cells count of both encapsulated Lp299v with and without FOS were reported to be more than 10 7 CFU/mL after 4 weeks of storage. Conclusion, significance and impact of study: Microencapsulation with FOS was able to improve Lp299v's viability during storage in low pH fruit juices compared to those without FOS. The microencapsulated probiotics could be applied in ambarella juice for the development of functional food.
“…As was shown by Międzybrodzki et al, only a small fraction (<0.1%) of oral administered phage was observed in the intestines, even after neutralizing antacid administration. The rationale behind several reviewed studies is to overcome such problems by protecting bacteriophages against harmful environments through embedding in a biomaterial matrix (Kim et al, 2015;Moghtader et al, 2017;Abdelsattar et al, 2019;Otero et al, 2019;Vinner et al, 2019).…”
Section: Advantages Of Bacteriophage Loaded Biomaterials For Local Dementioning
As viruses with high specificity for their bacterial hosts, bacteriophages (phages) are an attractive means to eradicate bacteria, and their potential has been recognized by a broad range of industries. Against a background of increasing rates of antibiotic resistance in pathogenic bacteria, bacteriophages have received much attention as a possible "last-resort" strategy to treat infections. The use of bacteriophages in human patients is limited by their sensitivity to acidic pH, enzymatic attack and short serum half-life. Loading phage within a biomaterial can shield the incorporated phage against many of these harmful environmental factors, and in addition, provide controlled release for prolonged therapeutic activity. In this review, we assess the different classes of biomaterials (i.e., biopolymers, synthetic polymers, and ceramics) that have been used for phage delivery and describe the processing methodologies that are compatible with phage embedding or encapsulation. We also elaborate on the clinical or preclinical data generated using these materials. While a primary focus is placed on the application of phage-loaded materials for treatment of infection, we also include studies from other translatable fields such as food preservation and animal husbandry. Finally, we summarize trends in the literature and identify current barriers that currently prevent clinical application of phage-loaded biomaterials.
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