Microencapsulation of Lactobacillus plantarum 299v incorporated with oligofructose in chitosan coated-alginate beads and its storage stability in ambarella juice

Abstract

Aims: Microencapsulation has been used to protect the viability of probiotics in harsh environments such as gastrointestinal conditions and food composition. The present study aimed to optimize the microencapsulation of Lactobacillus plantarum 299v (Lp299v) using co-extrusion by varying two parameters (calcium chloride (CaCl2) and oligofructose (FOS) concentrations) and storage stability of the beads produced in ambarella juice at refrigerated and room temperature. Methodology and results: Chitosan coated-alginate microcapsule prepared with 4.0% (w/v) FOS and 2.5% (w/v) CaCl2 showed highest microencapsulation efficiency (93%). The microcapsules were subjected to gastrointestinal treatment and storage test in ambarella juice. Both encapsulated Lp299v with and without FOS showed higher viabilities compared with free cells after incubated in simulated gastric juice (SGJ) and simulated intestinal juice (SIJ). After 5 h of incubation in SIJ, the viabilities of both encapsulated probiotic with and without FOS were more than 10 7 CFU/mL. The Lp299v were stored in ambarella juice under refrigerated (4 °C) and room temperature (25 °C) for 4 weeks. At 25 °C, all forms of Lp299v lost their viabilities after one week. On the other hand, at 4 °C, viable cells count of both encapsulated Lp299v with and without FOS were reported to be more than 10 7 CFU/mL after 4 weeks of storage. Conclusion, significance and impact of study: Microencapsulation with FOS was able to improve Lp299v's viability during storage in low pH fruit juices compared to those without FOS. The microencapsulated probiotics could be applied in ambarella juice for the development of functional food.

Application of biomacromolecules encapsulation systems for the long-term storage of Lactobacillus plantarum F1 and Lactobacillus reuteri 182

“…In A-T-P compositions, the encapsulation efficiency via extrusion technique decreased for approximately 2 % (97.95 ± 0.41 % for L. plantarum and 97.98 ± 0.55 % for L. reuteri ). This is in agreement with Gandomi et al Kim et al, Ng et al and Chan and Pui, who obtained lower encapsulation yield with the addition of prebiotics [ [22] , [23] , [24] , [25] ]. It might be explained by the capsules inner space being taken up by the prebiotics, thus resulting in lower number of the entrapped cells in the capsules as suggested by Ng et al [ 24 ].…”

“…This is in agreement with Gandomi et al Kim et al, Ng et al and Chan and Pui, who obtained lower encapsulation yield with the addition of prebiotics [ [22] , [23] , [24] , [25] ]. It might be explained by the capsules inner space being taken up by the prebiotics, thus resulting in lower number of the entrapped cells in the capsules as suggested by Ng et al [ 24 ]. The survival rates of the LAB cells in the A-T-P capsules were similar to the A-T capsules.…”

Effect of added prebiotic (Isomalto-oligosaccharide) and Coating of Beads on the Survival of Microencapsulated Lactobacillus rhamnosus GG

“…These characteristics are vital for mass production. The probiotic microcapsule incorporated with OMD also exhibited a high MEE which is suitable for further application in food industry such as yogurt, cheese, fruit juice, herbal tea and cereal (Champagne et al, 2018;Heydari et al, 2018;Murtaza et al, 2017;Ng et al, 2019;Wong et al, 2019). Figure 3 shows the viability of free cells and microencapsulated LGG under sequential digestion.…”

Microencapsulation of Lactiplantibacillus plantarum with inulin and evaluation of survival in simulated gastrointestinal conditions and roselle juice

“…The preparation of chitosan aqueous solution was adapted from Ng et al (2019). To prepare the chitosan solution (0.1% w/v), 1 g of chitosan powder was dissolved in 900 mL of distilled water acidified with 10 mL of glacial acetic acid.…”

“…Wall materials such as alginate have been incorporated during the microencapsulation process in order to protect the core material. Alginate is a linear anionic polysaccharide derived from different types of algae and it is commonly used as shell wall material (Ng et al, 2019). It is made up of two structural units which are the L-guluronic acid and D-mannuronic acid that are joined through the glycosidic bond (Heise et al, 2005).…”