Phage-displayed nanobody based double antibody sandwich chemiluminescent immunoassay for the detection of Cry2A toxin in cereals

Qiu, Yulou; Li, Pan; Liu, Beibei; Liu, Yuan; Wang, Yulong; Tao, Tingting; Xu, Junli; Hammock, Bruce D.; Liu, Xianjin; Guan, Rongfa; Zhang, Cunzheng

doi:10.1080/09540105.2019.1642307

Cited by 13 publications

(5 citation statements)

References 41 publications

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“…The enzyme-labeled speci c antibody Ab2-E is added to react with the antigen. Finally, the substrate solution is added, resulting in chemiluminescence detection [29]. Competing binding between the labeled antigen and the unlabeled antigen to be examined and the antibody underlies the competitive inhibition approach, also known as the competition method.…”

Section: Related Workmentioning

confidence: 99%

RETRACTED ARTICLE: Analysis of relationship between tumor markers and detection of tumors by chemiluminescence immunoassay and artificial neural networks

2023

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The health of people is seriously threatened by the class of disorders known as tumors because of their high risk and high mortality. Early detection and treatment are the most e cient approaches to stop cancers and lower mortality, according to a substantial body of research and preventative data.Therefore, the search for early-diagnosis tumor indicators has taken center stage. The existence of tumors may be detected by a family of molecules known as tumor markers, which have a high sensitivity and speci city. Tumor markers are mainly used for diagnosing tumors, judging e cacy and evaluating prognosis. At present, the detection methods of tumor markers include enzyme-labeled immunoassay technology and chemiluminescence immunoassay (CLIA) technology. In recent years, with the continuous development of in vitro diagnostic technologies, CLIA has the advantages of high sensitivity, wide detection range, simple operation, good repeatability and speci city, high degree of automation, and no radioactive staining of reagents. It has a good application prospect in clinical medical diagnosis. In this paper, the relationship between tumor markers and tumors is detected by CLIA, and then the tumor markers are analyzed by arti cial neural network, and the classi cation and screening of cancer detection is completed. Finally completed the following work: 1) Introduced the research status of CLIA at home and abroad, and provided a theoretical basis for the analysis method proposed later. 2) The technical principle of ANN is introduced and the SAE neural network model is proposed. 3) Select the model evaluation index, and select the SAE parameters through experiments to construct the optimal SAE model. Input the sample data and then get the accuracy, recall and F1 score of the model. Compared with other models, it can be found that the SAE model proposed in this paper has the best detection performance.

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Section: Related Workmentioning

confidence: 99%

RETRACTED ARTICLE: Analysis of relationship between tumor markers and detection of tumors by chemiluminescence immunoassay and artificial neural networks

2023

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“…QBs were purchased from Kundao (Shanghai, China). Phage-displayed nanobody P2 and anti-Cry2A PAb were prepared previously [ 40 ]. HRP-labeled goat anti-rabbit antibody was purchased from Solarbio (Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous work, several phage-displayed nanobodies against the Cry2A toxin were isolated from a naive nanobody library [ 40 ]. In this study, the anti-Cry2A nanobody P2 was expressed and purified as the capture antibody.…”

Section: Introductionmentioning

confidence: 99%

Quantum-Dot-Bead-Based Fluorescence-Linked Immunosorbent Assay for Sensitive Detection of Cry2A Toxin in Cereals Using Nanobodies

Qiu

You

et al. 2022

Foods

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In this study, a quantum-dot-bead (QB)-based fluorescence-linked immunosorbent assay (FLISA) using nanobodies was established for sensitive determination of the Cry2A toxin in cereal. QBs were used as the fluorescent probe and conjugated with a Cry2A polyclonal antibody. An anti-Cry2A nanobody P2 was expressed and used as the capture antibody. The results revealed that the low detection limit of the developed QB-FLISA was 0.41 ng/mL, which had a 19-times higher sensitivity than the traditional colorimetric ELISA. The proposed assay exhibited a high specificity for the Cry2A toxin, and it had no evident cross-reactions with other Cry toxins. The recoveries of Cry2A from the spiked cereal sample ranged from 86.6–117.3%, with a coefficient of variation lower than 9%. Moreover, sample analysis results of the QB-FLISA and commercial ELISA kit correlated well with each other. These results indicated that the developed QB-FLISA provides a potential approach for the sensitive determination of the Cry2A toxin in cereals.

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“…Later, Qiu et al fabricated a novel phage-displayed nanobody-based DAS-CLIA to detect Cry2A toxin, demonstrated in Figure S2b of the Supporting Information; the assay depicts extensive linear regression from 0.1 to 1000 ng/mL and LOD of 0.09 ng/mL. The assay was validated as the recoveries ranged between 82.7 and 118% and CV of <10% in cereal samples . These chemiluminescent assays provide convenient, efficient, and precise quantification of the signal, giving comparable LODs.…”

Section: Elisamentioning

confidence: 99%

“…The assay was validated as the recoveries ranged between 82.7 and 118% and CV of <10% in cereal samples. 56 These chemiluminescent assays provide convenient, efficient, and precise quantification of the signal, giving comparable LODs.…”

Section: Elisamentioning

confidence: 99%

Advances in the Immunoassays for Detection of Bacillus thuringiensis Crystalline Toxins

Faheem

Qin

Nan

et al. 2021

J. Agric. Food Chem.

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Insect-resistant genetically modified organisms have been globally commercialized for the last 2 decades. Among them, transgenic crops based on Bacillus thuringiensis crystalline (Cry) toxins are extensively used for commercial agricultural applications. However, less emphasis is laid on quantifying Cry toxins because there might be unforeseen health and environmental concerns. Immunoassays, being the preferred method for detection of Cry toxins, are reviewed in this study. Owing to limitations of traditional colorimetric enzyme-linked immunosorbent assay, the trend of detection strategies shifts to modified immunoassays based on nanomaterials, which provide ultrasensitive detection capacity. This review assessed and compared the properties of the recent advances in immunoassays, including colorimetric, fluorescence, chemiluminescence, surface-enhanced Raman scattering, surface plasmon resonance, and electrochemical approaches. Thus, the ultimate aim of this study is to identify research gaps and infer future prospects of current approaches for the development of novel immunosensors to monitor Cry toxins in food and the environment.

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Phage-displayed nanobody based double antibody sandwich chemiluminescent immunoassay for the detection of Cry2A toxin in cereals

Cited by 13 publications

References 41 publications

RETRACTED ARTICLE: Analysis of relationship between tumor markers and detection of tumors by chemiluminescence immunoassay and artificial neural networks

RETRACTED ARTICLE: Analysis of relationship between tumor markers and detection of tumors by chemiluminescence immunoassay and artificial neural networks

Quantum-Dot-Bead-Based Fluorescence-Linked Immunosorbent Assay for Sensitive Detection of Cry2A Toxin in Cereals Using Nanobodies

Advances in the Immunoassays for Detection of Bacillus thuringiensis Crystalline Toxins

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