Phaeobacter daeponensis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea

Abstract

A Gram-negative, motile and egg-shaped bacterium, strain TF-218 T , was isolated from a tidal flat at Daepo Beach (Yellow Sea), Korea, and subjected to a polyphasic taxonomic analysis. Strain TF-218 T grew optimally at pH 7.0-8.0 and 37 6C in the presence of 2 % (w/v) NaCl. It contained Q-10 as the predominant ubiquinone and C 18 : 1 v7c and 11-methyl C 18 : 1 v7c as the major fatty acids. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two unidentified lipids and an aminolipid. The DNA G+C content was 64.9 mol%. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain TF-218 T is phylogenetically closely related to the genera Phaeobacter, Leisingera and Marinovum of the Alphaproteobacteria. The phylogenetic and chemotaxonomic similarities suggest that strain TF-218 T represents a member of the genus Phaeobacter. DNA-DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain TF-218 T differs from the recognized Phaeobacter species. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain TF-218 T represents a novel species of the genus Phaeobacter, for which the name Phaeobacter daeponensis sp. nov. is proposed. The type strain is TF-218 T (=KCTC 12794 T =JCM 13606 T ).

Leisingera aquimarina sp. nov., isolated from a marine electroactive biofilm, and emended descriptions of Leisingera methylohalidivorans Schaefer et al. 2002, Phaeobacter daeponensis Yoon et al. 2007 and Phaeobacter inhibens Martens et al. 2006

“…The remaining fatty acids constituted minor fractions only (,1.0 %). The predominant fatty acids observed were very similar to those of Phaeobacter daeponensis, Phaeobacter gallaeciensis, Phaeobacter inhibens and L. methylohalidivorans (Yoon et al, 2007), indicating that strain LMG 24366 T was closely related to Phaeobacter species and to L. methylohalidivorans.…”

“…Members of the genus Phaeobacter (P. daeponensis LMG 24139 T , P. inhibens LMG 22475 T and P. gallaeciensis LMG 23163 T ) (Martens et al, 2006;Yoon et al, 2007) were also closely related to strain LMG 24366 T , with 16S rRNA gene sequence similarities of 96.9-97.2 %.…”

Seohaeicola saemankumensis gen. nov., sp. nov., isolated from a tidal flat

“…The genus Phaeobacter was created by Martens et al (2006) through the reclassification of Roseobacter gallaeciensis and the description of one novel species, Phaeobacter inhibens. Two further species of the genus have been described recently, Phaeobacter daeponensis (Yoon et al, 2007) and Phaeobacter caeruleus (Vandecandelaere et al, 2009). The genus Marinovum was created by the reclassification of Ruegeria algicola (previously Roseobacter algicola) (Martens et al, 2006).…”

“…Strain SD-15 T differed from the genera Phaeobacter, Leisingera and Marinovum in that aminolipid was absent (Table 1). Strain SD-15 T could be differentiated from the three phylogenetically related (Schaefer et al, 2002;Martens et al, 2006;Yoon et al, 2007); 4, Marinovum (Lafay et al, 1995;Ruiz-Ponte et al, 1998;Martens et al, 2006;Yoon et al, 2007). +, Positive; 2, negative; W, weakly positive; ND, not determined or not described; V, variable reaction; PC, phosphatidylcholine; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; PL, unidentified phospholipid; AL, aminolipid; L, unidentified lipid.…”

Sediminimonas qiaohouensis gen. nov., sp. nov., a member of the Roseobacter clade in the order Rhodobacterales

“…The novel strains could be distinguished from these bacteria on the basis of differences in the proportions of several fatty acids, including C 16 : 0 , C 18 : 1 v9c, C 10 : 0 3-OH, C 12 : 1 3-OH and C 19 : 0 cyclo v8c (Table 1). (Petursdottir & Kristjansson, 1997;Yi et al, 2007); 4, Phaeobacter daeponensis TF-218 T (Yoon et al, 2007); 5, Yangia pacifica DX5-10 T (Dai et al, 2006); 6, Roseovarius tolerans EL-172 T (Labrenz et al, 1999); 7, Sagittula stellata ATCC 700073 T (González et al, 1997); 8, Roseivivax halodurans JCM 10272 T ; 9, Roseivivax halotolerans JCM 10271 T (Suzuki et al, 1999;Nishimura et al, 1994) Genomic DNA extraction, PCR amplification of 16S rRNA gene and sequencing of the purified PCR products were performed as described previously (Cui et al, 2001). The almost-complete 16S rRNA gene sequences of strains YIM B024 T (1368 bp) and YIM B025 (1357 bp) were obtained and compared with those available in GenBank using BLAST (Altschul et al, 1990).…”