Leisingera aquimarina sp. nov., isolated from a marine electroactive biofilm, and emended descriptions of Leisingera methylohalidivorans Schaefer et al. 2002, Phaeobacter daeponensis Yoon et al. 2007 and Phaeobacter inhibens Martens et al. 2006

Vandecandelaere, Ilse; Segaert, Eveline; Mollica, A.; Faimali, Marco; Vandamme, Peter

doi:10.1099/ijs.0.65844-0

Cited by 44 publications

(28 citation statements)

References 22 publications

(16 reference statements)

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“…Strains: 1, Phaeobacter arcticus DSM 23566 T (Zhang et al, 2008; Vandecandelaere et al, 2009; Freese et al, 2013; Liu et al, 2014); 2, Phaeobacter leonis 306 T (Gaboyer et al, 2013); 3, Phaeobacter gallaeciensis CIP 105210 T (Ruiz-Ponte et al, 1998; Martens et al, 2006; Yoon et al, 2007; Vandecandelaere et al, 2008, 2009; Lucena et al, 2012; Frank et al, 2014; Liu et al, 2014); 4, Phaeobacter (Ruiz-Ponte et al, 1998; Martens et al, 2006; Yoon et al, 2007; Vandecandelaere et al, 2008, 2009; Lucena et al, 2012; Frank et al, 2014; Liu et al, 2014); 4, Phaeobacter inhibens T5 T (Martens et al, 2006; Yoon et al, 2007; Vandecandelaere et al, 2008, 2009; Lucena et al, 2012; Dogs et al, 2013b); 5, Leisingera aquimarina DSM 24565 T (Vandecandelaere et al, 2008, 2009; Sun et al, 2010; Riedel et al, 2013); 6, Leisingera methylohalidivorans MB2 T (Schaefer et al, 2002; Martens et al, 2006; Yoon et al, 2007; Vandecandelaere et al, 2008; Sun et al, 2010; Jin et al, 2011; Buddruhs et al, 2013a); 7, Phaeobacter aquamixtae SSK6-1 T (Park et al, 2014); 8, Phaeobacter caeruleus 13 T (Vandecandelaere et al, 2009; Beyersmann et al, 2013; Park et al, 2014); 9, Phaeobacter daeponensis TF-218 T (Yoon et al, 2007; Vandecandelaere et al, 2008, 2009; Lucena et al, 2012; Dogs et al, 2013a; Park et al, 2014); 10, Leisingera nanhaiensis NH52F T (Sun et al, 2010; Jin et al, 2011; Breider et al, 2014); 11, Litorimicrobium taeanense G4 T (Jin et al, 2011); 12, Seohaeicola saemankumensis SD-15 T (Yoon et al, 2009); 13, Pelagicola litoralis CL-ES2 T (Kim et al, 2008); 14, Puniceibacterium antarcticum SM1211 T (Liu et al, 2014). Abbreviations used: DPG, Diphosphatidylglycerol; PC, Phosphatidylcholine; PE, Phosphatidylethanolamine; N.D., Not Determined.…”

Section: Resultsmentioning

confidence: 99%

“…The genus Leisingera was proposed by Schaefer et al (2002) and currently consists of three species, Leisingera methylohalidivorans (Schaefer et al, 2002), Leisingera aquimarina (Vandecandelaere et al, 2008), and Leisingera nanhaiensis (Sun et al, 2010). The strains belonging to these species were isolated from sea water, a marine electro-active biofilm and marine sandy sediment, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Genome-scale data suggest reclassifications in the Leisingera-Phaeobacter cluster including proposals for Sedimentitalea gen. nov. and Pseudophaeobacter gen. nov.

et al. 2014

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Earlier phylogenetic analyses of the marine Rhodobacteraceae (class Alphaproteobacteria) genera Leisingera and Phaeobacter indicated that neither genus might be monophyletic. We here used phylogenetic reconstruction from genome-scale data, MALDI-TOF mass-spectrometry analysis and a re-assessment of the phenotypic data from the literature to settle this matter, aiming at a reclassification of the two genera. Neither Phaeobacter nor Leisingera formed a clade in any of the phylogenetic analyses conducted. Rather, smaller monophyletic assemblages emerged, which were phenotypically more homogeneous, too. We thus propose the reclassification of Leisingera nanhaiensis as the type species of a new genus as Sedimentitalea nanhaiensis gen. nov., comb. nov., the reclassification of Phaeobacter arcticus and Phaeobacter leonis as Pseudophaeobacter arcticus gen. nov., comb. nov. and Pseudophaeobacter leonis comb. nov., and the reclassification of Phaeobacter aquaemixtae, Phaeobacter caeruleus, and Phaeobacter daeponensis as Leisingera aquaemixtae comb. nov., Leisingera caerulea comb. nov., and Leisingera daeponensis comb. nov. The genera Phaeobacter and Leisingera are accordingly emended.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-scale data suggest reclassifications in the Leisingera-Phaeobacter cluster including proposals for Sedimentitalea gen. nov. and Pseudophaeobacter gen. nov.

et al. 2014

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“…The highly virulent V. anguillarum strain 90‐11‐286 (Rønneseth et al ., 2017) was used in the co‐culture experiments and was isolated from diseased rainbow trout ( Oncorhynchus mykiss ) from a Danish aquaculture (Pedersen and Larsen, 1993; Skov et al ., 1995). P. inhibens DSM 17395 was used for the co‐culture experiments (Martens et al ., 2006; Vandecandelaere et al ., 2008; Buddruhs et al ., 2013). NB10 and 90‐11‐286 were grown and counted on TSA (Tryptone Soy Agar, Difco 212185) supplemented with 2.5 mg l −1 chloramphenicol for NB10.…”

Section: Methodsmentioning

confidence: 99%

Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems

Rasmussen

Erner

Bentzon-Tilia

et al. 2018

Microbial Biotechnology

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SummaryThe expanding aquaculture industry plays an important role in feeding the growing human population and with the expansion, sustainable bacterial disease control, such as probiotics, becomes increasingly important. Tropodithietic acid (TDA)‐producing Phaeobacter spp. can protect live feed, for example rotifers and Artemia as well as larvae of turbot and cod against pathogenic vibrios. Here, we show that the emerging live feed, copepods, is unaffected by colonization of the fish pathogen Vibrio anguillarum, making them potential infection vectors. However, TDA‐producing Phaeobacter inhibens was able to significantly inhibit V. anguillarum in non‐axenic cultures of copepod Acartia tonsa and the copepod feed Rhodomonas salina. Vibrio grew to 106 CFU ml−1 and 107 CFU ml−1 in copepod and R. salina cultures, respectively. However, vibrio counts remained at the inoculum level (104 CFU ml−1) when P. inhibens was also added. We further developed a semi‐strain‐specific qPCR for V. anguillarum to detect and quantify the pathogen in non‐axenic systems. In conclusion, P. inhibens efficiently inhibits the fish larval pathogen V. anguillarum in the emerging live feed, copepods, supporting its use as a probiotic in aquaculture. Furthermore, qPCR provides an effective method for detecting vibrio pathogens in complex non‐axenic live feed systems.

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“…nanhaiensis relative to the type strains of the other species within the genus Leisingera and the neighboring genera Phaeobacter , Oceanicola , and Seohaeicola [1-3,6-16]. The tree was inferred from 1,385 aligned characters of the 16S rRNA gene sequence under the maximum likelihood (ML) criterion as previously described [5].…”

Section: Classification and Featuresmentioning

confidence: 99%

“…The species name is referring to Nanhai, the Chinese name for the South China Sea. The other two Leisingera species were isolated from seawater and a marine electroactive biofilm, respectively [1,3]. All three Leisingera species are able to grow on methylated amines as the sole N source [4] and, at least for L.…”

Section: Introductionmentioning

confidence: 99%

Genome sequence and emended description of Leisingera nanhaiensis strain DSM 24252T isolated from marine sediment

Breider¹,

Teshima²,

Petersen³

et al. 2014

Stand. Genomic Sci.

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Leisingera nanhaiensis DSM 24252T is a Gram-negative, motile, rod-shaped marine Alphaproteobacterium, isolated from sandy marine sediments. Here we present the non-contiguous genome sequence and annotation together with a summary of the organism's phenotypic features. The 4,948,550 bp long genome with its 4,832 protein-coding and 64 RNA genes consists of one chromosome and six extrachromosomal elements with lengths of 236 kb, 92 kb, 61 kb, 58 kb, 56 kb, and 35 kb, respectively. The analysis of the genome showed that DSM 24252T possesses all genes necessary for dissimilatory nitrite reduction, and the strain was shown to be facultatively anaerobic, a deviation from the original description that calls for an emendation of the species. Also present in the genome are genes coding for a putative prophage, for gene-transfer agents and for the utilization of methylated amines. Phylogenetic analysis and intergenomic distances indicate that L. nanhaiensis might not belong to the genus Leisingera.

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Leisingera aquimarina sp. nov., isolated from a marine electroactive biofilm, and emended descriptions of Leisingera methylohalidivorans Schaefer et al. 2002, Phaeobacter daeponensis Yoon et al. 2007 and Phaeobacter inhibens Martens et al. 2006

Cited by 44 publications

References 22 publications

Genome-scale data suggest reclassifications in the Leisingera-Phaeobacter cluster including proposals for Sedimentitalea gen. nov. and Pseudophaeobacter gen. nov.

Genome-scale data suggest reclassifications in the Leisingera-Phaeobacter cluster including proposals for Sedimentitalea gen. nov. and Pseudophaeobacter gen. nov.

Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems

Genome sequence and emended description of Leisingera nanhaiensis strain DSM 24252T isolated from marine sediment

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