pH responsive cylindrical MSN for oral delivery of insulin-design, fabrication and evaluation

Abstract

Objective: The objective of the present study was to develop novel PMV [poly (methacrylic acidco-vinyl triethoxylsilane)]-coated mesoporous silica nanoparticles (MSN) with improved hypoglycemic effect for oral insulin (INS) delivery. Methods: MSN was synthesized under acidic condition using Pluronic Õ P 123 and Tetra ethoxy orthosilane. Surfactant was removed by calcination. Calcined MSN was coated with pH sensitive polymer PMV. Cytotoxicity of this coated MSN was evaluated by MTT assay using CHO-K1 cell line. Different MSN samples were characterized with BET surface area analyzer, FESEM, TEM, FT-IR, XRD, TG-DTA. In vivo study was performed using male rats. Pharmacokinetic study was conducted using HPLC. Results and discussion: Highest surface area (304.3921 m 2 /g) was observed in case of calcined sample. Adsorption pore width of final coated sample was highest (64.7844 nm) compared with others. No noticeable cytotoxicity was observed for this coated support. The entrapment efficiency of insulin was found to be 39.39%. In vitro studies were done at different pH using Franz-diffusion cell. Results showed significant release at pH 7.4. Cumulative drug release over a period of 6 h was more than 48% at this systemic pH. Effect of this MSN-PMV-INS on blood glucose level was retained for 16 h. This novel formulation has shown 73.10% relative bioavailability of insulin. Conclusion: A novel-coated mesoporous silica support was successfully developed for delivery of insulin through oral route.

Hybrid polymeric systems of mesoporous silica/hydroxyapatite nanoparticles applied as antitumor drug delivery platform

“…The inhibition of the reduction of dihydrofolate to tetrahydrofolate causes blocking of the DNA synthesis. 29,30 However, studies exploring the multifunctional nanosystems composed of mesoporous silica, hydroxyapatite, TEGDMA, MAA, and copper ions with methotrexate loading that was proposed in this study, were not found in the literature. 27 Although high-dose MTX is frequently used in the treatment, conventional dose therapy is ineffective.…”

“…The MAA polymer has been the object of several studies of pH-sensitive functionalized nanoparticles and has also been found in applications coupled with mesoporous silica. 29,30 However, studies exploring the multifunctional nanosystems composed of mesoporous silica, hydroxyapatite, TEGDMA, MAA, and copper ions with methotrexate loading that was proposed in this study, were not found in the literature. Our results indicate that functionalized Cu-MSN/ HA nanocomposite are promising for biomedical applications especially as pH-sensitive nanocarriers for tumor-targeted drug delivery.…”

Anti-GPC3 antibody-modified sorafenib-loaded nanoparticles significantly inhibited HepG2 hepatocellular carcinoma

“…To solve these problems, we added Pluronic ® P123 to PCL. Pluronic compounds are widely used in the biomedical field for purposes ranging from drug delivery to medical imaging (Guha et al., 2016 ; Forget et al., 2017 ; Liu et al., 2017 ). Pluronic P123 can be added to PCL as a nucleating agent to enhance drug release.…”

Inkjet Printing of Drug-Loaded Mesoporous Silica Nanoparticles—A Platform for Drug Development

“…These materials have shown to enable improvement or added functionality of previous compounds and could also have potential in the development of super generics [ 32 ]. Such studies showing improved delivery and usability of poorly soluble, poorly permeable, very potent APIs [ 33 ], biomolecules [ 34 ], proteins [ 35 ], peptides [ 36 ], antibodies [ 37 ], and genes [ 38 ] have been published to date.…”