Perubahan morfologi sel HeLa setelah paparan ekstrak etanolik Curcuma longa

Hutomo, Suryani; Susilowati, Heni; Suryanto, Yanti Ivana; Kurniawan, Chandra

doi:10.22146/majkedgiind.11248

Cited by 5 publications

(3 citation statements)

References 4 publications

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“…On the other side, the morphology of living cells can be seen in that the cell nucleus shines brilliantly and the membrane boundaries with the media are visible. Thus extract C.sappan L can be used as an alternative ingredient for the prevention or reduction of risk affected by cancer [20,21,22]. Secondary metabolites are compounds that are not directly involved in organisms' growth, development, or reproduction.…”

Section: Activity Test Results Against Lung Cancer Cells (Cell A549)mentioning

confidence: 99%

Antibacterial and Anticancer Activities of Acetone Extract Caesalpinia sappan L.

Juwitaningsih

Syahputra²,

Eddiyanto

et al. 2023

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Sappan wood (Caesalpinia sappan L.) from the Caesalpiniaceae family is a plant that has been widely used as a health drink such as herbal medicine, in Sundanese called jamu or wedang secang. The drink containing C.sappan extract is traditionally believed to have the ability to reduce symptoms of colds, coughs, canker sores, and rheumatism, overcome fatigue and improve blood circulation. In this research, wood extract of C. sappan L in acetone solvent tested as antibacterial and anticancer. The antibacterial test was performed on the bacteria Streptococcus mutans ATCC 35668 and Enterococcus. faecalis ATCC 49619 with paper disc diffusion method. The microdilution method was used to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The anticancer test is carried out in vitro on A549 lung cancer cells using the MTT method assay. The results showed that the wood extract of C. sappan L in acetone has weak antibacterial activity against S. mutans ATCC 35668 and E. faecalis ATCC 49619. The MIC is 1250 ug/mL, respectively. Aceton wood extract of C. sappan L. showed moderate anticancer activity with IC50 value 90,01µg/mL. Thus, the extract of C. sappan L. in acetone has the potential as a source of anticancer compounds.

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Section: Activity Test Results Against Lung Cancer Cells (Cell A549)mentioning

confidence: 99%

Antibacterial and Anticancer Activities of Acetone Extract Caesalpinia sappan L.

Juwitaningsih

Syahputra²,

Eddiyanto

et al. 2023

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“…Normal HeLa cells are polygonal or round with extensive cytoplasm. 31 After being given the treatment, the cells became spherical and disconnected from each other and showed cellular shrinkage and nuclear condensation. 32 Cell shrinkage as a result of cell dehydration.…”

Section: Effect Of Gnetum Gnemon L Seed Fractions On Viability Hela C...mentioning

confidence: 99%

Antioxidant and Cytotoxic Activities of Melinjo (Gnetum gnemon L.) Seed Fractions on HeLa Cell Line an In Vitro

Sukohar¹,

Suharyani²,

Sutyarso³

et al. 2022

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Introduction: Medicinal plants have been investigated for possible anti-cancer effects. One of them is Gnetum gnemon L (melinjo). This study aims determined in vitro antioxidant activity and the cytotoxic effects of polar, semipolar and non polar melinjo seed fractions againts HeLa cell line. Methods: The melinjo seed were extracted with ethanol as a solvent. Then, the fractionation was done using liquidliquid extraction method with three different polarity solvent. Cytotoxic activity was carried out using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay in HeLa cell lines at concentrations ranging from 25 to 400 μg/mL. Antioxidant activity was determined by the diphenyl picryl hidrazil (DPPH) radical scavenging method. Results: Phytochemical screening indicated the presence of phyto-constituents like flavonoids, terpenoid and tanin. The DPPH scavenging activity by the melinjo seed aqueous, ethyl acetate and N-hexane fraction was 733,12 ± 18,95 μg/mL; 68,40 ± 1,9 μg/mL and 2035,70 ± 65,59 μg/mL, respectively. The cytotoxic activity of the melinjo seed fractions showed that the ethyl acetate was the most active fraction against HeLa cell line with IC 50 value 45,27 μg/mL. Conclusion: In this study, we have observed that the melinjo seed fractions exhibited antioxidant and cytotoxic activity against HeLa cell lines. This is presumably due to the content of phytochemicals and stilbenoids such as resveratrol and gnetin C. Melinjo seeds are more potent as anticancer Compared with other plants that also contain RSV such as grape extract (Vitis vinifera L.) against lung cancer cells (A549). From the three fractions, the ethyl acetate fraction had the highest antioxidant and cytotoxic effect compared to the water and n-hexane fractions. Gnetum gnemon L. can be considered as a potential source of anticancer agents. However, more research is needed to determine the mechanism of action.

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“…Penelitian terdahulu juga melaporkan bahwa kurkumin menyebabkan kematian sel sebanyak separuh dari jumlah sel yang diinduksinya, meskipun mekanismenya belum jelas. 16 Kematian sel dapat dipengaruhi oleh sifat dari suatu bahan kemurnian, struktur kristal, komposisi kimia, media yang digunakan dan kelarutan atau kemampuan bahan melepaskan suatu ion. 17…”

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Sitotoksisitas Kombinasi Seng-oksida dan Ekstrak Curcuma longa Terhadap Fibroblas Gingiva Manusia

Aprilia¹,

Meizarini²,

Agustantina³

2019

JMKG

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The use of zinc oxide - eugenol as periodontal dressing is controversial due to the toxic nature of eugenol and Curcuma longa Linn as a safe natural herbal, is expected to replace eugenol function. Previous research has proven that the combination of zinc oxide with Curcuma liquid extract has an antibacterial properties and the use of those as wound dressing through in vivo studies proved to accelerate wound healing. The ingredients applied to the oral cavity must benon-toxic and biocompatible, therefore cytotoxicity testing 1 is required. To determine the cytotoxicity of zinc oxide and Curcuma longa extract combination on human gingival fibroblast cells. Cytotoxicity test of combination of zinc oxide powder with Curcuma liquid extract was done at ratio of 1: 1 (group A) and 2: 1 (group B), Curcuma liquid extract (group C) and zinc oxide powder (group D), which performed on human gingival fibroblast culture-cells (3-5x103 densities) in 96 wells microplate. MTT essay was used to analyzed with IC50 parameters. Statistical analysis using oneway Anova and LSD. The number of living cells in group A = 64.819%, group B = 59.452%, group C = 52.859% and group D = 60.502%. There was no significant difference between the groups of A, B, D but those groups were significantly different to group C. Combination of zinc oxide with Curcuma extract ratio of 1: 1 and 2: 1, zinc oxide powder and Curcuma liquid extract are not toxic to human gingival fibroblast at IC50.

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Perubahan morfologi sel HeLa setelah paparan ekstrak etanolik Curcuma longa

Cited by 5 publications

References 4 publications

Antibacterial and Anticancer Activities of Acetone Extract Caesalpinia sappan L.

Antibacterial and Anticancer Activities of Acetone Extract Caesalpinia sappan L.

Antioxidant and Cytotoxic Activities of Melinjo (Gnetum gnemon L.) Seed Fractions on HeLa Cell Line an In Vitro

Sitotoksisitas Kombinasi Seng-oksida dan Ekstrak Curcuma longa Terhadap Fibroblas Gingiva Manusia

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