Persistence of Human Papillomavirus DNA inCervical Lesions After Treatment With DiathermicLarge Loop Excision

Distéfano, A. L.; Picconi, Marı́a Alejandra; Alonio, Lidia Virginia; Dalbert, D.; Mural, Juan; Bartt, O.; Bazán, Graciela; Cervantes, Gloria; Lizano, Marcela; Carrancá, Alejandro García; Teyssié, Angélica R.

doi:10.1155/s106474499800043x

Cited by 13 publications

(16 citation statements)

References 14 publications

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“…PCR with generic primers, combined with single-stranded conformational polymorphism (SSCP) analysis, has been applied to HPV detection and typing in cervical samples [Lizano et al, 1997;Distéfano et al, 1998]. …”

Section: Introductionmentioning

confidence: 99%

Human papillomavirus (HPV) DNA in penile carcinomas in Argentina: Analysis of primary tumors and lymph nodes

Picconi

Eiján

Distéfano³

et al. 2000

J. Med. Virol.

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Among sexually transmitted diseases, infection by human papillomavirus (HPV) has become one of the most important. On the other hand, though epidemiological data show that some HPV types are closely associated with cervical cancer, few reports have been found with reference to penile carcinoma because of its rare occurrence. The aim of this study was to investigate the relationship between HPV infection and penile cancer in Argentina. A retrospective study was carried out on 38 white men with penile squamous-cell carcinoma. Sixty-five archival fixed biopsies taken from 34 primary penile tumors, 25 nodal metastases, 1 skin "satellite" metastasis and 5 histologically normal lymph nodes were used as specimens. HPV detection and typing were carried out by the polymerase chain reaction (PCR) using generic primers, combined with single-stranded conformational polymorphism (SSCP) analysis. HPV DNA was found in 71% patients, corresponding 81% of them to "high risk" types, with predominance of HPV 18. Both primary tumors and metastases showed concordance of HPV occurrence and type in both lesions. In 3 patients, HPV 16 was detected not only in primary tumors and metastases, but also in histologically normal lymph nodes. Our data indicate that most penile carcinomas in Argentine patients are etiologically related to HPV, especially to "high risk" genital types. The agreement in HPV detection between primary tumors and metastases suggests a potential viral role in tumor progression. HPV detection in otherwise histologically normal lymph nodes might be useful as early marker of a metastatic process.

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Section: Introductionmentioning

confidence: 99%

Human papillomavirus (HPV) DNA in penile carcinomas in Argentina: Analysis of primary tumors and lymph nodes

Picconi

Eiján

Distéfano³

et al. 2000

J. Med. Virol.

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“…Successful treatment for CIN is associated with HPV clearance (11). HPV DNA persistence is known to be a near-necessary risk factor for CIN (3,4,15,21,24,34,40), and as expected, posttreatment presence of HR-HPV is a good marker for recurrence (10,17), better than abnormal cytology (26).…”

Section: Discussionmentioning

confidence: 92%

Comparison between the Hybrid Capture II Test and a PCR-Based Human Papillomavirus Detection Method for Diagnosis and Posttreatment Follow-Up of Cervical Intraepithelial Neoplasia

et al. 2005

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Human papillomavirus (HPV) infection is the major cause of cervical cancer and its precursor, cervical intraepithelial neoplasia (CIN), and HPV testing has therefore been proposed for improved triaging and follow-up of women treated for CIN. We compared two common HPV DNA detection tests (Hybrid Capture II [HCII] and PCR-enzyme immunosorbent assay (EIA) using the primers GP5؉/GP6؉ followed by HPV typing with reverse dot blot hybridization) for sensitivity and specificity for detection of CIN and of CIN recurrence after treatment. Two hundred and thirty-nine women referred to the Department of Obstetrics and Gynaecology in Västerås, Sweden, were enrolled because of atypical Pap smears; 177 of these were later treated for dysplasia by conization or loop diathermy. Samples for HPV DNA testing were taken before and 4 to 6 months after treatment. There was substantial agreement between the HCII and PCR-EIA (kappa, 0.70 before treatment and 0.72 after treatment). The sensitivity for histopathologically confirmed CIN III was 100.0% for PCR-EIA and 95.6% for HCII. For patients with CIN II or worse (CIN II؉), the sensitivities were 92.9% (PCR-EIA) and 91.8% (HCII). The specificities for CIN II؉ in the pretreatment setting were 30.4% for PCR-EIA and 24.1% for HCII. After treatment, the sensitivities for CIN III in cytology were 100.0% by both methods, and for CIN II؉, sensitivities were 80.0% by both methods. The specificities for CIN II؉ in the posttreatment setting were 83.5% for PCR and 85.4% for HCII. In conclusion, the sensitivities of both PCR-EIA and HCII are high and almost equal, suggesting that both methods are suitable as tools for detection and posttreatment follow-up of CIN II-III.Cancer of the cervix is the third most common form of cancer among women worldwide, with almost 400,000 new cases each year (28). The principal cause of invasive cervical cancer and its precursor lesions is infection with oncogenic types of human papillomavirus (HPV) (3,4,15,21,24,34,40), which is found in close to 100% of cancers (39). A recent case control study reported that HPV types 16,18,31,33,35,39, 45, 51, 52, 56, 58, 59, 68, 73, and 82 conferred increased risk for cervical cancer and that HPV types 6,11,40, 42, 43, 44, 54, 61, 70, 72, and 81 did not increase the cervical cancer risk (23). A meta analysis of all studies of cervical cancer found that the most important HPV type in squamous cell carcinomas is HPV 16, followed by HPV 18, 45, 31, and 33 (7). In adenocarcinomas, the most common type is HPV 18 followed by HPV 16 and 45 (7).In young women, the incidence of HPV infection is high, but the infection is often of short duration (16). The prevalence of high-risk (HR) HPV DNA is highest among sexually active teenagers, declining in women 20 to 30 years of age, and is still lower in women over the age of 30 (4, 19). Persistent HR HPV infections, which are more common in older women, greatly increase the risk of cervical intraepithelial neoplasia (CIN) (33) or cervical cancer, especially if the viral load is high (15,16,3...

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“…Os autores não detectaram o DNA-HPV nas 22 pacientes sem recidiva após a conização pertencentes ao grupo controle. Da mesma forma, Distefano et al 11 …”

Section: Discussionunclassified

“…Não se sabe entretanto, se a detecção do DNA-HPV após o tratamento da NIC, pode ser utilizada como fator clí-nico preditivo de persistência ou recidiva da doença. Muitos autores acreditam que a detecção do DNA-HPV ajuda a selecionar as mulheres que apresentarão recidiva, porém outros não verificaram associação entre a detecção viral e a presença de doença residual ou recorrente [9][10][11][12][13][14][15] .…”

Section: Introductionunclassified

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IntroduçãoA excisão da zona de transformação com alça diatérmica é hoje uma das técnicas cirúrgicas mais utilizadas para o diagnóstico e tratamento da neoplasia intra-epitelial cervical (NIC). Existe correlação entre o comprometimento das margens da peça cirúrgica e a freqüência de doença residual e de recidiva.

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Detecção do DNA do papilomavírus humano após excisão da zona de transformação com alça diatérmica para tratamento de neoplasia intra-epitelial cervical

Figueirêdo¹,

Derchain²,

Sarian³

et al. 2003

Rev. Bras. Ginecol. Obstet.

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RESUMO Objetivo: avaliar a presença do DNA do papilomavírus humano (HPV) de alto risco oncológico antes e quatro meses após excisão da zona de transformação com alça diatérmica em mulheres com neoplasia intra-epitelial cervical (NIC (OR = 4,8; IC 95% = 1,(7)(8)(9)(10)(11)(12)(13)7), porém não se relacionou com doença residual ou recidiva histológica (OR = 6,0; IC 95% = 0,3 IntroduçãoA excisão da zona de transformação com alça diatérmica é hoje uma das técnicas cirúrgicas mais utilizadas para o diagnóstico e tratamento da neoplasia intra-epitelial cervical (NIC). Existe correlação entre o comprometimento das margens da peça cirúrgica e a freqüência de doença residual e de recidiva. Porém, se 30% das mulheres com margens comprometidas apresentam doença residual no colo restante, 8% daquelas com margens livres, também apresentem doença residual ou recidiva 1-3 . Assim, atualmente, se tem optado pelo seguimento das mulheres submetidas à conização por NIC independentemente do comprometimento das margens do cone, com a coleta de colpocitologia oncológica (CO) e realização de colposcopia. Porém, um grande impulso vem sendo dado às pesquisas que possam identificar um novo marcador de recidiva após a conização [4][5][6] .Está bem estabelecido o papel do papiloma-

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Persistence of Human Papillomavirus DNA inCervical Lesions After Treatment With DiathermicLarge Loop Excision

Abstract: Objective: The aim of this study was to identify human papillomavirus (HPV)

Cited by 13 publications

References 14 publications

Human papillomavirus (HPV) DNA in penile carcinomas in Argentina: Analysis of primary tumors and lymph nodes

Human papillomavirus (HPV) DNA in penile carcinomas in Argentina: Analysis of primary tumors and lymph nodes

Comparison between the Hybrid Capture II Test and a PCR-Based Human Papillomavirus Detection Method for Diagnosis and Posttreatment Follow-Up of Cervical Intraepithelial Neoplasia

Detecção do DNA do papilomavírus humano após excisão da zona de transformação com alça diatérmica para tratamento de neoplasia intra-epitelial cervical

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