Persian Gulf Snail Crude Venom (Conus textile): A Potential Source of Anti-Cancer Therapeutic Agents for Glioblastoma through Mitochondrial-Mediated Apoptosis

Salimi, Ahmad; Rahimitabar, Niloofar; Vazirizadeh, Amir; Adhami, Vahed; Pourahmad, Jalal

doi:10.31557/apjcp.2021.22.s1.49

Cited by 8 publications

(11 citation statements)

References 27 publications

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“…The results of our study showed that fraction B obtained from Conus textile was able to increase the level of ROS in CLL. These results are in agreement with the results of previous studies that have shown that crude venom of Conus textile and other animal venoms have the ability to increase the level of ROS (Al-Asmari et al, 2018;Salimi et al, 2020).…”

Section: Fraction B Increased Ros Generation In Cll Blymphocytessupporting

confidence: 93%

“…Therefore, an increase in the level of ROS can kill cancer cells (Seydi et al, 2016;Seydi et al, 2018). Our previous study showed that crude venom of Conus textile has the ability to increase ROS and induction of apoptosis signaling in U87MG human glioma cells through the mitochondrial pathway (Salimi et al, 2020). This research was aimed to investigate the apoptotic effects of crude venom of Conus textile and its fractions (A and B) on CLL.…”

Section: Cytotoxicity Studies Of the Crude Venom And Fractions Of Persian Gulf Snail (Conus Textile) On Chronic Lymphocytic Leukemia And mentioning

confidence: 99%

“…The Conus textile samples were collected from Larak Island in the Persian Gulf, in the south of Iran. The stages of toxin extraction are described in our previous study (Salimi et al, 2020).…”

Section: Collection and Extraction Of Venommentioning

confidence: 99%

“…In the following, we removed the DCFH-DA reagent and re-washed the cells with PBS. Finally, fluorescence intensity (DCF) was evaluated using flow cytometry (Cyflow Space-Partec, Germany) and the mean of fluorescence intensity was analyzed by software (FlowJo) (Salimi et al, 2020).…”

Section: Ros Assaymentioning

confidence: 99%

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Cytotoxicity Studies of the Crude venom and Fractions of Persian Gulf Snail (Conus Textile) on Chronic Lymphocytic Leukemia and Normal Lymphocytes

Salimi

Salehian

Aboutorabi³

et al. 2021

Asian Pac J Cancer Prev

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Background: Marine animals have been considered by many researchers due to their various pharmacological effects. One group of marine animals that have been studied is cone snails. The conotoxin obtained from these marine animals has various therapeutic effects. Methods: This study was designed to investigate the apoptotic effects of crude venom of Conus textile and its fractions (A and B) on chronic lymphocytic leukemia (CLL) cells. Accordingly, parameters such as cell viability, reactive oxygen species (ROS) level, collapse in mitochondrial membrane potential (MMP), lysosomal membrane damage and caspase-3 activation were evaluated. Results: The results showed that the crude venom (50, 100 and 200 µg/ml) from Conus textile and its fraction B (50, 100 and 200 µg/ml) significantly reduced viability in CLL B-lymphocyte. In addition, exposure of CLL B-lymphocyte to fraction B (50, 100 and 200 µg/ml) was associated with an increase in the level of ROS, the collapse of the MMP, damage to the lysosomal membrane, and activation of caspase-3. Conclusion: According to results, it was concluded that fraction B from crude venom of Conus textile causes selective toxicity on CLL B-lymphocyte with almost no effect on a normal lymphocyte. Furthermore, this venom fraction could be a promising candidate for induction of apoptosis in patients with CLL through the mitochondrial pathway.

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Section: Fraction B Increased Ros Generation In Cll Blymphocytessupporting

confidence: 93%

Section: Cytotoxicity Studies Of the Crude Venom And Fractions Of Persian Gulf Snail (Conus Textile) On Chronic Lymphocytic Leukemia And mentioning

confidence: 99%

Section: Collection and Extraction Of Venommentioning

confidence: 99%

Section: Ros Assaymentioning

confidence: 99%

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Cytotoxicity Studies of the Crude venom and Fractions of Persian Gulf Snail (Conus Textile) on Chronic Lymphocytic Leukemia and Normal Lymphocytes

Salimi

Salehian

Aboutorabi³

et al. 2021

Asian Pac J Cancer Prev

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“…Cassiopea Andromeda venom fractions induce injury in mitochondria obtained from breast adenocarcinoma patients (Mirshamsi et al, 2017b). Conus Textile venom affects glioblastoma through mitochondrial-mediated apoptosis pathway (Salimi et al, 2020). Marine Mollusc (Turbo Coronatus) induces apoptosis in mouse melanoma cells (Zangeneh et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Anti-Glioma Effect of Pseudosynanceia Melanostigma Venom on Isolated Mitochondria from Glioblastoma Cells

Ramezani

Samiei

Pourahmad

2021

Asian Pac J Cancer Prev

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Background: Glioblastoma is the most common primary malignant tumor of the central nervous system that occurs in the spinal cord or brain. Pseudosynanceia Melanostigma is a venomous stonefish in the Persian Gulf, which our knowledge about is little. This study's goal is to investigate the toxicity of stonefish crude venom on mitochondria isolated from U87 cells. Methods: In the first stage, we extracted venom stonefish and then isolated mitochondria have exposed to different concentrations of venom. Finally, mitochondrial toxicity parameters (Succinate dehydrogenase (SDH) activity, Reactive oxygen species (ROS), cytochrome c release, Mitochondrial Membrane Potential (MMP), and mitochondrial swelling) have evaluated. Results: To determine mitochondrial parameters, we used 115, 230, and 460 µg/ml concentrations. The results of our study show that the venom of stonefish selectively increases upstream parameters of apoptosis such as mitochondrial swelling, cytochrome c release, MMP collapse and ROS. Conclusion: This study suggests that Pseudosynanceia Melanostigma crude venom has selectively caused toxicity by increasing active mitochondrial oxygen radicals. This venom could potentially be a candidate for the treatment of glioblastoma.

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Why to Study Peptides from Venomous and Poisonous Animals?

Oliveira

Soares

Silva

2023

Int J Pept Res Ther

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Venom and poison peptides are powerful biological weapons and have proven immense pharmacological potential because of their high binding affinity to a wide range of molecular targets. Nonetheless, many of these peptides cannot directly be used as medicines due to their toxicity but their derivatives are very valuable to explore and can be a great treasure trove for the development of novel drugs. This review presents a detailed overview of venom peptides present in reptiles, amphibians, arachnids, gastropods, clitellatas, fish, insects, and mammals. We address the most recent findings that underline their therapeutic potential against a wide variety of diseases from cancer to vascular, autoimmune, and inflammatory diseases. Graphical Abstract

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Persian Gulf Snail Crude Venom (Conus textile): A Potential Source of Anti-Cancer Therapeutic Agents for Glioblastoma through Mitochondrial-Mediated Apoptosis

Cited by 8 publications

References 27 publications

Cytotoxicity Studies of the Crude venom and Fractions of Persian Gulf Snail (Conus Textile) on Chronic Lymphocytic Leukemia and Normal Lymphocytes

Cytotoxicity Studies of the Crude venom and Fractions of Persian Gulf Snail (Conus Textile) on Chronic Lymphocytic Leukemia and Normal Lymphocytes

Anti-Glioma Effect of Pseudosynanceia Melanostigma Venom on Isolated Mitochondria from Glioblastoma Cells

Why to Study Peptides from Venomous and Poisonous Animals?

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