Peroxidation due to cryoprotectant treatment is a vital factor for cell survival in Arabidopsis cryopreservation

Effect of antioxidants on the genetic stability of cryopreserved mint shoot tips by encapsulation–dehydration

“…When the response after cryopreservation by encapsulation-dehydration of two Ribes species was compared, the species that showed higher recovery after cryopreservation displayed higher antioxidant activity (Johnston et al 2007). Furthermore, differential response to cryopreservation (by vitrification with PVS2) of Arabidopsis thaliana 48-and 72 h-seedlings (the latter being more sensitive) was related to the expression of oxidative stress response genes (Ren et al 2013). However, ROS, at low concentrations, regulate cellular functions and act as signalling molecules and, in this line, an increased ROS generation was found to be related to improved viability after cryopreservation of Lilium × siberia pollen (Xu et al 2014).…”

Transcriptomic profiling revealed the regulatory mechanism of Arabidopsis seedlings response to oxidative stress from cryopreservation

“…The accumulation of ROS was observed in cryopreserved Brassica napus (Benson and Noronha-Dutra 1988), Ribes (Johnston et al 2007), Theobroma cacao (Fang et al 2008), Trichilia dregeana (Whitaker et al 2010) and Hypericum perforatum (Skyba et al 2012), and ROS-induced oxidative stress can make a significant impact and reduce post-cryopreservation regrowth even when an optimized protocol is applied. Membrane lipids are primary targets for oxidative stress (Benson and Bremner 2004), and malondialdehyde (MDA) as a breakdown product of lipid peroxidation increased in cryopreserved Oryza sativa (Benson et al 1992), Azadirachta indica (Varghese and Naithani 2008), Zea mays (Wen et al 2010), Dendrobium wardianum (Wu 2011), and Arabidopsis thaliana (Ren et al 2013). Ren et al (2013) also found that the regrowth ability of cryostored Arabidopsis seedlings was negatively correlated with the accumulation of MDA.…”

“…Membrane lipids are primary targets for oxidative stress (Benson and Bremner 2004), and malondialdehyde (MDA) as a breakdown product of lipid peroxidation increased in cryopreserved Oryza sativa (Benson et al 1992), Azadirachta indica (Varghese and Naithani 2008), Zea mays (Wen et al 2010), Dendrobium wardianum (Wu 2011), and Arabidopsis thaliana (Ren et al 2013). Ren et al (2013) also found that the regrowth ability of cryostored Arabidopsis seedlings was negatively correlated with the accumulation of MDA. However, plants possess complex antioxidant systems including many enzymatic and non-enzymatic antioxidants to resist oxidative stress (Halliwell 2006), and high cellular antioxidant status is associated with tolerance to cryopreservation (Volk 2010).…”

“…The steps in vitrification can impose a series of stresses on plants arising from excision, osmotic injury, dehydration and changes in temperature (Uchendu et al 2010a;Ren et al 2013). These factors lead to the accumulation of reactive oxygen species (ROS) (Uchendu et al 2010a, b).…”

“…Some studies utilized exogenous antioxidants to elevate antioxidant status, and found that the addition of Vitamin C (VC), tocopherol, lipoic acid or glutathione (GSH) significantly increased regrowth of Citrus shoot tips (Wang and Deng 2004), blackberry shoot tips (Uchendu et al 2010a, b) and Arabidopsis seedlings (Ren et al 2014) after cryopreservation. Thus, oxidative stress plays a pivotal role in cryopreservation; reducing oxidative damages is an effective way to improve recovery after cryopreservation (Ren et al 2013).…”