Plant Cryopreservation for Biotechnology and Breeding

Попова, Елена; Shukla, Mukund R.; Kim, Haeng Hoon; Saxena, Praveen K.

doi:10.1007/978-3-319-22521-0_3

Cited by 27 publications

(22 citation statements)

References 110 publications

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“…Worldwide there are over 700,000 cryopreserved accessions of crop species representing 13.12% of the total number preserved in germplasm banks [ 49 ]. Besides, cryopreservation is often considered as the only effective method to prevent cell ageing and reduce the risks of culture loss caused by contamination or technical errors when preserving in vitro cultures of undifferentiated somatic plant cells [ 93 ], used as a source of phytochemicals for food and pharmaceutical industries.…”

Section: Cryopreservationmentioning

confidence: 99%

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

González‐Benito

Ibáñez

Pirredda

et al. 2020

IJMS

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Epigenetic variation, and particularly DNA methylation, is involved in plasticity and responses to changes in the environment. Conservation biology studies have focused on the measurement of this variation to establish demographic parameters, diversity levels and population structure to design the appropriate conservation strategies. However, in ex situ conservation approaches, the main objective is to guarantee the characteristics of the conserved material (phenotype and epi-genetic). We review the use of the Methylation Sensitive Amplified Polymorphism (MSAP) technique to detect changes in the DNA methylation patterns of plant material conserved by the main ex situ plant conservation methods: seed banks, in vitro slow growth and cryopreservation. Comparison of DNA methylation patterns before and after conservation is a useful tool to check the fidelity of the regenerated plants, and, at the same time, may be related with other genetic variations that might appear during the conservation process (i.e., somaclonal variation). Analyses of MSAP profiles can be useful in the management of ex situ plant conservation but differs in the approach used in the in situ conservation. Likewise, an easy-to-use methodology is necessary for a rapid interpretation of data, in order to be readily implemented by conservation managers.

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Section: Cryopreservationmentioning

confidence: 99%

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

González‐Benito

Ibáñez

Pirredda

et al. 2020

IJMS

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“…Cryopreservation refers to the storage of diverse living materials at below –130°C ( Engelmann, 2004 ). It serves as an alternative conservation approach to the conventional field and in vitro (i.e., slow growth) germplasm conservation and is cost-effective over extended periods with minimal space and routine maintenance requirements ( Pence, 2011 ; Engelmann, 2014 ; Popova et al, 2015 ). It also assists current breeding programs by providing long-term storage and an easy long-distance exchange of genetic materials (e.g., pollen and meristematic apices and buds).…”

Section: Cryopreservationmentioning

confidence: 99%

“…It also assists current breeding programs by providing long-term storage and an easy long-distance exchange of genetic materials (e.g., pollen and meristematic apices and buds). Cryopreservation has been implemented for various plant species using different methods, the most popular and widely applicable, including controlled freezing, vitrification, encapsulation-dehydration, encapsulation-vitrification, and droplet-vitrification ( Sakai and Engelmann, 2007 ; Popova et al, 2015 ). These methods follow distinct approaches to dehydrate cryopreserving living materials by converting liquid water to a glassy state to avoid the lethal formation of intracellular ice.…”

Section: Cryopreservationmentioning

confidence: 99%

Medical Cannabis and Industrial Hemp Tissue Culture: Present Status and Future Potential

Adhikary

Kulkarni

El-Mezawy³

et al. 2021

Front. Plant Sci.

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In recent years high-THC (psychoactive) and low-THC (industrial hemp) type cannabis (Cannabis sativa L.) have gained immense attention in medical, food, and a plethora of other consumer product markets. Among the planting materials used for cultivation, tissue culture clones provide various advantages such as economies of scale, production of disease-free and true-to-type plants for reducing the risk of GMP-EuGMP level medical cannabis production, as well as the development and application of various technologies for genetic improvement. Various tissue culture methods have the potential application with cannabis for research, breeding, and novel trait development, as well as commercial mass propagation. Although tissue culture techniques for plant regeneration and micropropagation have been reported for different cannabis genotypes and explant sources, there are significant variations in the response of cultures and the morphogenic pathway. Methods for many high-yielding elite strains are still rudimentary, and protocols are not established. With a recent focus on sequencing and genomics in cannabis, genetic transformation systems are applied to medical cannabis and hemp for functional gene annotation via traditional and transient transformation methods to create novel phenotypes by gene expression modulation and to validate gene function. This review presents the current status of research focusing on different aspects of tissue culture, including micropropagation, transformation, and the regeneration of medicinal cannabis and industrial hemp transformants. Potential future tissue culture research strategies helping elite cannabis breeding and propagation are also presented.

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“…Cryopreservation, i.e., the preservation of biological material at ultralow temperatures (in liquid nitrogen and/or its vapor phase at temperatures ca. −196 °C to −140 °C [ 3 ]), is a useful and cost-effective option to secure the long-term preservation of plant germplasm [ 4 , 5 , 6 ]. For example, in the case of clonally propagated fruit trees that are traditionally maintained in clonal orchards, the utilization of cryopreservation can greatly improve the conservation of germplasm [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Successful Cryopreservation of Dormant Buds of Blackcurrant (Ribes nigrum L.) by Using Greenhouse-Grown Plants and In Vitro Recovery

Rantala

Kaseva

Nukari

et al. 2021

Plants

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The cryopreservation of dormant buds can be a feasible method for preserving germplasm of cold-tolerant woody plants. In the present study, we evaluated the effects of pre-desiccation, thawing method, and the rehydration of bud sections on the post-cryopreservation recovery of dormant blackcurrant buds in vitro. The estimated recovery of small- and medium-sized buds was 80.1 and 62.7% respectively for desiccated buds and 67.8 and 72.3% respectively for non-desiccated buds. The pre-desiccation of bud sections enhanced the number of the shoots regenerated from vegetative buds (2.3 vs. 4.7). The estimated recovery of fast-thawed buds was better after 14-day than after 7-day rehydration (85 vs. 59%). In slowly thawed buds the difference between 14-day and 7-day rehydration was not significant (73 vs. 62%). The estimated recovery of vegetative and flower buds was 77.7 and 41.1% respectively after 7-day rehydration, and 95.2 and 43.6% respectively after a 14-day rehydration period. The rehydration of bud sections was not necessary for the in vitro recovery of non-desiccated, fast-thawed buds. Of the 23 blackcurrant cultivars cryopreserved using non-desiccated dormant buds collected from a greenhouse, the estimated recovery of 22 cultivars ranged between 42 and 90%.

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Plant Cryopreservation for Biotechnology and Breeding

Cited by 27 publications

References 110 publications

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

Medical Cannabis and Industrial Hemp Tissue Culture: Present Status and Future Potential

Successful Cryopreservation of Dormant Buds of Blackcurrant (Ribes nigrum L.) by Using Greenhouse-Grown Plants and In Vitro Recovery

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