Transcriptomic profiling revealed the regulatory mechanism of Arabidopsis seedlings response to oxidative stress from cryopreservation

Abstract

Elevated antioxidant status and positive abiotic stress response in dehydration enhance cell resistance to cryoinjury, and controlling oxidative damage via reactive oxygen species homeostasis maintenance leads to high survival. Cryoprotectants are important for cell survival in cryopreservation, but high concentrations can also cause oxidative stress. Adding vitamin C to the cryoprotectant doubled the survival ratio in Arabidopsis thaliana 60-h seedlings (seedlings after 60-h germination) cryopreservation. In this study, the metabolites and transcriptional profiling of 60-h seedlings were analyzed in both the control cryopreservation procedure (CCP) and an improved cryopreservation procedure (ICP) to reveal the mechanism of plant cell response to oxidative stress from cryopreservation. Reactive oxygen species (ROS) and peroxidation levels reached a peak after rapid cooling-warming in CCP, which were higher than that in ICP. In addition, gene regulation was significantly increased in CCP and decreased in ICP during rapid cooling-warming. Before cryogenic treatment, the number of specifically regulated genes was nearly 10 times higher in ICP dehydration than CCP dehydration. Among these genes, DREBs/CBFs were beneficial to cope with cryoinjury, and calcium-binding protein, OXI1, WRKY and MYB family members as key factors in ROS signal transduction activated the ROS-producing and ROS-scavenging networks including AsA-GSH and GPX cycles involved in scavenging H2O2. Finally, elevated antioxidant status and oxidative stress response in the improved dehydration enhanced seedling resistance to cryogenic treatment, maintained ROS homeostasis and improved cell recovery after cryopreservation.

Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species

“…A more recent area of study is that of transcriptome changes that occur due to the stresses imposed throughout cryopreservation. Studies by Ren et al (2015) and Gross et al (2017) explored the regulation of transcripts in response to exposure to CPAs and LN on A. thaliana. Ren et al (2015) primarily looked at the effect of addition of vitamin C to PVS2, and its effect on genes related to ROS stresses.…”

“…Studies by Ren et al (2015) and Gross et al (2017) explored the regulation of transcripts in response to exposure to CPAs and LN on A. thaliana. Ren et al (2015) primarily looked at the effect of addition of vitamin C to PVS2, and its effect on genes related to ROS stresses. It was seen that, compared with controls, plant tissue incubated in PVS2 with vitamin C upregulated genes associated with antioxidant formation as well as genes associated with ROS-scavenging.…”

Single-wall Carbon Nanotubes Improve Cell Survival Rate and Reduce Oxidative Injury in Cryopreservation of Agapanthus praecox Embryogenic Callus

“…ROS-induced oxidative stress is the fundamental trigger of cell death during cryopreservation [8]. Ren et al [7,9] utilized comparative transcriptomics to obtain genes response to cryoinjury and revealed that peroxidation was a key element affecting viability, and genes related to oxidative stress played important roles in Arabidopsis thaliana cryopreservation. Zhang et al [10] revealed that oxidative stress and apoptosis were the major factors which injure embryogenic callus during Agapanthus praecox cryopreservation.…”

Exogenous ascorbic acid delayed leaf senescence of early flowering rice mutant FTL10

“…AsA belongs to water-soluble antioxidants and is one of the most important vitamins widely distributed in various plants (Guerreiro et al 2010). Some previous studies have demonstrated that AsA plays important roles in the regulation of mitosis and cell expansion (Smirnoff and Wheeler 2000), in electron transport chain (Tóth et al 2009), and particularly, in scavenging of ROS (Ren et al 2015). AsA is an important antioxidant of the ROS scavenging mechanism; it helps to reduce peroxides (Potters et al 2010).…”