Peroxidase and Polyphenol Oxidase Activity in Moderate Resistant and Susceptible Vicia faba Induced by Aphis craccivora (Hemiptera: Aphididae) Infestation

Soffan, Alan; Alghamdi, Salem S.; Aldawood, Abdulrahman S.

doi:10.1093/jisesa/ieu147

Cited by 23 publications

(17 citation statements)

References 45 publications

(60 reference statements)

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“…On the one hand, they are involved in the efficient removal of ROS (Gulsen et al , 2010), on the other, in generation of highly reactive quinones, which are toxic for insects (Shivashankar et al , 2012). The increase of POD activity due to insect feeding has been recorded in different plant species (Gailite et al , 2005; Taggar et al , 2012; Golan et al , 2013; Kot et al , 2015) and can be used with POD as a biochemical marker of resistant cultivars (Wei et al , 2007; Gulsen et al , 2010; Soffan et al , 2014). Enhance POD activity indicates that the stress was induced and the secondary metabolites were released as a defensive mechanism (Biswas et al , 2014).…”

Section: Discussionmentioning

confidence: 99%

Biochemical responses induced in galls of three Cynipidae species in oak trees

Kot

Jakubczyk

Karaś

et al. 2017

Bull. Entomol. Res.

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Gall-making Cynipidae manipulate the leaves of host plant to form galls where offspring find shelter and food. The relationship between oak gallwasp and biochemical mechanisms of galls still requires a better understanding. So, in this research, protein and phenolic compound contents, as well as the activity of antioxidative enzymes and pathogenesis-related (PR) proteins were determined. Galls caused by asexual generation of Cynips quercusfolii L., Neuroterus numismalis (Fourc.) and N. quercusbaccarum L., as a model were used. All cynipid species modified the protein levels of gall tissues, but they cannot be treated as protein sinks. Significantly higher levels of phenols were observed in the galled leaves and galls of all cynipid species when compared with the control tissues. Peroxidase and polyphenol oxidase activity was usually low or showed no activity in galled tissues of all species. PR proteins, such as chitinase and β-1,3-glucanase, had a similar activity profile. Their activity significantly increased in the leaves with galls of all cynipid species, especially those infested with C. quercusfolii. Data generated in this study clearly indicate that galling Cynipidae manipulate the biochemical machinery of the galls for their own needs. However, the pattern of the biochemical features of leaves with galls and galled tissues depends on gall-making species.

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Section: Discussionmentioning

confidence: 99%

Biochemical responses induced in galls of three Cynipidae species in oak trees

Kot

Jakubczyk

Karaś

et al. 2017

Bull. Entomol. Res.

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“…Upregulation of other genes at day 1 involved peroxidases, cytochrome P450s. The role of peroxidases in scavenging ROS during the defense mechanism has been clearly documented in plant-aphid interactions [77,78] including soybean-soybean aphid interaction [18]. Plant cytochrome P450s are importantly involved in jasmonic acid mediated plant defense in response to wound and insect attack [79].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Induced Susceptibility Effects of Soybean Aphid on Soybean

Neupane

Varenhorst

Nepal

2020

Preprint

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Soybean aphid (SBA) is one of the major pests of soybean (Glycine max) in the United States of America. The main objective of this research was to characterize interactions between two different biotypes of soybean aphids in susceptible and resistant soybean cultivars. Demographic and transcriptomic responses of susceptible and resistant (Rag1) soybean cultivars to aphid feeding were investigated in soybean plants colonized by aphids (biotype 1) in the presence or absence of inducer population (biotype 2) at day 1 and day 11. Leaves tissues collected at day 1 and day 11 post infestation were used for RNA sequencing, and ten RNA datasets with 266,535,654 sequence reads were analyzed. In the presence of inducer population, we found 746 and 243 differentially expressed genes (DEGs) in susceptible and resistant cultivars, respectively at day 1, whereas 981 and 377 DEGs were found in susceptible and resistant cultivars, respectively at day 11. Enrichment analysis showed a response to chitin, lignin catabolic and metabolic process, asparagine metabolic process, response to chemical unique to treatment with no inducer population, whereas, response to reactive oxygen species, photosynthesis, regulation of endopeptidase activity unique to treatment with inducer population. Furthermore, 14 DEGs were observed in Rag QTLs regions, particularly six DEGs in Rag1 containing QTL. The identified DEGs in the experiment in both resistant and susceptible cultivars during the interaction of soybean and SBA are potential candidates for furthering investigation into induced susceptibility.

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“…Finally, the chromogenic agent was added and statically for 10 min. The results were determined at 550nm [14] .…”

Section: Determination Of Peroxidase (Pod) Content Under Different Stmentioning

confidence: 99%

The study on the mechanism of “oil-spilling” of wolfberry (Lycium barbarum)fruits during storage

Wang

Xiong

et al. 2018

Preprint

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Improper storage of wolfberry fruit is liable to soften, discoloration and adhesion, we call it "oil-spilling". The purpose of the study is to explore the mechanism of "oil-spilling" of wolfberry fruits during storage. In our study, the relationship between water content, superoxide dismutase (SOD), peroxidase (POD) activity, malondialdehyde (MDA) content and the cross section, epidermal cells of wolfberry fruit were studied during storage. Results showed that wolfberry fruits began to "oil-spilling" on the 20th day, and with the extension of storage time, the degree of "oil-spilling" gradually deepened, the content of water increased and the activity of POD decreased, and the content of MDA increased. It suggested that "oilspilling" was the result of improper external storage conditions, which it could promote the increase of water content and reactive oxygen free radical of wolfberry fruit, and lead to damage of cell structure and function and lipid peroxidation of cell membrane. The results explain the mechanism of "oil-spilling" and provide a strong basis for the establishment of scientific storage methods of wolfberry fruit.

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Peroxidase and Polyphenol Oxidase Activity in Moderate Resistant and Susceptible Vicia faba Induced by Aphis craccivora (Hemiptera: Aphididae) Infestation

Cited by 23 publications

References 45 publications

Biochemical responses induced in galls of three Cynipidae species in oak trees

Biochemical responses induced in galls of three Cynipidae species in oak trees

Characterization of Induced Susceptibility Effects of Soybean Aphid on Soybean

The study on the mechanism of “oil-spilling” of wolfberry (Lycium barbarum)fruits during storage

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