Perivascular multi-lineage progenitor cells in human organs: Regenerative units, cytokine sources or both?

Chen, Chien-Wen; Montelatici, Elisa; Crisan, Mihaela; Corselli, Mirko; Huard, Johnny; Lazzari, Lorenza; Péault, Bruno

doi:10.1016/j.cytogfr.2009.10.014

Cited by 146 publications

(133 citation statements)

References 49 publications

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“…56 These observations must be confirmed within multiple other cell lineages before a generalized role of perivascular cells in tissue homeostasis can be concluded. Nevertheless, as previously illustrated, perivascular cells are strong candidates for medical tissue regeneration, even before expansion in culture into MSC-like 72 and are, therefore, candidate trophic "drugstore" cells. 73 …”

Section: Discussionmentioning

confidence: 99%

Perivascular Ancestors of Adult Multipotent Stem Cells

Corselli

Chen

Crisan

et al. 2010

ATVB

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Abstract-Independent studies by numerous investigators have shown that it is possible to harvest multipotent progenitor cells from diverse dissociated and cultured fetal, perinatal, and principally adult developed tissues. Despite the increasingly recognized medical value of these progenitor cells, the archetype of which remains the mesenchymal stem cell, this indirect extraction method has precluded the understanding of their native identity, tissue distribution, and frequency. Consistent with other researchers, we have hypothesized that blood vessels in virtually all organs harbor ubiquitous stem cells. We have identified, marked, and sorted to homogeneity by flow cytometry endothelial and perivascular cells in a large selection of human fetal, perinatal, and adult organs. Perivascular cells, including pericytes in the smallest blood vessels and adventitial cells around larger ones, natively express mesenchymal stem cell markers and produce in culture a long-lasting progeny of multilineage mesodermal progenitor cells.

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Section: Discussionmentioning

confidence: 99%

Perivascular Ancestors of Adult Multipotent Stem Cells

Corselli

Chen

Crisan

et al. 2010

ATVB

Self Cite

287

209

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“…The determination of the origin and identity of SCs together with their niches in adult tissue also provides important information on their participation in endogenous tissue regeneration and their possible applications as pluri-or multipotent cells in cellular regeneration therapy (6,7,(27)(28)(29)(30)(31)(32)(33). Evidence accumulated in the last few years show that the adult macro-and microvessels contain multi-or pluripotent SCs, including MSCs, and/or pericytes, as well as hemopoietic SCs, and lineage committed progenitors, such as vascular walls endothelial progenitor and Sca-1 1 smooth muscle progenitor cells (6,7,23,(29)(30)(31)(33)(34)(35)(36). Pericyte SCs have been isolated from different vascular tissues including abdominal adipose, bone marrow, dental pulp, and umbilical cord (6,7,23,(29)(30)(31)(33)(34)(35).…”

Section: Discussionmentioning

confidence: 99%

“…Evidence accumulated in the last few years show that the adult macro-and microvessels contain multi-or pluripotent SCs, including MSCs, and/or pericytes, as well as hemopoietic SCs, and lineage committed progenitors, such as vascular walls endothelial progenitor and Sca-1 1 smooth muscle progenitor cells (6,7,23,(29)(30)(31)(33)(34)(35)(36). Pericyte SCs have been isolated from different vascular tissues including abdominal adipose, bone marrow, dental pulp, and umbilical cord (6,7,23,(29)(30)(31)(33)(34)(35). Actual discussions of the role of pericytes as SCs propose that pericytes are a source for MSC (29).…”

Section: Discussionmentioning

confidence: 99%

Neural differentiation of rat aorta pericyte cells

et al. 2011

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Pericyte perivascular cells, believed to originate mesenchymal stem cells (MSC), are characterized by their capability to differentiate into various phenotypes and participate in tissue reconstruction of different organs, including the brain. We show that these cells can be induced to differentiation into neural-like phenotypes. For these studies, pericytes were obtained from aorta ex-plants of Sprague-Dawley rats and differentiated into neural cells following induction with trans retinoic acid (RA) in serum-free defined media or differentiation media containing nerve growth and brain-derived neuronal factor, B27, N2, and IBMX. When induced to differentiation with RA, cells express the pluripotency marker protein stage-specific embryonic antigen-1, neural-specific proteins b3-tubulin, neurofilament-200, and glial fibrillary acidic protein, suggesting that pericytes undergo differentiation, similar to that of neuroectodermal cells. Differentiated cells respond with intracellular calcium transients to membrane depolarization by KCl indicating the presence of voltage-gated ion channels and express functional Nmethyl-D-aspartate receptors, characteristic for functional neurons. The study of neural differentiation of pericytes contributes to the understanding of induction of neuroectodermal differentiation as well as providing a new possible stem-cell source for cell regeneration therapy in the brain. ' 2011 International Society for Advancement of Cytometry

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“…Our novel findings above on the perivascular behavior of DFAT cells were consistent to the new understandings of MSCs. Recent studies identified that pericytes in multiple organs and tissues could demonstrate perivascular markers (CD146, NG2 and CD140b) and MSCs markers (CD44, CD73, CD90, CD105), exhibiting osteogenic, chondrogenic and adipogenic potentials [55][56][57]. MSCs within adult mesenchymal tissues such as bone and adipose also could differentiate into or act as pericytes without induction of growth factors [58][59].…”

Section: Angiogenesismentioning

confidence: 99%