Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues

Shen, Jiefei; Sugawara, Atsunori; Yamashita, Joe; Ogura, Hideo; Sato, Soh

doi:10.4248/ijos11044

Cited by 85 publications

(80 citation statements)

References 54 publications

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“…Early studies suggest that DFAT cells have a partial stem cell signature less robust than ADSCs. 2 However, these studies reported on DFAT cells and ADSCs from early passages 3,4 and did not compare DFAT cells from the same patient and fat depot.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Markers and Functional Attributes of Human Adipose-Derived Stem Cells and Dedifferentiated Adipocyte Cells from Subcutaneous Fat of an Obese Diabetic Donor

Watson

Patel

Moor

et al. 2014

Advances in Wound Care

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Section: Introductionmentioning

confidence: 99%

Comparison of Markers and Functional Attributes of Human Adipose-Derived Stem Cells and Dedifferentiated Adipocyte Cells from Subcutaneous Fat of an Obese Diabetic Donor

Watson

Patel

Moor

et al. 2014

Advances in Wound Care

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“…Bioengineered human stromal cells, such as paired isogenic gene knock-in and knockout cells could be stably transduced with GFP, which will allow researchers to track them continuously in order to monitor their fate and geometrical contribution with time. With the help of 3D multiphoton microscopy, qRT-PCR and microarray techniques, researchers will be able to establish the tissue architecture, heterotypic cell-cell interactions, cell replication/differentiation, dedifferentiation/transdifferentiation, 107,108 and differential gene expression profiles. The establishment of efficient and reproducible 3D B/BM culture and stem cell expansion protocols suitable for industry and automated high throughput platforms will enable the development of time-saving strategies for clinical applications.…”

Section: Perspectivementioning

confidence: 99%

Three dimensionalde novomicro bone marrow and its versatile application in drug screening and regenerative medicine

Liu

et al. 2015

Exp Biol Med (Maywood)

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The finding that bone marrow hosts several types of multipotent stem cell has prompted extensive research aimed at regenerating organs and building models to elucidate the mechanisms of diseases. Conventional research depends on the use of two-dimensional (2D) bone marrow systems, which imposes several obstacles. The development of 3D bone marrow systems with appropriate molecules and materials however, is now showing promising results. In this review, we discuss the advantages of 3D bone marrow systems over 2D systems and then point out various factors that can enhance the 3D systems. The intensive research on 3D bone marrow systems has revealed multiple important clinical applications including disease modeling, drug screening, regenerative medicine, etc. We also discuss some possible future directions in the 3D bone marrow research field.

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“…In this protocol, floating unilocular mature adipocytes adhere to the top inner surface of a culture flask filled completely with medium. This cell fraction, known as dedifferentiated fat (DFAT) cells (Nobusue et al 2008), is abundant, homogenous, could be re-differentiated into mature adipocytes (Matsumoto et al 2008), and exhibits multilineage potential in vitro (Matsumoto et al 2008;Shen et al 2011). In our study, the supernatants showed closer functional properties to the reported DFAT cells than to the floating ones.…”

Section: Resultsmentioning

confidence: 99%

Adipogenic potential of stem cells derived from rabbit subcutaneous and visceral adipose tissue in vitro

Vachkova

Bosnakovski

Yonkova

et al. 2016

In Vitro Cell.Dev.Biol.-Animal

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Rabbits are considered as appropriate animal models to study some obesity-associated abnormalities because of the similarity of their blood lipid profile and metabolism to humans. The current study was focused on comparison of adipose differentiation ability in rabbit adipose-derived stem cells (ADSC) in vitro. Subcutaneous and visceral stromal vascular fractions (SVF) were isolated from three 28-d-old New Zealand rabbits by collagenase digestion. Supernatants from both isolates were collected 24 h after the initial plating. On the fourth passage, all isolated cell types undergo triplicate adipogenic induction. The adipose induction potential was calculated as percentage of increasing optical density (OD) values. The data revealed that with increasing the number of induction cycles, the induction tendency in visceral ADSC decreased in contrast to the subcutaneous ones. Although the supernatants did not reach induction levels of their relevant precursors, they follow the same pattern in both subcutaneous and visceral ADSC. All cell types successfully passed osteogenic and chondrogenic differentiation. In conclusion, the best adipose induction ability was observed in directly plated subcutaneous cell population. The increase of induction numbers depressed adipose induction ability in cell populations derived from visceral fat depots.

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Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues

Cited by 85 publications

References 54 publications

Comparison of Markers and Functional Attributes of Human Adipose-Derived Stem Cells and Dedifferentiated Adipocyte Cells from Subcutaneous Fat of an Obese Diabetic Donor

Comparison of Markers and Functional Attributes of Human Adipose-Derived Stem Cells and Dedifferentiated Adipocyte Cells from Subcutaneous Fat of an Obese Diabetic Donor

Three dimensionalde novomicro bone marrow and its versatile application in drug screening and regenerative medicine

Adipogenic potential of stem cells derived from rabbit subcutaneous and visceral adipose tissue in vitro

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