Periosteum Extracellular‐Matrix‐Mediated Acellular Mineralization during Bone Formation

Lin, Xianfeng; Zhao, Chenchen; Zhu, Peizhi; Chen, Jiaxin; Yu, Huaguang; Yin, Chang; Zhang, Qi; Qin, An; Fan, Shunwu

doi:10.1002/adhm.201700660

Cited by 50 publications

(28 citation statements)

References 41 publications

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“…Higher levels of mineralized tissue and increased vascular volume were observed 8 weeks after implantation, which might be caused by reduced production of IL-1b and IL-8 and superior osteogenic capacity compared to native GP (Cunniffe et al, 2017). In addition, 3D ECM scaffold produced from decellularized periosteum promoted bone mineralization by controlling the size and direction of mineral crystals in rabbit bone defect regeneration, suggesting the crucial role of periosteum ECM in efficient healing of fractures and bone regeneration (Lin et al, 2018). In clinical, decellularized bone ECM from bovine trabecular bone discs with patient autogenous MSCs could treat distal tibia fracture.…”

Section: Tissue-derived Decellularized Ecm Scaffoldmentioning

confidence: 96%

The Bone Extracellular Matrix in Bone Formation and Regeneration

et al. 2020

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Bone regeneration repairs bone tissue lost due to trauma, fractures, and tumors, or absent due to congenital disorders. The extracellular matrix (ECM) is an intricate dynamic bio-environment with precisely regulated mechanical and biochemical properties. In bone, ECMs are involved in regulating cell adhesion, proliferation, and responses to growth factors, differentiation, and ultimately, the functional characteristics of the mature bone. Bone ECM can induce the production of new bone by osteoblast-lineage cells, such as MSCs, osteoblasts, and osteocytes and the absorption of bone by osteoclasts. With the rapid development of bone regenerative medicine, the osteoinductive, osteoconductive, and osteogenic potential of ECM-based scaffolds has attracted increasing attention. ECM-based scaffolds for bone tissue engineering can be divided into two types, that is, ECM-modified biomaterial scaffold and decellularized ECM scaffold. Tissue engineering strategies that utilize the functional ECM are superior at guiding the formation of specific tissues at the implantation site. In this review, we provide an overview of the function of various types of bone ECMs in bone tissue and their regulation roles in the behaviors of osteoblast-lineage cells and osteoclasts. We also summarize the application of bone ECM in bone repair and regeneration. A better understanding of the role of bone ECM in guiding cellular behavior and tissue function is essential for its future applications in bone repair and regenerative medicine.

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Section: Tissue-derived Decellularized Ecm Scaffoldmentioning

confidence: 96%

The Bone Extracellular Matrix in Bone Formation and Regeneration

et al. 2020

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“…Increasing evidence showed that ECM-based TEB was capable of delivering biological signals, such as cell adhesion sites, which is analogous to bone regeneration microenvironments and a new strategy for tissue repair. 23–26 Previous studies conducted by our group have found that MSC ECM-based TEBs released a variety of active proteins with osteogenesis activity and increased osteoinductivity. In the present study, we demonstrated that MSC/POC ECM-based TEBs significantly enhanced osteogenesis in a rat femur defect model compared with MSC ECM-based TEBs.…”

Section: Discussionmentioning

confidence: 98%

Engineered scaffolds based on mesenchymal stem cells/preosteoclasts extracellular matrix promote bone regeneration

et al. 2020

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Recently, extracellular matrix-based tissue-engineered bone is a promising approach to repairing bone defects, and the seed cells are mostly mesenchymal stem cells. However, bone remodelling is a complex biological process, in which osteoclasts perform bone resorption and osteoblasts dominate bone formation. The interaction and coupling of these two kinds of cells is the key to bone repair. Therefore, the extracellular matrix secreted by the mesenchymal stem cells alone cannot mimic a complex bone regeneration microenvironment, and the addition of extracellular matrix by preosteoclasts may contribute as an effective strategy for bone regeneration. Here, we established the mesenchymal stem cell/preosteoclast extracellular matrix -based tissue-engineered bones and demonstrated that engineered-scaffolds based on mesenchymal stem cell/ preosteoclast extracellular matrix significantly enhanced osteogenesis in a 3 mm rat femur defect model compared with mesenchymal stem cell alone. The bioactive proteins released from the mesenchymal stem cell/ preosteoclast extracellular matrix based tissue-engineered bones also promoted the migration, adhesion, and osteogenic differentiation of mesenchymal stem cells in vitro. As for the mechanisms, the iTRAQ-labeled mass spectrometry was performed, and 608 differentially expressed proteins were found, including the IGFBP5 and CXCL12. Through in vitro studies, we proved that CXCL12 and IGFBP5 proteins, mainly released from the preosteoclasts, contributed to mesenchymal stem cells migration and osteogenic differentiation, respectively. Overall, our research, for the first time, introduce pre-osteoclast into the tissue engineering of bone and optimize the strategy of constructing extracellular matrix–based tissue-engineered bone using different cells to simulate the natural bone regeneration environment, which provides new sight for bone tissue engineering.

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“…Decellularized ECM derived from porcine periosteum has been shown to promote mineral deposition and orthotopic bone formation in rabbits. [290] Additionally, when administered in the form of a hydrogel, decellularized periosteal ECM can promote M2 macrophage polarization, angiogenesis, and mature bone formation in rat critical-sized calvarial defects. [291] However, when implanted into a calvarial defect, the decellularized periosteal ECM hydrogel is subject to relatively rapid degradation (<7 days).…”

Section: Decellularized Ecmmentioning

confidence: 99%

“…Due to the critical role of periosteum in the bone repair process, a number of research groups have attempted to utilize decellularized periosteal ECM for inducing the repair of bone defects. Decellularized ECM derived from porcine periosteum has been shown to promote mineral deposition and orthotopic bone formation in rabbits 290. Additionally, when administered in the form of a hydrogel, decellularized periosteal ECM can promote M2 macrophage polarization, angiogenesis, and mature bone formation in rat critical‐sized calvarial defects 291.…”

Section: Strategies To Improve Biomaterials For Bone Regenerationmentioning

confidence: 99%

Functional Biomaterials for Bone Regeneration: A Lesson in Complex Biology

Armiento

Hatt

Rosenberg

et al. 2020

Adv Funct Materials

152

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Bone is a hard yet dynamic tissue with remarkable healing capacities. Research to date has greatly advanced the understanding of how bone heals and has led to marked success in the treatment of bone injuries. Nevertheless, the effective treatment of nonunions and large bone defects continues to present a challenge for orthopedic surgeons. Biomaterials provide researchers with a powerful instrument to potentially guide effective bone tissue regeneration in challenging healing environments. However, the most appropriate biomaterial for bone tissue engineering continues to be an area of intense debate. Indeed, the mechanical properties of real bone can be reproduced in vitro but the development of a functional bone substitute goes beyond the sole mechanical properties. The faithful reproduction of bone as a functional organ requires the combination of different cell types and temporal regulation of the molecular signaling involved during the different stages of bone formation and regeneration. This is not at all a trivial task. Herein, critical aspects of bone healing/regeneration including mechanical loading, inflammation, vascularization, and innervation are described. The success and the challenges behind the development of biomaterials, and the technologies used to functionalize them, in order to support the underlying cellular mechanisms are also highlighted.

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Periosteum Extracellular‐Matrix‐Mediated Acellular Mineralization during Bone Formation

Cited by 50 publications

References 41 publications

The Bone Extracellular Matrix in Bone Formation and Regeneration

The Bone Extracellular Matrix in Bone Formation and Regeneration

Engineered scaffolds based on mesenchymal stem cells/preosteoclasts extracellular matrix promote bone regeneration

Functional Biomaterials for Bone Regeneration: A Lesson in Complex Biology

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