Periosteal Fibroblasts Synthesize a Cytokine That Stimulates Bone Resorption and Demonstrates Interleukin-1 Activity After Partial Purification

Heath, Joan K.; Saklatvala, Jeremy; Meikle, Murray C.; Atkinson, Susan J.; Reynolds, JJ

doi:10.1093/rheumatology/xxiv.suppl_1.136

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Cytokines1

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2001

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Cited by 6 publications

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“…In humans, two distinct interleukin‐1 gene products have been cloned interleukin‐1α and interleukin‐1β. Sources of interleukin‐1 production includes mononuclear phagocytes, keratinocytes (185), fibroblasts (125), endothelial cells (203) and osteoblasts (119). Interleukin‐1 is a major mediator in periodontitis (234, 288).…”

Section: Cytokinesmentioning

confidence: 99%

Etiopathogenesis of periodontitis in children and adolescents

Kinane¹,

Podmore²,

Ebersole³

2001

Periodontology 2000

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Section: Cytokinesmentioning

confidence: 99%

Etiopathogenesis of periodontitis in children and adolescents

Kinane¹,

Podmore²,

Ebersole³

2001

Periodontology 2000

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Stimulation of bone resorption and cell proliferation in vitro by human gingival fibroblasts from patients with periodontal disease

1990

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Effect of lipopolysaccharide from Porphyromonas gingivalis on prostaglandin E₂ and interleukin‐1β release from rat periosteal and human gingival fibroblasts in vitro

Sismey-Durrant

Hopps

1991

Oral Microbiology and Immunology

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Lipopolysaccharide (LPS) was extracted from Porphyromonas gingivalis (W83) by the Westphal procedure, nuclease-digested and ultracentrifuged. Fibroblasts were obtained from human gingival tissue and rat periosteum, grown to confluence then stimulated in serum-free medium with 0.1, 1.0 and 10.0 micrograms/ml LPS. The levels of prostaglandin E2 (PGE2) and interleukin-1 beta (IL-1 beta) released were measured after 2, 4 and 6 d by specific radioimmunoassays. Unstimulated gingival fibroblasts produced low levels of PGE2 (24.5 +/- 1.5 (SD) ng/ml) and IL-1 beta (0.34 +/- 0.29 ng/ml). LPS stimulated statistically significant dose-related increases in PGE2 and IL-1 beta at the concentrations of LPS tested. At 10.0 micrograms/ml, LPS-stimulated fibroblasts produced 363.5 +/- 40.3 ng/ml PGE2 and 1.81 +/- 0.1 ng/ml IL-1 beta in 6 d. These results demonstrate that LPS from P. gingivalis is capable of stimulating PGE2 and IL-1 beta release from fibroblasts. This would appear to be an additional mechanism by which LPS can induce tissue breakdown in periodontal disease.

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Periosteal Fibroblasts Synthesize a Cytokine That Stimulates Bone Resorption and Demonstrates Interleukin-1 Activity After Partial Purification

Cited by 6 publications

References 0 publications

Etiopathogenesis of periodontitis in children and adolescents

Etiopathogenesis of periodontitis in children and adolescents

Stimulation of bone resorption and cell proliferation in vitro by human gingival fibroblasts from patients with periodontal disease

Effect of lipopolysaccharide from Porphyromonas gingivalis on prostaglandin E₂ and interleukin‐1β release from rat periosteal and human gingival fibroblasts in vitro

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Periosteal Fibroblasts Synthesize a Cytokine That Stimulates Bone Resorption and Demonstrates Interleukin-1 Activity After Partial Purification

Cited by 6 publications

References 0 publications

Etiopathogenesis of periodontitis in children and adolescents

Etiopathogenesis of periodontitis in children and adolescents

Stimulation of bone resorption and cell proliferation in vitro by human gingival fibroblasts from patients with periodontal disease

Effect of lipopolysaccharide from Porphyromonas gingivalis on prostaglandin E2 and interleukin‐1β release from rat periosteal and human gingival fibroblasts in vitro

Contact Info

Product

Resources

About

Effect of lipopolysaccharide from Porphyromonas gingivalis on prostaglandin E₂ and interleukin‐1β release from rat periosteal and human gingival fibroblasts in vitro