Stimulation of bone resorption and cell proliferation in vitro by human gingival fibroblasts from patients with periodontal disease

Lerner, Ulf H.; Hänström, Lennart; Sjöström, Staffan

doi:10.1016/0169-6009(90)90264-g

Cited by 8 publications

(8 citation statements)

References 32 publications

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“…Con®rming previous reports from our own laboratory (10,11), as well as a report by Cochran and Rouse (24), we show here that conditioned media from human gingival ®broblasts isolated from patients with periodontal disease release factor(s) which stimulate bone resorption in vitro. We also found that IL-1a and IL-1b stimulated bone resorption in our mouse calvarial bioassay to the same degree as the HFCMs.…”

Section: Discussionsupporting

confidence: 91%

“…The possible importance of prostanoids in the 45 Ca release response induced by human gingival fibroblast conditioned media or interleukin-1 HFCM from 3 dierent patients (nos 7,11,12) were extensively ultra®ltrated against a ®lter with an assigned molecular weight cuto of 10,000 (YM 10). The concentrations of PGE 2 in these ultra®ltrated supernatants were 0.5±13 pg/ml.…”

Section: Resultsmentioning

confidence: 99%

“…In the in¯amed joint capsule not only leucocytes, but also constitutive synovial cells can secrete factors, including cytokines, matrix metallo-proteinases, plasminogen activators and prostaglandins, which can activate chondrocytic breakdown of cartilage and osteoclastic resorption of bone (7±9). Based upon these observations we have investigated whether gingival ®broblasts, the main constitutive cell in the gingiva, may secrete factors that can stimulate bone resorption (10,11). These studies are also prompted by our own observation, as well as reports by others (12) demonstrating that in¯ammatory cells in the in¯amed gingiva often are not lying close to the bone tissue, but are rather separated from the bone cells by a ®broblastic, non-in¯amed, cell layer.…”

mentioning

confidence: 91%

“…These studies are also prompted by our own observation, as well as reports by others (12) demonstrating that in¯ammatory cells in the in¯amed gingiva often are not lying close to the bone tissue, but are rather separated from the bone cells by a ®broblastic, non-in¯amed, cell layer. That such cells also may play a role in the loss of bone associated with periodontitis is suggested by our ®ndings showing that gingival ®broblasts, isolated from patients with periodontitis, can secrete one or several factors, which can stimulate bone resorption and cell proliferation in vitro (10,11). Since IL-1 is by far the most potent bone resorbing cytokine, and since IL-1 has been detected in gingivitis tissue and in the gingival crevicular¯uid from patients with periodontitis (13±15), in the present communication we have evaluated whether the bone resorbing factor released from the gingival ®broblasts may be IL-1.…”

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confidence: 98%

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The bone resorbing activity released by gingival fibroblasts isolated from patients with periodontitis is independent of interleukin‐1

Sjöström

Hänström

Lerner³

2000

J of Periodontal Research

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Supernatants from gingival fibroblast cultures obtained from 14 patients with periodontal disease contained factor(s) capable of stimulating bone resorption in vitro, as assessed by the release of 45Ca from neonatal mouse calvariae. The possibility that the factor(s) was interleukin-1 alpha (IL-1 alpha), IL-1 beta or prostaglandin E2 (PGE2) was next investigated. The human fibroblast conditioned media (HFCM) stimulated PGE2 biosynthesis in bone. The stimulatory effect by HFCM on 45Ca release, however, was not affected by blocking prostaglandin biosynthesis with indomethacin. In contrast, 45Ca release induced by IL-1 alpha, IL-1 beta, thrombin and bradykinin was significantly reduced by indomethacin, whereas the effects of PTH and PTHrP were unaffected by indomethacin. The concentration of PGE2 in HFCM was too low to be solely responsible for the 45Ca release response. In addition, the amount of bone resorbing activity produced by the gingival fibroblasts was unaffected by cyclo-oxygenase inhibitors. Similar to IL-1 alpha and IL-1 beta, the stimulatory effect of HFCM was inhibited by gamma-interferon. HFCM did not stimulate cyclic AMP formation in the mouse calvarial bones. Antisera which specifically blocked human IL-1 alpha or IL-1 beta induced 45Ca release, and the specific IL-1 receptor antagonistic protein, did not inhibit the stimulatory effect of HFCM. These data show that gingival fibroblasts secrete bone resorbing factor(s) which is not due to IL-1 and which stimulates bone resorption by a prostaglandin- and cyclic AMP-independent mechanism.

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Section: Discussionsupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 91%

mentioning

confidence: 98%

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The bone resorbing activity released by gingival fibroblasts isolated from patients with periodontitis is independent of interleukin‐1

Sjöström

Hänström

Lerner³

2000

J of Periodontal Research

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“…LPS is a potent stimulator of inflammatory cytokine production and bone resorption [6]. LPS induces inflammatory responses that target human gingival fibroblasts (HGF) [7].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the transduction pathway involved in c-fos and c-jun expression induced by Aggregatibacter actinomycetemcomitans lipopolysaccharides in human gingival fibroblasts

Gutiérrez-Venegas

Castillo-Alemán

2008

International Immunopharmacology

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In vitro studies on bone resorption in neonatal mouse calvariae using a modified dissection technique giving four samples of bone from each calvaria

Ljunggren

Ransjö

Lerner

1991

Journal of Bone and Mineral Research

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Bone resorption in a modified bone culture system, based on incubation of small fragments from neonatal mouse calvarial bones, has been studied. Four bone fragments were dissected out from each mouse calvaria and were thereafter cultured in CMRL 1066 medium in plastic multiwell dishes. Bone resorption was assessed by 45Ca release from prelabeled bones. The rate of bone resorption in response to parathyroid hormone (PTH) was less in the anterior part of the calvaria compared to the posterior part. After removing the anterior region, four parietal bone fragments that showed identical basal and PTH-stimulated release of 45Ca could be dissected out from each mouse. Excretion of lactate dehydrogenase and beta-glucuronidase was the same in bones cultured submerged or on grids. Uptake of [3H]thymidine in bones cultured submerged was 54% of [3H]thymidine uptake in bones cultured on grids. Dose-response curves, established by using parietal bone fragments, showed that the sensitivity and the magnitude of the increase in 45Ca release seen after stimulation with PTH, prostaglandin E2, and 1 alpha-hydroxyvitamin D3 were the same for bones cultured submerged or on grids. The 45Ca release in response to stimulation with PTH, prostaglandin E2, and 1 alpha-OHD3 was the same in calvarial fragments cultured submerged and those previously obtained with calvarial halves cultured on grids. Thus, even though the rate of DNA synthesis was slower in bones cultured submerged, the rate and the magnitude of resorption were the same in bones cultured on grids or submerged. These data show that it is possible to perform studies on bone resorption with small fragments of neonatal mouse parietal bones.

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Stimulation of bone resorption and cell proliferation in vitro by human gingival fibroblasts from patients with periodontal disease

Cited by 8 publications

References 32 publications

The bone resorbing activity released by gingival fibroblasts isolated from patients with periodontitis is independent of interleukin‐1

The bone resorbing activity released by gingival fibroblasts isolated from patients with periodontitis is independent of interleukin‐1

Characterization of the transduction pathway involved in c-fos and c-jun expression induced by Aggregatibacter actinomycetemcomitans lipopolysaccharides in human gingival fibroblasts

In vitro studies on bone resorption in neonatal mouse calvariae using a modified dissection technique giving four samples of bone from each calvaria

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