Perinatal Glyphosate-Based Herbicide Exposure in Rats Alters Brain Antioxidant Status, Glutamate and Acetylcholine Metabolism and Affects Recognition Memory

Gallegos, Cristina Eugenia; Baier, Carlos Javier; Bartos, Mariana; Bras, Cristina; Domínguez, Sergio; Mónaco, Nina; Gumilar, Fernanda; Giménez, María Sofía; Minetti, Alejandra

doi:10.1007/s12640-018-9894-2

Cited by 62 publications

(32 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly, the litter characteristics such as length, weight, sex ratio were not altered by the treatment. This observation is in agreement with recent studies investigating dam weight during pregnancy and litter characteristics following exposure to relatively low dose of GBH. Only higher doses were found to reduce weight with respect to juvenile or pregnant rat (500 and 1000 mg kg ‐1 BW d ‐1 ) peripartum exposure in dams .…”

Section: Discussionsupporting

confidence: 93%

“…It should be noted that the nucleus accumbens is a fundamental brain region involved in the motivation aspect of maternal behaviour and modulation of activity within this brain region only can alter proper maternal care toward the offspring . In addition to monoamines, acetylcholine esterase, which is the enzyme responsible for the breakdown of acetylcholine in the synaptic cleft to terminate synaptic transmission, is inhibited in the prefrontal cortex, striatum and hippocampus of male and female adult offspring after a perinatal exposure to Glyphosate . The extracellular glutamate concentration and the reuptake of amino acids are also hindered in the hippocampus following exposure to Glyphosate‐based herbicide …”

Section: Discussionmentioning

confidence: 99%

“…It should be added that the ventral and dorsal region of the hippocampus present physiological and functional differences, with the agreed consensus that the dorsal hippocampus is involved in spatial cognition and more cognitive performance and memory in general, whereas the ventral part is involved in the regulation of emotions, including stress and anxiety . Previous data suggest the GBH exposure affects hippocampal physiology and plasticity . To our knowledge, there are no data available on how glyphosate could affect maternal behaviour and the associated neuroplasticity.…”

Section: Introductionmentioning

confidence: 99%

“…Although it is hypothesised that the effects of glyphosate in the brain might be linked to glutamate excitotoxicity and oxidative stress, the molecular target(s) of glyphosate in the brain is/are not well defined. As introduced above, glyphosate is best known for its direct inhibitory effects on EPSPS.…”

Section: Introductionmentioning

confidence: 99%

“…35,36 Previous data suggest the GBH exposure affects hippocampal physiology and plasticity. 22,26,[37][38][39] To our knowledge, there are no data available on how glyphosate could affect maternal behaviour and the associated neuroplasticity.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Glyphosate and glyphosate‐based herbicide exposure during the peripartum period affects maternal brain plasticity, maternal behaviour and microbiome

Dechartres

Pawluski

Guéguen

et al. 2019

J Neuroendocrinology

View full text Add to dashboard Cite

Glyphosate is found in a large array of non‐selective herbicides such as Roundup® (Monsanto, Creve Coeur, MO, USA) and is by far the most widely used herbicide. Recent work in rodent models suggests that glyphosate‐based herbicides during development can affect neuronal communication and result in altered behaviours, albeit through undefined mechanisms of action. To our knowledge, no study has investigated the effects glyphosate or its formulation in herbicide on maternal behaviour and physiology. In the present study, relatively low doses of glyphosate (5 mg kg‐1 d‐1), Roundup® (5 mg kg‐1 d‐1 glyphosate equivalent), or vehicle were administered by ingestion to Sprague‐Dawley rats from gestational day (GD) 10 to postpartum day (PD)22. The treatments significantly altered licking behaviour toward pups between PD2 and PD6. We also show in the dams at PD22 that Roundup exposure affected the maturation of doublecortin‐immunoreactive new neurones in the dorsal dentate gyrus of the hippocampus of the mother. In addition, the expression of synaptophysin was up‐regulated by glyphosate in the dorsal and ventral dentate gyrus and CA3 regions of the hippocampus, and down‐regulated in the cingulate gyrus. Although a direct effect of glyphosate alone or its formulation on the central nervous system is currently not clear, we show that gut microbiota is significantly altered by the exposure to the pesticides, with significant alteration of the phyla Bacteroidetes and Firmicutes. This is the first study to provide evidence that glyphosate alone or in formulation (Roundup) differentially affects maternal behaviour and modulates neuroplasticity and gut microbiota in the mother.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Glyphosate and glyphosate‐based herbicide exposure during the peripartum period affects maternal brain plasticity, maternal behaviour and microbiome

Dechartres

Pawluski

Guéguen

et al. 2019

J Neuroendocrinology

View full text Add to dashboard Cite

show abstract

Behavioral and neuroinflammatory changes caused by glyphosate: Base herbicide in mice offspring

Carneiro

Chaves

Neves

et al. 2022

Birth Defects Research

View full text Add to dashboard Cite

Background: Glyphosate is a pesticide considered of low toxicity, but scientific evidences show it can be harmful to health. This study aimed to evaluate the toxicity in mice offspring exposed to glyphosate-based herbicide (GBH) during the intrauterine period. Methods: Female matrices received glyphosate 0.3 mg/kg daily per oral throughout the gestational period, which was variable between 18 and 22 days.From the 25th until the 28th days post-birth, mice offspring were subjected to behavioral tests, and the prefrontal cortex was processed for immunohistochemical analysis. Results: Two significant behavioral changes were observed: anxiety in the GLIF0.3 group, increase in the behavior burying marbles in the marbleburying test and hyperactivity, expressed by the significant increase of the crossing number in the open field test. The increased microglia, TNF-alpha, and astrocyte expression were also observed in the prefrontal cortex of offspring treated with GLIF0.3. Conclusion:Exposure to GBH during mice intrauterine development induces hyperactive and anxious behavior, evidencing neuroinflammation.

show abstract

Perinatal exposure to glyphosate‐based herbicides induced neurodevelopmental behaviors impairments and increased oxidative stress in the prefrontal cortex and hippocampus in offspring

Oliveira

Rojas

Sá

et al. 2022

Intl J of Devlp Neuroscience

View full text Add to dashboard Cite

Glyphosate is the organophosphate pesticide most widely used in the world. Recent studies correlate exposure to glyphosate and the emergence of neurodevelopmental disorders. Therefore, it was objective to propose a rat model of perinatal exposure to glyphosate-based herbicides (GBH) to study associated neurodevelopmental disorders. Behavioral aspects and brain pathways were assessed in the prepubertal phase. For this, maternal treatment occurred throughout the entire gestation period (from GD0) until weaning on postnatal day 22 (PND 22). Control group received oral gavage with 5 mL/kg of saline per day and GBH group received oral gavage with 50 mg/kg of GBH per day (n = 10 per group). Maternal behavior was evaluated in PND 2-6. Offspring were evaluated for quantification of ultrasonic vocalizations (PND 5); homing behavior test (PND 13); and hole board, social play behavior, open field, and object recognition tests . Prefrontal cortex and hippocampus of the offspring were processed to evaluate oxidative stress. Maternal exposure to GBH impaired early social communication, olfactory discrimination, social play behavior, and the exploration of objects, in addition to increasing repetitive and stereotyped movements. GBH also increased oxidative stress. Therefore, perinatal GBH exposure induced behavioral and oxidative stress impairments in rats associated with neurodevelopmental disorders. The manifestations found in the offspring are in accordance with symptoms of autism spectrum disorder.

show abstract

Perinatal Glyphosate-Based Herbicide Exposure in Rats Alters Brain Antioxidant Status, Glutamate and Acetylcholine Metabolism and Affects Recognition Memory

Cited by 62 publications

References 78 publications

Glyphosate and glyphosate‐based herbicide exposure during the peripartum period affects maternal brain plasticity, maternal behaviour and microbiome

Glyphosate and glyphosate‐based herbicide exposure during the peripartum period affects maternal brain plasticity, maternal behaviour and microbiome

Behavioral and neuroinflammatory changes caused by glyphosate: Base herbicide in mice offspring

Perinatal exposure to glyphosate‐based herbicides induced neurodevelopmental behaviors impairments and increased oxidative stress in the prefrontal cortex and hippocampus in offspring

Contact Info

Product

Resources

About