Behavioral and neuroinflammatory changes caused by glyphosate: Base herbicide in mice offspring

Abstract

Background: Glyphosate is a pesticide considered of low toxicity, but scientific evidences show it can be harmful to health. This study aimed to evaluate the toxicity in mice offspring exposed to glyphosate-based herbicide (GBH) during the intrauterine period. Methods: Female matrices received glyphosate 0.3 mg/kg daily per oral throughout the gestational period, which was variable between 18 and 22 days.From the 25th until the 28th days post-birth, mice offspring were subjected to behavioral tests, and the prefrontal cortex was processed for immunohistochemical analysis. Results: Two significant behavioral changes were observed: anxiety in the GLIF0.3 group, increase in the behavior burying marbles in the marbleburying test and hyperactivity, expressed by the significant increase of the crossing number in the open field test. The increased microglia, TNF-alpha, and astrocyte expression were also observed in the prefrontal cortex of offspring treated with GLIF0.3. Conclusion:Exposure to GBH during mice intrauterine development induces hyperactive and anxious behavior, evidencing neuroinflammation.

Maternal pre-conceptional glyphosate exposure impacts the offspring hepatic and ovarian proteome

“…Maternal exposure to GLY or GBH has reported impacts on cardiac parameters ( de Marins et al , 2023 ), anxiety and hyperactivity ( de Castro Vieira Carneiro et al , 2023 ), immune responsivity ( Buchenauer et al , 2022 ), hepatic function ( Rieg et al , 2022 ; Teleken et al , 2020 ), and muscle structure ( Barbosa et al , 2022 ) in offspring and although the species and route of exposure differ, the potential for maternal exposure to impair offspring viability is supported. This study investigated a maternal pre-conceptional exposure to GLY at a dose not previously determined to be of concern for development and reproduction ( Ganesan and Keating, 2020 ; Ganesan et al , 2020 ; USEPA, 1993 ).…”