Perhitungan jumlah bakteri di Laboratorium Mikrobiologi menggunakan pengembangan metode Spektrofotometri

Rosmania, Rosmania; Yanti, Fitri

doi:10.56064/jps.v22i2.564

Cited by 15 publications

(17 citation statements)

References 9 publications

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“…These results prove that the extract ethanol melinjo leaves at doses of 300 and 600 mg/kgBW has almost the same activity to cure diarrhea as the positive control group. The results of the study are in line with the research study that there was a decrease in body weight of rats when infected with pathogenic bacteria [13].…”

Section: Changes In Rat Body Weightsupporting

confidence: 88%

“…Measurement of the absorbance value of the test run and the absorbance value of the McFarland 0.5 standard solution was tested by UV-Vis spectrophotometry at a wavelength of 625 nm. The result of the absorbance value of the test must be close to the absorbance value of the McFarland solution of 0.5 so that it is at the bacterial density of 1.5 x 10 8 CFU/ml [13].…”

Section: Determination Of the Density Of The Number Of Bacteria Esche...mentioning

confidence: 90%

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Antidiarrheal Activity of Extract Ethanol Melinjo Leaves (Gnetum Gnemon L. (Linn.)) in Wistar Male White Rats Induced by Escherichia Coli and Extract Standardization

Herlina

SOLIHAH²,

WIJAYA³

et al. 2022

Int J App Pharm

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Objective: Determination of the antidiarrheal activity of extract ethanol melinjo leaves (Gnetum gnemon L. (Linn.)) in Wistar male white rats induced by Escherichia coli and extract standardization has been performed. Methods: This research was conducted by in vivo method using white male rats of Wistar strain induced by Escherichia coli. The treatment group was divided into 6 groups: normal, negative control, positive control, and groups with doses 150, 300, and 600 mg/kg BW. Negative control was given Na-CMC and positive control Gentamicin. The initial time diarrhea occurred 24-30 h after administration of Escherichia coli suspension. Results: The results of the phytochemical screening of the ethanolic extract of melinjo contain secondary metabolites of flavonoids, steroids, tannins, and saponins. Standardization of the extract ethanol melinjo leaves (Gnetum gnemon L.) meet the predetermined standards, while the acid insoluble ash content parameters did not meet the predetermined standards. The parameters feces weight, feces consistency, frequency diarrhea, body weight, number of Escherichia coli colonies feces dose 600 mg/kg BW had an effect that was almost close to positive control. The results showed that anti-diarrheal effect dose 150 mg/kg BW 35.75%, dose 300 mg/kg BW 43.02%, dose 600 mg/kg BW 50.14%. This shows that ethanol extract melinjo leaves dose 600 mg/kg BW wasn’t significantly different from the positive control (p<0.05). Effective Dose (ED50) Ethanol Extract Melinjo Leave as antidiarrheal was 578.2468 mg/kg BW. Conclusion: Extract ethanol melinjo leaves (Gnetum gnemon L. (Linn)) dose 600 mg/kg BW has the potential to be used as antidiarrheal and its extract meet the predetermined standards, except the acid insoluble ash content parameters did not meet the predetermined standards.

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Section: Changes In Rat Body Weightsupporting

confidence: 88%

Section: Determination Of the Density Of The Number Of Bacteria Esche...mentioning

confidence: 90%

Antidiarrheal Activity of Extract Ethanol Melinjo Leaves (Gnetum Gnemon L. (Linn.)) in Wistar Male White Rats Induced by Escherichia Coli and Extract Standardization

Herlina

SOLIHAH²,

WIJAYA³

et al. 2022

Int J App Pharm

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“…After that, the sample was dried on sterile filter paper Samples of stems that have been cut into pieces with a size of ± 4 × 1 cm are placed in a petri dish containing PDA isolation media to which chloramphenicol (50 mg/1) hour has been added. The fungi culture was placed at 25 for 2 × 24 h [ 66 , 69 , 70 ].…”

Section: Methodsmentioning

confidence: 99%

Endophytic microbiome of Boehmeria nivea and their antagonism against latent fungal pathogens in plants

et al. 2022

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Background Pathogenic microbes still become obstacles that can reduce the quality of plant growth, including ramie (Boehmeria nivea) plants. The study identified the microbiome and antagonistic interaction of the endophytic community from the B. nivea is necessary to improve the production of the ramie plant, especially ramie stem organs for fiber materials. Results Twenty isolates of endophytic microorganisms were obtained from the roots, stems, leaves, and flowers. They were identified using the Internal Transcribed Spacer (ITS) region of ribosomal (rDNA), and its morphotypes obtained 20 isolates, with a composition of 9 species of bacteria and 11 species of fungi. Besides that, the disease observations on ramie stems showed that four species of pathogenic fungi were identified as Fusarium solani isolate 3,248,941, Fusarium solani isolates colpat-359, Fusarium oxysporum isolate N-61–2, Clonostachys rosea strain B3042. The endophytic microorganism of ramie ability was tested to determine their potential to inhibit the growth of the pathogenic fungi based on the in-vivo antagonist test. The isolated bacteria were only able to inhibit the growth of F. solani, with the highest percentage of 54–55%. Three species of endophytic fungi, including Cladosporium tennissimum, Fusarium falciforme, and Penicillium citrinum, showed the best inhibition against the fungal pathogen Fusarium solani with the highest inhibitory presentation of 91–95%. Inhibitory interaction between the endophytic microbes and the ramie pathogens indicated the type of antibiosis, competition, and parasitism. Conclusion The results of this study succeeded in showing the potential antifungal by endophytic fungi from ramie against the pathogens of the plant itself. P. citrinum isolate MEBP0017 showed the highest inhibition against all the pathogens of the ramie.

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“…Uji antibakteri dibuat menjadi tiga konsentrasi yaitu 20%, 25%, dan 30%, pengujian untuk kontrol negatif yang digunakan adalah basis sabun sedangkan untuk kontrol positif yang digunakan sabun cuci tangan merk X. Suspensi yang telah dibuat dibandingkan tingkat kekeruhanya dengan larutan Mc Farland 0,5. Larutan Mc Farland digunakan sebagai referensi untuk menyesuaikan dengan kekeruhan bakteri suspensi (Rosmania, 2020)., Uji homogenitas dilakukan dengan tujuan untuk dapat melihat apakah data bervariasi homogen atau tidak. Hasil uji homogenitas menunjukkan bahwa data homogen karena nilai signifikan lebih dari 0,05.Uji normalitas dilakukan dengan tujuan untuk mendapatkan melihat apakah sebaran data pada ketiga konsentrasi sediaan tersebut normal karena nilai signifikan lebih dari 0,05.…”

Section: Uji Aktivitas Antibakteri `unclassified

Formulasi Sabun Cair Cuci Tangan dan Uji Aktivitas Bakteri Staphylococcus aureus dari Ekstrak Daun Kelor (Moringa oleifera Lam.)

Waris¹,

Yanti²,

Okzelia³

et al. 2023

sinteza

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Soap is what can be used to wash hands so as to avoid bacteria that can cause diseases such as acne and boils. One of the natural ingredients in making soap is Moringa leaves. Moringa leaves are one of the plants that have antibacterial properties, Staphylococcus aureus bacteria are bacteria that can cause infectious diseases such as acne and boils. The purpose of this study was to formulate hand washing liquid soap from Moringa leaf extract (Moringa oliefera Lam.), to evaluate the antibacterial activity of the preparation against Staphylococcus aureus. Moringa extract was obtained by maceration method. Furthermore, phytochemical screening was carried out, then formulated into soap preparations at concentrations of 20% (F1), 25% (F2), and 30% (F3). The preparations were evaluated and tested for activity against Staphylococcus aureus. Based on the results of the study, the yield of ethanol extract of Moringa leaves was 32.35% and contained secondary metabolites such as alkaloids, flavonoids, saponins and tannins. Moringa leaf ethanol extract soap formula met the requirements of all test parameters including pH test, irritation test, specific gravity test, and organoleptic test. The results of the antibacterial activity test gave an inhibition zone of 20.2 mm, 21.5 mm, and 23.2 mm, respectively. For F1, F2 and F3 against Staphylcoccus aureus.

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Perhitungan jumlah bakteri di Laboratorium Mikrobiologi menggunakan pengembangan metode Spektrofotometri

Cited by 15 publications

References 9 publications

Antidiarrheal Activity of Extract Ethanol Melinjo Leaves (Gnetum Gnemon L. (Linn.)) in Wistar Male White Rats Induced by Escherichia Coli and Extract Standardization

Antidiarrheal Activity of Extract Ethanol Melinjo Leaves (Gnetum Gnemon L. (Linn.)) in Wistar Male White Rats Induced by Escherichia Coli and Extract Standardization

Endophytic microbiome of Boehmeria nivea and their antagonism against latent fungal pathogens in plants

Formulasi Sabun Cair Cuci Tangan dan Uji Aktivitas Bakteri Staphylococcus aureus dari Ekstrak Daun Kelor (Moringa oleifera Lam.)

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