Performance of two real-time PCR assays for hepatitis B virus DNA detection and quantitation

Kania, Dramane; Ottomani, Laure; Méda, Nicolas; Peries, Marianne; Dujols, Pierre; Bolloré, Karine; Rénier, Wendy; Viljoen, Johannes; Ducos, J; Perre, Philippe Van de; Tuaillon, Édouard

doi:10.1016/j.jviromet.2014.01.015

Cited by 18 publications

(9 citation statements)

References 32 publications

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“…Most of these studies have included in-house real-time PCR for the detection of HBV DNA (Pas et al, 2000;Santos et al, 2014;Kania et al, 2014), and the limit of detection varied from 91 IU/mL to 2000 copies/mL. These assays presented good concordance with commercial methods for HBV DNA quantification.…”

Section: Discussionmentioning

confidence: 99%

Usefulness of in-house PCR methods for hepatitis B virus DNA detection

Portilho

Baptista

Silva

et al. 2015

Journal of Virological Methods

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Section: Discussionmentioning

confidence: 99%

Usefulness of in-house PCR methods for hepatitis B virus DNA detection

Portilho

Baptista

Silva

et al. 2015

Journal of Virological Methods

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“…The list of pathogens that can be detected by DNA/RNA-based technology is large, which includes: analysis for the detection of tuberculosis (e.g. Mycobacterium tuberculosis [83]), human papillomavirus (HPV), Chlamydia trachomatis and Neisseria gonorrhoeae [84][85][86][87], avian influenza virus and influenza A (H1N1) virus [88][89], human immunodeficiency virus (HIV) [90,91], measles [92,93], hepatitis A, B, and C [94][95][96][97], among other pathogenic agents.…”

Section: Pathogen Traceability In the Human Body Using Molecular Markersmentioning

confidence: 99%

Predicting Physical Features and Diseases by DNA Analysis: Current Advances and Future Challenges

Cerqueira¹,

Ramallo²,

Hünemeier³

et al. 2016

J Forensic Res

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The 'omics' era and its concomitant technological advances have brought great insight into genetics. One of the most promising fields within human genetics is the prediction of physical traits from analysis of genetic material. Besides the predictive potential of DNA, the traceability of pathogenic agents in the human body through molecular analysis is also a field to be further exploited. In this review, we aim to discuss specific aspects of phenotypic prediction by analysing DNA, with special emphasis on normal variation, and the application of a technology known as 'Forensic DNA Phenotyping' (FDP). We also suggest the term 'Phenotype Informative Markers' (PIMs) to designate any molecular markers responsible for normal or pathological human phenotypic variation. In addition, we raise some recommendations related to forensic genetics, the molecular diagnosis of human diseases, and the traceability of pathogens in the human body, giving special emphasis to the need for validation of these tests with strict protocols. Some relevant concerns about privacy, ethics, and legality of such predictions have also been discussed. Finally, we look at perspectives on the use of epigenetic tools, and quote some examples of what has been done in this specific field.

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“…Viral load is a relevant marker for diagnosis and clinical follow up, in relation to human immunodeficiency virus, hepatitis C virus, and hepatitis B virus. Viral load has been used to evaluate the treatment, to change therapy or to search for resistant viral variants and has been proposed as a biomarker to predict the outcome of infection (persistance or elimination) as is the case with Human Papillomavirus . Herpes Simplex Virus type 2 (HSV‐2) is a DNA virus ascribed to the Herpesviridae family, and the main etiological agent of genital ulcers.Viral quantification of HSV‐2 is used to study asymptomatic infections, monitoring neonatal and encephalitis herpes cases, as well as the risk of acquisition and transmission of HIV …”

Section: Introductionmentioning

confidence: 99%

“…Viral load has been used to evaluate the treatment, to change therapy or to search for resistant viral variants and has been proposed as a biomarker to predict the outcome of infection (persistance or elimination) as is the case with Human Papillomavirus. [1][2][3][4] Herpes Simplex Virus type 2 (HSV-2) is a DNA virus ascribed to the Herpesviridae family, and the main etiological agent of genital ulcers.Viral quantification of HSV-2 is used to study asymptomatic infections, monitoring neonatal and encephalitis herpes cases, as well as the risk of acquisition and transmission of HIV. [5][6][7][8] The quantitative real time PCR (qPCR) used to measure viral loads, because of its high sensitivity, can be applied to different biological samples such as serum, plasma, urine, genital secretions, and anal exudates.…”

Section: Introductionmentioning

confidence: 99%

Real time PCR to evaluate HSV‐2 shedding from anal and genital samples among men who have sex with men, living with HIV

Vergara-Ortega

Sevilla-Reyes

Herrera-Ortíz

et al. 2018

Journal of Medical Virology

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This study shows the relative quantification of HSV-2 by qPCR, using the MIQE Guidelines. The reaction efficiency was evaluated, and the relative quantification used the R = 2 method. The relative quantification of HSV-2 was conducted with anal and genital samples from men who have sex with men (MSM), living with HIV. The presence of a single amplification product was validated with a dissociation curves profile and the determination of the melting temperature. The limit of detection for β-globin was determined as 3.3 × 10 ng/μL, and for HSV-2 at 6.0 × 10 ng/μL. The efficiency for β-globin was 100.2% and for HSV-2 was 106.8%. From 336 MSM, 2.1% and 3.9% individuals presented anal or genital HSV-2 shedding, respectively. The HSV-2 viral load was 9.2 RU, individuals with fewer CD4 presented higher HSV-2 viral load. The qPCR method is reproducible and has optimal reaction efficiency.

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Performance of two real-time PCR assays for hepatitis B virus DNA detection and quantitation

Cited by 18 publications

References 32 publications

Usefulness of in-house PCR methods for hepatitis B virus DNA detection

Usefulness of in-house PCR methods for hepatitis B virus DNA detection

Predicting Physical Features and Diseases by DNA Analysis: Current Advances and Future Challenges

Real time PCR to evaluate HSV‐2 shedding from anal and genital samples among men who have sex with men, living with HIV

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