Real time PCR to evaluate HSV‐2 shedding from anal and genital samples among men who have sex with men, living with HIV

Vergara-Ortega, Dayana Nicté; Sevilla-Reyes, Edgar; Herrera-Ortíz, Antonia; Torres‐Ibarra, Leticia; Salmerón, Jorge; Lazcano‐Ponce, Eduardo; Sánchez-Alemán, Miguel Ángel

doi:10.1002/jmv.25003

Cited by 9 publications

(7 citation statements)

References 37 publications

(63 reference statements)

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“…GAPDH was used as internal reference for β-catenin and c-Myc, and the reaction conditions were: Pre-denaturation at 95°C for 30 sec, denaturation at 95°C for 5 sec, 60°C for 20 sec, for 40 cycles. Each experiment was repeated 3 times and the relative expression of the genes was detected by 2 −ΔCq method ( 11 ). Primer sequences are shown in Table I .…”

Section: Methodsmentioning

confidence: 99%

Effect of bortezomib on proliferation and apoptosis of myeloma cells by activating Wnt/β‑catenin signaling pathway

2020

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Effect of bortezomib on proliferation and apoptosis of myeloma cells by activating Wnt/β-catenin signaling pathway was investigated. Myeloma cells RPMI-8226 treated with different concentrations of bortezomib were used as experimental groups, and the untreated cells were used as the control group. The proliferation and apoptosis in all groups of cells were detected, as well as the expression levels of Wnt/β-catenin signaling pathway-related proteins, β-catenin and c-Myc. The results revealed that bortezomib could inhibit the proliferation of myeloma cells. The apoptotic rates of RPMI-8226 cells in the groups treated with 20, 50 and 80 nmol/l of bortezomib were 12.08±0.61, 35.97±3.11 and 57.22±5.47%, respectively, which were significantly higher than that in the control group (8.28±0.39%) (P<0.05). The expression levels of β-catenin and c-Myc in the experimental groups were significantly lower than those in the control group (P<0.05). Bortezomib can reduce the expression level of Wnt/β-catenin signaling pathway-related proteins, β-catenin and c-Myc, and may inhibit cell proliferation and accelerate apoptosis by activating the Wnt/β-catenin signaling pathway.

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Section: Methodsmentioning

confidence: 99%

Effect of bortezomib on proliferation and apoptosis of myeloma cells by activating Wnt/β‑catenin signaling pathway

2020

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“…PTCs are necessary for gene doping tests and PCR-based detection of viruses, such as COVID-19 [ 6 , 22 ]. However, the use of PTCs comes with the potential risk of sample contamination.…”

Section: Discussionmentioning

confidence: 99%

Design and storage stability of reference materials for microfluidic quantitative PCR-based equine gene doping tests

Tozaki

Ohnuma

Kikuchi

et al. 2021

JES

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One method of gene doping in horseracing is administering of exogenous genetic materials, known as transgenes. Several polymerase chain reaction (PCR)-based methods have been developed for detecting transgenes with high sensitivity and specificity. However, novel designs for reference materials (RMs) and/or positive template controls (PTCs) are necessary for simultaneous analysis of multiple transgene targets. In this study, we designed and developed a novel RM for simultaneously detecting multiple targets via microfluidic quantitative PCR (MFQPCR). Twelve equine genes were selected as targets in this study. A sequence region including primers and probes for quantitative PCR was designed, and a 10 bp sequence was inserted to allow the RM to be distinguished from the original transgene sequences. The sequences of individual detection sites were then connected for 12 genes and cloned into a single plasmid vector. We performed fragment size analysis to distinguish between the PCR products of the original transgene sequence and those of the RM, enabling identification of RM contamination. PTCs diluted to 10,000, 1,000, 100, and 10 copies/µl with horse genomic DNA from RM were stably stored at 4°C for 1 year. As digital PCR enabled absolute quantification, the designed substances can serve as an RM. These findings indicate that the RM design and storage conditions were suitable for gene doping tests using MFQPCR.

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“…Although our prisoners reported a low frequency of homosexual intercourse prevention programs that target these high-risk groups [32]; [33]; [34]; [35].…”

Section: Discussionmentioning

confidence: 99%

High Prevalence Of Alphaherpes Virus 2 (HSV-2) Positivity In Prisons From Midwest Brazil: A Cross-Sectional Study

Almeida

Lima

Puga

et al. 2020

Preprint

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Background: The Herpes simplex virus type 2, currently classified as Human alphaherpesvirus 2 (HSV-2) is the main cause of genital herpes, one of the most prevalent sexually transmitted infections (STIs) in the world. STIs are one of the major public health problems in prisons due to the behaviors of prisoners and the factors that increase the risk of transmission. The present study aimed to estimate the prevalence and risk factors associated with HSV-2 infection at 12 prisons in Mato Grosso of Sul, Brazil. Results: We tested 872 serum samples (732 males and 140 females) for anti-HSV-2 IgG and anti-HSV-2 IgM. The overall HSV-2 IgG prevalence was 43.1%, and the prevalence was greater in males than females (32.1% vs. 11%). The prevalence of HSV-2 in females were 3.4 times more than in males in adjusted model. Among the 184 samples HSV-2 IgG tested for IgM anti HSV-2, 46% were positive. The prevalence of HSV-2 positivity was much greater than reported in the general population of Brazil and in other prison populations worldwide. The multivariable model adjusted, indicated significant and independent associations of HSV-2 IgG positivity with female sex, age older than 29 to 39 years, unmarried status, history of STI, no previous incarceration, being incarcerated with more than 26 others, presence of genital discharge, previous homosexual intercourse, HIV-positivity, and no use/occasional use of condoms during sexual intercourse. Conclusions: This is the first study that evaluated HSV-2 in a prison population in Brazil. These data provide important information needed to implement procedures that better prevent and control of STIs in prison populations.

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Real time PCR to evaluate HSV‐2 shedding from anal and genital samples among men who have sex with men, living with HIV

Cited by 9 publications

References 37 publications

Effect of bortezomib on proliferation and apoptosis of myeloma cells by activating Wnt/β‑catenin signaling pathway

Effect of bortezomib on proliferation and apoptosis of myeloma cells by activating Wnt/β‑catenin signaling pathway

Design and storage stability of reference materials for microfluidic quantitative PCR-based equine gene doping tests

High Prevalence Of Alphaherpes Virus 2 (HSV-2) Positivity In Prisons From Midwest Brazil: A Cross-Sectional Study

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