Performance of human immunostimulating agents in the improvement of fish cytogenetic preparations

Molina, Wagner Franco; Alves, Diego; Araújo, Washington Candeia de; Martínez, P.; Silva, M. F. M.; Costa, Gideão Wagner Werneck Félix da

doi:10.4238/vol9-3gmr840

Cited by 60 publications

(34 citation statements)

References 20 publications

(20 reference statements)

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“…Before chromosome preparation, specimens were subjected to in vivo mitotic stimulation for 24 h by intramuscular and intraperitoneal inoculation of complexes of bacterial and fungal antigens according to the method proposed by Molina et al (2010). The specimens were anesthetized using clove oil (eugenol) and sacrificed for kidney tissue removal.…”

Section: Methodsmentioning

confidence: 99%

Physical mapping of 18S and 5S genes in pelagic species of the genera Caranx and Carangoides (Carangidae)

Jacobina¹,

Bertollo²,

Cioffi³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. In Carangidae, Caranx is taxonomically controversial because of slight morphological differences among species, as well as because of its relationship with the genus Carangoides. Cytogenetic data has contributed to taxonomic and phylogenetic classification for some groups of fish. In this study, we examined the chromosomes of Caranx latus, Caranx lugubris, and Carangoides bartholomaei using classical methods, including conventional staining, C-banding, silver staining for nuclear organizer regions, base-specific fluorochrome, and 18S and 5S ribosomal sequence mapping using in situ hybridization. These 3 species showed chromosome numbers of 2n = 48, simple nuclear organizer regions (pair 1), and mainly centromeric heterochomatin. However, C. latus (NF = 50) and C. bartholomaei (NF = 50) showed a structurally conserved karyotype compared with C. lugubris (NF = 54), with a larger number of 2-armed chromosomes. The richness of GC-positive heterochromatic segments and sites in 5S rDNA in specific locations compared to the other 2 species reinforce 9629 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (4): 9628-9635 (2014) rDNA genes in the chromosomes of Caranx and Carangoides spp the higher evolutionary dynamism in C. lugubris. Cytogenetic aspects shared between C. latus and C. bartholomaei confirm the remarkable phylogenetic proximity between these genera.

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Section: Methodsmentioning

confidence: 99%

Physical mapping of 18S and 5S genes in pelagic species of the genera Caranx and Carangoides (Carangidae)

Jacobina¹,

Bertollo²,

Cioffi³

et al. 2014

Genet. Mol. Res.

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“…The specimens had previously undergone in vivo mitotic stimulation, using attenuated compound antigens, for a period of 24 h [Molina, 2001;Molina et al, 2010]. After this period, the animals were anaesthetized with clove oil and sacrificed for the removal of kidney tissue.…”

Section: Specimens Mitotic Chromosome Procedures and Chromosome Bandingmentioning

confidence: 99%

Transposable Elements in Fish Chromosomes: A Study in the Marine Cobia Species

Costa

Cioffi²,

Bertollo³

et al. 2013

Cytogenet Genome Res

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Rachycentron canadum, a unique representative of the Rachycentridae family, has been the subject of considerable biotechnological interest due to its potential use in marine fish farming. This species has undergone extensive research concerning the location of genes and multigene families on its chromosomes. Although most of the genome of some organisms is composed of repeated DNA sequences, aspects of the origin and dispersion of these elements are still largely unknown. The physical mapping of repetitive sequences on the chromosomes of R. canadum proved to be relevant for evolutionary and applied purposes. Therefore, here, we present the mapping by fluorescence in situ hybridization of the transposable element (TE) Tol2, the non-LTR retrotransposons Rex1 and Rex3, together with the 18S and 5S rRNA genes in the chromosome of this species. The Tol2 TE, belonging to the family of hAT transposons, is homogeneously distributed in the euchromatic regions of the chromosomes but with huge colocalization with the 18S rDNA sites. The hybridization signals for Rex1 and Rex3 revealed a semi-arbitrary distribution pattern, presenting differentiated dispersion in euchromatic and heterochromatic regions. Rex1 elements are associated preferentially in heterochromatic regions, while Rex3 shows a scarce distribution in the euchromatic regions of the chromosomes. The colocalization of TEs with 18S and 5S rDNA revealed complex chromosomal regions of repetitive sequences. In addition, the nonpreferential distribution of Rex1 and Rex3 in all heterochromatic regions, as well as the preferential distribution of the Tol2 transposon associated with 18S rDNA sequences, reveals a distinct pattern of organization of TEs in the genome of this species. A heterogeneous chromosomal colonization of TEs may confer different evolutionary rates to the heterochromatic regions of this species.

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“…The specimens were subjected to mitotic stimulation using attenuated antigen compounds, for a period of 24 to 28 h, according to Molina (2001) and Molina et al (2010). The animals were then anesthetized with clove oil (eugenol) and sacriWced for the removal of anterior kidney fragments and sexed by macroscopic and microscopic examination of the gonads.…”

Section: Specimens and Chromosome Preparationsmentioning

confidence: 99%

Chromosomal differentiation and speciation in sister-species of Grammatidae (Perciformes) from the Western Atlantic

et al. 2011

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In the tropical Atlantic, the ichthyofauna between the coast of Brazil and the Caribbean regions, divided by the Amazon barrier, is very similar presenting several geminate species, including Gramma brasiliensis, endemic in Brazil, and its Caribbean counterpart Gramma loreto. Morphological and molecular studies have helped establish evolutionary patterns that sister-species of these two marine habitats are subjected to. However, their chromosomal characteristics are only beginning to be better characterized. Accordingly, a comparative cytogenetic analysis was carried out in G. brasiliensis and G. loreto, seeking evidence of cytotaxonomic markers implicated in the karyotypic diversiWcation of these species and likely associated with speciation events. Heterochromatic regions and their aYnity to Xuorochromes GC-or AT-speciWc were identiWed, as well as the distribution of ribosomal DNA sites in chromosomes, either by silver nitrate impregnation (Ag-NORs) or dual-color FISH mapping with 18S and 5S rDNA probes. While displaying the same diploid number, 2n = 48 chromosomes, considered basal for Perciformes, the two species diVered in karyotype structure, showing karyotypic formulas and species-speciWc heterochromatin pattern. The cytological characters found support the diVerentiating status of these species, possibly achieved under the conditions of allopatry due to the Amazon/Orinoco barrier, showing chromosomal peculiarities in Grammatidae species when compared to other groups of Perciformes.

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Performance of human immunostimulating agents in the improvement of fish cytogenetic preparations

Cited by 60 publications

References 20 publications

Physical mapping of 18S and 5S genes in pelagic species of the genera Caranx and Carangoides (Carangidae)

Physical mapping of 18S and 5S genes in pelagic species of the genera Caranx and Carangoides (Carangidae)

Transposable Elements in Fish Chromosomes: A Study in the Marine Cobia Species

Chromosomal differentiation and speciation in sister-species of Grammatidae (Perciformes) from the Western Atlantic

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