Performance of Enzyme Separation and Purification by Improved Simulated‐Moving‐Bed Chromatography<sup>a</sup>

Huang, Shih Yow; Lee, Wen Shan

doi:10.1111/j.1749-6632.1988.tb25826.x

Cited by 3 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This kind of system is now commercially available, for example from Resindion srl (Mitsubishi Chemical Corporation), and may be extremely useful for many different biocatalytic processes [131][132][133].…”

Section: Continuous Extraction Of the Antibioticmentioning

confidence: 99%

Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives

Volpato¹,

Rodrigues

Fernández‐Lafuente

2010

CMC

112

View full text Add to dashboard Cite

Semi-synthetic β-lactamic antibiotics are the most used anti-bacteria agents, produced in hundreds tons/year scale. It may be assumed that this situation will even increase during the next years, with new β-lactamic antibiotics under development. They are usually produced by the hydrolysis of natural antibiotics (penicillin G or cephalosporin C) and the further amidation of natural or modified antibiotic nuclei with different carboxylic acyl donor chains. Due to the contaminant reagents used in conventional chemical route, as well as the high energetic consumption, biocatalytic approaches have been studied for both steps in the production of these very interesting medicaments during the last decades. Recent successes in some of these methodologies may produce some significant advances in the antibiotics industry. In fact, the hydrolysis of penicillin G to produce 6-APA catalyzed by penicillin G acylase is one of the most successful historical examples of the enzymatic biocatalysis, and much effort has been devoted to find enzymatic routes to hydrolyze cephalosporin C. Initially this could be accomplished in a quite complex system, using a two enzyme system (D-amino acid oxidase plus glutaryl acylase), but very recently an efficient cephalosporin acylase has been designed by genetic tools. Other strategies, including metabolic engineering to produce other antibiotic nuclei, have been also reported. Regarding the amidation step, much effort has been devoted to the improvement of penicillin acylases for these reactions since 1960. New reaction strategies, continuous product extraction or new penicillin acylases with better properties have proven to be the key to have competitive biocatalytic processes. In this review, a critical discussion of these very interesting advances in the application of enzymes for the industrial synthesis of semi-synthetic antibiotics will be presented.

show abstract

Section: Continuous Extraction Of the Antibioticmentioning

confidence: 99%

Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives

Volpato¹,

Rodrigues

Fernández‐Lafuente

2010

CMC

112

View full text Add to dashboard Cite

show abstract

“…with three peristaltic pumps. The recycle pump (designated as "a" in FIGURE 1) controlled the flow rate of recirculation. In this system, the recycle pump played an important role in the operation of the SMBC.…”

mentioning

confidence: 99%

Performance of Enzyme Separation and Purification by Improved Simulated‐Moving‐Bed Chromatography^a

Huang

Lee

1988

Annals of the New York Academy of Sciences

Self Cite

View full text Add to dashboard Cite

Purification of monoclonal antibodies by simulated moving-bed chromatography

Gottschlich¹,

Kasche²

1997

Journal of Chromatography A

View full text Add to dashboard Cite

Performance of Enzyme Separation and Purification by Improved Simulated‐Moving‐Bed Chromatography^a

Cited by 3 publications

References 3 publications

Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives

Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives

Performance of Enzyme Separation and Purification by Improved Simulated‐Moving‐Bed Chromatography^a

Purification of monoclonal antibodies by simulated moving-bed chromatography

Contact Info

Product

Resources

About

Performance of Enzyme Separation and Purification by Improved Simulated‐Moving‐Bed Chromatographya

Cited by 3 publications

References 3 publications

Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives

Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives

Performance of Enzyme Separation and Purification by Improved Simulated‐Moving‐Bed Chromatographya

Purification of monoclonal antibodies by simulated moving-bed chromatography

Contact Info

Product

Resources

About

Performance of Enzyme Separation and Purification by Improved Simulated‐Moving‐Bed Chromatography^a

Performance of Enzyme Separation and Purification by Improved Simulated‐Moving‐Bed Chromatography^a