Purification of monoclonal antibodies by simulated moving-bed chromatography

Gottschlich, N.; Kasche, Volker

doi:10.1016/s0021-9673(96)00932-6

Cited by 88 publications

(26 citation statements)

References 6 publications

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“…Most of them relate to separation problems that are pseudo-ternary separations, that is, where at least one of the compounds is baselineseparated from the others (Gottschlich and Kasche, 1997;Paredes et al, 2005). The advantages of SMB over batch chromatography are represented by a better productivity and stationary phase utilization.…”

Section: Introduction Mcsgp For Biomolecule Separationmentioning

confidence: 99%

Chromatographic separation of three monoclonal antibody variants using multicolumn countercurrent solvent gradient purification (MCSGP)

Müller‐Späth

Aumann

Melter

et al. 2008

Biotech & Bioengineering

118

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Multicolumn countercurrent solvent gradient purification (MCSGP) is a continuous chromatographic process developed in recent years (Aumann and Morbidelli, 2007a; Aumann et al., 2007) that is particularly suited for applications in the field of bioseparations. Like batch chromatography, MCSGP is suitable for three-fraction chromatographic separations and able to perform solvent gradients but it is superior in terms of solvent consumption, yield, purity, and productivity due to the countercurrent movement of the liquid and the solid phases. In this work, the MCSGP process is applied to the separation of three monoclonal antibody variants on a conventional preparative cation exchange resin. The experimental process performance was compared to simulations based on a lumped kinetic model. Yield and purity values of the target variant of 93%, respectively were obtained experimentally. The batch reference process was clearly outperformed by the MCSGP process.

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Section: Introduction Mcsgp For Biomolecule Separationmentioning

confidence: 99%

Chromatographic separation of three monoclonal antibody variants using multicolumn countercurrent solvent gradient purification (MCSGP)

Müller‐Späth

Aumann

Melter

et al. 2008

Biotech & Bioengineering

118

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“…Ž Thompson, 1997 , andproteins Houwing et al, 1999; . Gottschlich and Kasche, 1997 . So far, size-exclusion chromatography in SMB has only been reported for the fractiona-Ž .…”

Section: Introductionmentioning

confidence: 99%

Mass‐transfer effects during separation of proteins in SMB by size exclusion

2003

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“…6). KASCHE and GOITSCHLICH developed a system which was able to isolate chymotrypsin with 95% activity and > 90 % yield [19] and transferred this to the isolation of monoclonal antibodies [20] by using a protein A-affinity sorbent as the stationary phase.…”

Section: Isolation Of Monoclonal Antibodies By Continuous Affinity Chmentioning

confidence: 99%

Continuous preparative liquid chromatography in the downstrem processing of biotechnological products

Schulte

Britsch

Strube

2000

Acta Biotechnologica

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Continuous counter-current chromatographic processes have been successfully used in the petrochemical and sugar industry over the last 30 years. Only recently has simulated moving bed (SMB)-technology attracted widespread interest in the pharmaceutical industry, mainly as a very efficient system for chromatographic enantioseparation. The application of this technique to the downstream processing of biotechnological products requires some specific changes to meet the special demands of bioproduct isolation. Production processes are set up on an multi-ton scale, for example, for the purification of fructose with both yield and purity higher than 90%. Examples for other mono-and oligosaccharides are reported. In the purification of fatty acids or fat soluble vitamins, SMB technology under supercritical fluid conditions gives additional benefits and increases the productivity by a factor of four when a pressure gradient is applied. Another field of operation is the isolation of drug compounds from natural sources where different batch-and SMB-chromatographic steps could be successfully combined. First examples are reported for cyclosporine A and paclitaxel isolation. Finally, step-gradient elution modes can be used continuously, as demonstrated for the isolation of monoclonal antibodies.

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Purification of monoclonal antibodies by simulated moving-bed chromatography

Cited by 88 publications

References 6 publications

Chromatographic separation of three monoclonal antibody variants using multicolumn countercurrent solvent gradient purification (MCSGP)

Chromatographic separation of three monoclonal antibody variants using multicolumn countercurrent solvent gradient purification (MCSGP)

Mass‐transfer effects during separation of proteins in SMB by size exclusion

Continuous preparative liquid chromatography in the downstrem processing of biotechnological products

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