Performance characteristics of a commercial antibody-capture enzyme immunoassay for detection of Toxoplasma-specific IgM antibodies

Gretch, David R.; Warren, John J.; Bacina, R M; Stefansson, Eric D.; Fritsche, Thomas R.

doi:10.1016/0732-8893(90)90035-t

Cited by 4 publications

(2 citation statements)

References 19 publications

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“…[12][13][14][15][16][17] It should be noted that the Toxo IgG assay showed much higher CVs for the negative pool than for the RV IgG assay having a comparable concentration (5.1 vs 3.1 U/mL).…”

Section: Discussionmentioning

confidence: 99%

Performance Characteristics of Six IMMULITE 2000 TORCH Assays

et al. 2006

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TORCH is an acronym for Toxoplasma gondii (Toxo), other microorganisms (eg, syphilis), rubella virus (RV), cytomegalovirus (CMV), and herpes simplex virus (HSV) that are associated with congenital abnormalities from maternal infection. We evaluated linearity, imprecision, and comparison with commercially available methods of the IMMULITE 2000 (Diagnostic Products, Los Angeles, CA) Toxo IgG, Toxo IgM, RV IgG, RV IgM, CMV IgG, and HSV IgG assays. Linearity and imprecision results were acceptable. The IMMULITE 2000 assays show good concordance with other commercially available methods except for Toxo IgM and RV IgM. Toxo IgM showed better concordance with a consensus of 3 of 4 (Access, Beckman Coulter, Fullerton, CA; IMMULITE 2000; Platelia, Bio-Rad Laboratories Diagnostics Group, Redmond, WA; and Vidas, bioMerieux, Hazelwood, MO) assays than with Access alone. The RV IgM assay showed better concordance with the Zeus method than with the Diamedix method (Diamedix, Miami, FL). The IMMULITE 2000 TORCH assays studied show acceptable performance and are suitable for routine clinical use. Some commercial assays for Toxo IgM and RV IgM show rather poor concordance.

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Section: Discussionmentioning

confidence: 99%

Performance Characteristics of Six IMMULITE 2000 TORCH Assays

et al. 2006

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“…Detection and measurement of IgG-specific antibodies is rarely problematic, and good sensitivity and specificity have been achieved by a variety of methods (2,5,6,8,11). Detection of IgM antibodies is more problematic because of the reported low degree of test specificity and the clinical implications of a false-positive result, which can lead to unnecessary therapeutic intervention (1,4,7). In our study population, which was heavily weighted to include immunocompromised patients (transplant recipients, patients receiving chemotherapy, and AIDS patients), a number were repeatedly reactive in Toxoplasma IgM antibody studies in the absence of IgG seroconversion or other evidence of active toxoplasmosis.…”

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confidence: 99%

Detection of immunoglobulin G (IgG) and IgM antibodies to Toxoplasma gondii: evaluation of four commercial immunoassay systems

et al. 1997

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A comparative evaluation of the following commercial immunoassays for the determination of antibodies to Toxoplasma gondii was performed: Behring Diagnostics OPUS Toxo G and Toxo M, Abbott Diagnostics IMX Toxo-IgG 2.0 and Toxo-IgM, Sanofi Diagnostics Pasteur Platelia Toxo IgG and Toxo IgM, and bioMérieux Vitek VIDAS Toxo IgG and IgM. Of 676 specimens that were tested for Toxoplasma-specific immunoglobulin G (IgG) antibodies, 26% were reactive by all methods while 8% displayed some discrepancy. Of 718 specimens that were tested for Toxoplasma-specific IgM antibodies, 3% were reactive by all methods while 10% displayed some discrepancy. Analysis of discrepant specimens revealed performance shortcomings with all IgM-specific assays. The impact of such shortcomings is magnified in a population with a low prevalence of toxoplasmosis.

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Multicenter evaluation of a new commercial assay for detection of immunoglobulin M antibodies toToxoplasma gondii

Luyasu

Robert

Schaefer

et al. 1995

Eur. J. Clin. Microbiol. Infect. Dis.

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A new commercial assay for detection of IgM-specific antibodies to Toxoplasma gondii (IMx Toxo IgM, Abbott, USA), based on microparticle enzyme immunoassay technology, was evaluated at 15 clinical sites in Europe and the USA. Performance characteristics were established by testing clinical specimens collected randomly from pregnant women, blood donors, individuals with suspected Toxoplasma gondii infection and individuals confirmed HIV positive. Reference testing was performed using Toxo-M EIA (Abbott). Specimens evaluated at European sites yielding discordant results between the new assay and the reference EIA were further tested with an immunosorbent agglutination assay; at sites in the USA, discordant results were resolved using Platelia Toxo IgM (Sanofi, France) and Vidas Toxo IgM (bioMérieux, France) assays. In addition, matched plasma and serum, heat-treated and non-heat-treated specimens, and fresh and frozen specimens were evaluated at the USA sites. At European sites the new commercial assay had a sensitivity of 95.6% (196/205), a specificity of 99.8% (3,137/3,143) and an agreement of 99.6% (3,333/3,348) following resolution of discordant results; sensitivity in the USA was 97.4% (184/189), specificity was 99.8% (1,204/1,207) and agreement was 99.4% (1,388/1,396) following resolution. The new IMx Toxo IgM is a sensitive and specific assay for measurement of IgM antibodies to Toxoplasma gondii in human serum and plasma.

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Performance characteristics of a commercial antibody-capture enzyme immunoassay for detection of Toxoplasma-specific IgM antibodies

Cited by 4 publications

References 19 publications

Performance Characteristics of Six IMMULITE 2000 TORCH Assays

Performance Characteristics of Six IMMULITE 2000 TORCH Assays

Detection of immunoglobulin G (IgG) and IgM antibodies to Toxoplasma gondii: evaluation of four commercial immunoassay systems

Multicenter evaluation of a new commercial assay for detection of immunoglobulin M antibodies toToxoplasma gondii

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