Performance Characteristics of Six IMMULITE 2000 TORCH Assays

Abstract

TORCH is an acronym for Toxoplasma gondii (Toxo), other microorganisms (eg, syphilis), rubella virus (RV), cytomegalovirus (CMV), and herpes simplex virus (HSV) that are associated with congenital abnormalities from maternal infection. We evaluated linearity, imprecision, and comparison with commercially available methods of the IMMULITE 2000 (Diagnostic Products, Los Angeles, CA) Toxo IgG, Toxo IgM, RV IgG, RV IgM, CMV IgG, and HSV IgG assays. Linearity and imprecision results were acceptable. The IMMULITE 2000 assays show good concordance with other commercially available methods except for Toxo IgM and RV IgM. Toxo IgM showed better concordance with a consensus of 3 of 4 (Access, Beckman Coulter, Fullerton, CA; IMMULITE 2000; Platelia, Bio-Rad Laboratories Diagnostics Group, Redmond, WA; and Vidas, bioMerieux, Hazelwood, MO) assays than with Access alone. The RV IgM assay showed better concordance with the Zeus method than with the Diamedix method (Diamedix, Miami, FL). The IMMULITE 2000 TORCH assays studied show acceptable performance and are suitable for routine clinical use. Some commercial assays for Toxo IgM and RV IgM show rather poor concordance.

Evaluation of DPC immulite 2000®Toxoplasma quantitative IgG/IgM kits for automated toxoplasmosis serology with immulite 2000®

“…However, we can draw from the results obtained with the PI with persistent IgM cohort that kinetics of IgM detection and sensitivity using Vidas s and Immulite 2000 s are similar (around 70%), though imperfectly correlated (concordance test of 86%). These results are in agreement with the study of Owen et al (13). By contrast, results obtained with Access II showed a higher detection of residual IgM (96%), most results being highly correlated to Immulite 2000 s (concordance test of 93%).…”

Bicentric Evaluation of Six Anti-Toxoplasma Immunoglobulin G (IgG) Automated Immunoassays and Comparison to the Toxo II IgG Western Blot

“…In Europe, the Access II test replaced the Access I test during the year 2008. The Access I (4,8), AxSYM (4,8,11,19), and Vidas (6,8,11,17) tests use the WHO second international standard (IS), whereas the Access II, Immulite (10), and Modular (6) tests use the third WHO IS. It is to be noted that neither the second nor the third WHO IS was tested in parallel by the six automated techniques in the present study.…”

Evaluation of Eight Anti-Rubella Virus Immunoglobulin G Immunoassays That Report Results in International Units per Milliliter

“…Immulite 2000 Rubella Quantitative IgG (Diagnostic Products Corporation, CA) (Immulite) (8,20,30) used beads coated with inactivated rubella virus as the solid phase and alkaline phosphatase conjugated to monoclonal murine antihuman IgG as the conjugate. Chemiluminescent substrate was used to detect the antibody-antigen reaction.…”