Peptidase inhibitors from the salivary glands of the cockroach Nauphoeta cinerea

Taranushenko, Yuliya; Vinokurov, Konstantin S.; Kłudkiewicz, Barbara; Kodrı́k, Dalibor; Sehnal, František

doi:10.1016/j.ibmb.2009.11.002

Cited by 3 publications

(3 citation statements)

References 52 publications

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“…They used boiled extracts to inactivate endogenous enzymes, as in the current study, and they found up to 80% protease inhibition in the posterior half of the midgut. Recently, endogenous protease inhibitors have been described in the digestive system of cockroaches (Taranushenko et al, 1909; Elpidina et al, 2001) using whole gut extracts (epithelium plus lumen contents). Inhibition of trypsin and aminopeptidase occurs in the tissue extracts and in the lumen of S. frugiperda (current study), which is the first report of endogenous digestive enzyme inhibitors in a lepidopteran species.…”

Section: Discussionmentioning

confidence: 99%

“…However, divalent ions, especially Ca, seem to confer a general resistance of α‐amylase to proteolytic degradation in vertebrates (Stein and Fischer, 1958), and this probably holds true in S. frugiperda . Other authors suggest that endogenous protease inhibitors might be important as protection against fungi and bacteria that employ subtilisins to attack insect tissues (Taranushenko et al, 2009; Vinokurov et al, 2007). This seems to be especially appropriate in cockroaches, where the salivary inhibitors could protect the insect against the massive amounts of bacteria that are stored over long periods in the crop (Taranushenko et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Protease inhibitors of the Kazal type are found in the salivary gland and saliva of Nauphoeta cinerea , which are particularly effective against the bacterial protease subtilisin. This suggests a defensive mechanism against the masses of bacteria typically found in the cockroach crop, some of which are probably pathogenic (Taranushenko et al, 1909). Inhibitory peptides in the midgut lumen are less well studied, though trypsin and subtilisin inhibitors are described from whole midgut preparations of the cockroach N. cinerea (Elpidina et al, 2001), and in five additional cockroach species (Vinokurov et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Exogenous and endogenous protease inhibitors in the gut of the fall armyworm larvae, Spodoptera frugiperda

Lwalaba

Weidlich

Hoffmann

et al. 2010

Arch Insect Biochem Physiol

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A dose-dependent inhibition of endogenous trypsin and aminopeptidase occurs in the lumen of Spodoptera frugiperda after feeding L6 larvae exogenous inhibitors soybean trypsin inhibitor (SBTI), tosyl-L-lysine chloromethyl ketone-HCl (TLCK), or bestatin, respectively, for 3 days. TLCK inhibits trypsin in tissue extracts and in secretions more strongly than SBTI. The aminopeptidase released into the lumen (containing the peritrophic membrane) is strongly inhibited by bestatin, but the membrane-bound enzyme is not. A bound enzyme may be more resistant to an inhibitor than unbound. A cross-class elevation of aminopeptidase activity occurs in response to ingested trypsin inhibitor, but there was no cross-class effect of aminopeptidase inhibitor (bestatin) on trypsin activity. An endogenous trypsin and aminopeptidase inhibitor is present in the lumen and ventricular cells. The strength of the endogenous trypsin inhibition seems to be in the same range as that resulting from ingestion of the exogenous inhibitor SBTI. In some insect species, considerable trypsin secretion occurs in unfed as well as in fed animals, and endogenous protease inhibitors might function to protect the ventricular epithelium by inactivation of trypsin when less food is available.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exogenous and endogenous protease inhibitors in the gut of the fall armyworm larvae, Spodoptera frugiperda

Lwalaba

Weidlich

Hoffmann

et al. 2010

Arch Insect Biochem Physiol

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Role of endogenous inhibitors on digestive amylolytic and proteolytic activities of a predatory hemipteran,Andrallus spinidensFabricius

Sorkhabi-Abdolmaleki

Zibaee

2013

Archives Of Phytopathology And Plant Protection

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Digestive proteolytic activity in Apodiphus amygdali Germar (Hemiptera: Pentatomidae): effect of endogenous inhibitors

Ramzi

Zibaee

2014

J Entomol Acarol Res

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The digestive proteolytic profile of Apodiphus amygdali was determined by using several substrates and specific inhibitors. Analysis of optimal pH and temperature showed the highest enzymatic activity at the pH range of 6-7 and temperature of 40°C when azocasein was used as a substrate. By using a negative control, the presence of several specific proteases were determined including tryspin-like, chymotrypsinlike, elastase, cathepsin B, cathepsin L, amino- and carboxypeptidases in the midgut content of A. amygdali, with the highest and the lowest activities of cathepsin L and carboxypeptidase, respectively. pH dependency of specific proteases revealed optimal pHs of 9, 8 and 9 for trypsin-, chymotrypsin-like, 6 for cathepsins and 5-6 for carboxy- and aminopeptidases, respectively. Specific inhibitors, including phenylmethylsulfonyl fluoride, Na-p-tosyl-L-lysine chloromethyl ketone, Ntosyl- L-phenylalanine chloromethyl ketone, L-trans-epoxysuccinylleucylamido-(4-guanidino)-butane, phenanthroline and ethylendiamidetetraacetic acid, significantly decreased proteolytic activity, indicating the presence of different proteases in the midgut of A. amygdali. Extracted inhibitors from the midgut demonstrated significant inhibition of specific proteolytic activities of A. amygdali except for cathepsin B and aminopeptidase. The results indicated that determination of digestive proteolytic activity could be helpful to clarify digestion process in insects. Moreover, understanding the nature of digestive proteases might be used to develop several inhibitors for providing resistant crop varieties against pests.

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Peptidase inhibitors from the salivary glands of the cockroach Nauphoeta cinerea

Cited by 3 publications

References 52 publications

Exogenous and endogenous protease inhibitors in the gut of the fall armyworm larvae, Spodoptera frugiperda

Exogenous and endogenous protease inhibitors in the gut of the fall armyworm larvae, Spodoptera frugiperda

Role of endogenous inhibitors on digestive amylolytic and proteolytic activities of a predatory hemipteran,Andrallus spinidensFabricius

Digestive proteolytic activity in Apodiphus amygdali Germar (Hemiptera: Pentatomidae): effect of endogenous inhibitors

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