Role of endogenous inhibitors on digestive amylolytic and proteolytic activities of a predatory hemipteran,Andrallus spinidensFabricius

Sorkhabi-Abdolmaleki, Sahar; Zibaee, Arash

doi:10.1080/03235408.2013.807558

Cited by 2 publications

(5 citation statements)

References 12 publications

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“…Bigham & Hosseininaveh (2010) observed the presence of general protease activity by acidic and alkaline optima in the midgut extract of B. germari using hemoglobin as a substrate. Sorkhabi-Abdolmaleki et al (2013) found an alkaline pH for general proteolytic activity, serine proteases and two exopeptidases with another peak at pH 6, as with the cysteine proteases. The optimal pH of specific proteases corresponds with the expected values, as noted in the literature (Terra & Ferreira, 2012).…”

Section: Discussionmentioning

confidence: 77%

“…Endogenous inhibitors may have several functions, such as protection of a-amylase from proteolytic activity (Stein & Fischer, 1958), attacking fungi and bacteria in gut (Taranushenko et al, 2009) and protection of epithelial cells from digestive enzymes when food is not present in the midgut (Lwalaba et al, 2010) cantly decreased trypsin and chymotrypsin activities. All extracted inhibitors significantly decreased elastase and cathepsin activities of A. spinidens except for C. suppressslis (Sorkhabi-Abdolmaleki & Zibaee, 2013). Extracted inhibitors from all species significantly decreased cathepsins B and L activities, but this decrease was not significantly different in the case of C. suppressalis.…”

Section: Discussionmentioning

confidence: 83%

“…Bigham & Hosseininaveh (2010) found negative effects of E-64 on the proteolytic activity of A. spinidens. Sorkhabi-Abdolmaleki et al (2013) observed that E-64 and cystatin decreased proteolytic activity in the midgut of A. spinidens.…”

Section: Discussionmentioning

confidence: 84%

“…Extracted inhibitors from all species significantly decreased cathepsins B and L activities, but this decrease was not significantly different in the case of C. suppressalis. In the case of exopeptidases, endogenous inhibitors from E. kuehniella and H. cunea on aminopeptidase and all inhibitors on carboxypeptidase significantly decreased enzymatic activities (Sorkhabi-Abdolmaleki & Zibaee, 2013).…”

Section: Discussionmentioning

confidence: 91%

“…Zhu et al (2003) found serine proteases as the major enzymes in the gut of L. lineolaris by TLCK (Zhu et al, 2003). Bigham & Hosseininaveh (2010) and Sorkhabi et al (2013) found significant inhibition of digestive proteases by PMSF, TLCK and TPCK in B. germary and A. spinidens. Presence of cysteine proteases was confirmed by using specific substrates, cystatin, E-64 and DTT, although the experiment with a negative control showed cathepsin L as the major protease in the midgut of A.…”

Section: Discussionmentioning

confidence: 96%

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Digestive proteolytic activity in Apodiphus amygdali Germar (Hemiptera: Pentatomidae): effect of endogenous inhibitors

Ramzi

Zibaee

2014

J Entomol Acarol Res

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The digestive proteolytic profile of Apodiphus amygdali was determined by using several substrates and specific inhibitors. Analysis of optimal pH and temperature showed the highest enzymatic activity at the pH range of 6-7 and temperature of 40°C when azocasein was used as a substrate. By using a negative control, the presence of several specific proteases were determined including tryspin-like, chymotrypsinlike, elastase, cathepsin B, cathepsin L, amino- and carboxypeptidases in the midgut content of A. amygdali, with the highest and the lowest activities of cathepsin L and carboxypeptidase, respectively. pH dependency of specific proteases revealed optimal pHs of 9, 8 and 9 for trypsin-, chymotrypsin-like, 6 for cathepsins and 5-6 for carboxy- and aminopeptidases, respectively. Specific inhibitors, including phenylmethylsulfonyl fluoride, Na-p-tosyl-L-lysine chloromethyl ketone, Ntosyl- L-phenylalanine chloromethyl ketone, L-trans-epoxysuccinylleucylamido-(4-guanidino)-butane, phenanthroline and ethylendiamidetetraacetic acid, significantly decreased proteolytic activity, indicating the presence of different proteases in the midgut of A. amygdali. Extracted inhibitors from the midgut demonstrated significant inhibition of specific proteolytic activities of A. amygdali except for cathepsin B and aminopeptidase. The results indicated that determination of digestive proteolytic activity could be helpful to clarify digestion process in insects. Moreover, understanding the nature of digestive proteases might be used to develop several inhibitors for providing resistant crop varieties against pests.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 91%