Penicillin G and penicillin G-tinidazole treatment of experimentally induced summer mastitis — effect on elimination rates of bacteria and outcome of the disease

Hirvonen, J.; Pyörälä, Satu; Heinäsuo, Aila; Jousimies‐Somer, Hannele

doi:10.1016/0378-1135(94)90062-0

Cited by 10 publications

(10 citation statements)

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“…The acute-phase response of bovine ITIH4 has been studied in our laboratory in two experimental infection models, with animals with induced summer mastitis (23,24) and with animals inoculated with the common bovine respiratory pathogen BRSV. The experimental mastitis model used in this work resulted in clinical mastitis in all 10 heifers that were treated.…”

Section: Discussionmentioning

confidence: 99%

“…Ten pregnant heifers (eight Ayrshires and two Friesians) due to calve in 1 or 2 months were experimentally infected as previously described (23). Briefly, two hindquarters of each heifer were inoculated with a suspension prepared by combining 1 ml of each of the following bacterial strains (9 ϫ 10 8 CFU/ml) recently isolated from field cases of summer mastitis (24): Actinomyces pyogenes AHP 13199, Fusobacterium necrophorum AHN 8155, and Peptostreptococcus indolicus AHC 5732.…”

Section: Methodsmentioning

confidence: 99%

“…Several clinical parameters, including body temperature and udder swelling, were also monitored (23). Antibiotic treatment of the heifers started 32 h after bacterial challenge and lasted for 3 days (23,24). The acute-phase response of the heifers was investigated using conventional APP, and the results were published earlier (23).…”

Section: Methodsmentioning

confidence: 99%

“…The acute-phase response of the protein was studied in experimental infection models in adult and young cattle: cows with induced summer mastitis (23,24) and calves inoculated with the common pathogen bovine respiratory syncytial virus (BRSV) (41).…”

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confidence: 99%

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ITIH4 (Inter-Alpha-Trypsin Inhibitor Heavy Chain 4) Is a New Acute-Phase Protein Isolated from Cattle during Experimental Infection

et al. 2004

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We have isolated from calf serum a protein with an apparent M r of 120,000. The protein was detected by using antibodies against major acute-phase protein in pigs with acute inflammation. The amino acid sequence of an internal fragment revealed that this protein is the bovine counterpart of ITIH4, the heavy chain 4 of the inter-alpha-trypsin inhibitor family. The response of this protein in the sera was determined for animals during experimental bacterial and viral infections. In the bacterial model, animals were inoculated with a mixture of Actinomyces pyogenes, Fusobacterium necrophorum, and Peptostreptococcus indolicus to induce an acute-phase reaction. All animals developed moderate to severe clinical mastitis and exhibited remarkable increases in ITIH4 concentration in serum (from 3 to 12 times the initial values, peaking at 48 to 72 h after infection) that correlated with the severity of the disease. Animals with experimental infections with bovine respiratory syncytial virus (BRSV) also showed increases in ITIH4 concentration (from two-to fivefold), which peaked at around 7 to 8 days after inoculation. Generally, no response was seen after a second infection of the same animals with the virus. Because of the significant induction of the protein in the animals in the mastitis and BRSV infection models, we can conclude that ITIH4 is a new positive acute-phase protein in cattle.The acute-phase response occurs in animals as a consequence of infection, inflammation, or trauma. It is a nonspecific response mediated by inflammation-related cytokines (mainly by interleukin-6 [IL-6], IL-1, and tumor necrosis factor alpha) and is characterized by several systemic reactions, including fever, catabolism of muscle protein, alterations in sleep and appetite patterns, and changes in the concentrations of a group of serum proteins called acute-phase proteins (APP) (3,22,27,40).APP have been studied extensively in humans and in many animal species, mostly rats (38), though the panel of these proteins is probably not complete. The APP response differs among species. For domestic animals, the studies on APP are relatively recent (reviewed in reference 18). For cattle, several APP, including serum amyloid A, haptoglobin, alpha-1-acid glycoprotein, and alpha-1-proteinase inhibitor (alpha-1-antitrypsin), have been shown to increase in different inflammatory processes, in both in vivo (9,14,18,20,23,25,32) and in vitro models (2, 31). The course of infection in human patients is monitored by the determination of APP in blood samples (43), and a similar clinical use has been proposed for veterinary medicine (15,18,26) as well as for monitoring the health status of animals during farm production and at slaughter (42).In previous works from our laboratory, a new 120-kDa plasma glycoprotein, designated major acute-phase protein or pig MAP, was described as an APP occurring in pigs with turpentine-induced inflammation (16,29). This protein, which is also called porcine inter-alpha-trypsin inhibitor human-related protein (pig IHRP) (...

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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ITIH4 (Inter-Alpha-Trypsin Inhibitor Heavy Chain 4) Is a New Acute-Phase Protein Isolated from Cattle during Experimental Infection

et al. 2004

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“…Bacterial biofilms, embedded in a self-produced polymer matrix consisting of polysaccharide, protein, and DNA, show an increased tolerance to antibiotics and disinfectants as well as resistance to phagocytosis and other components of the immune system; therefore, they result in chronic infections (Høiby et al, 2010). Previous studies showed that the intramammary administration of antibiotics against T. pyogenes infections was ineffective (Jousimies-Somer et al, 1996), whereas prolonged and intensive systemic treatment of early cases with beta-lactams and rifampin was occasionally effective (Hirvonen et al, 1994;Andrews et al, 2004;Zhang et al, 2014).…”

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Properties and antimicrobial susceptibility of Trueperella pyogenes isolated from bovine mastitis in China

Alkasir

Wang

Gao

et al. 2016

Acta Veterinaria Hungarica

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Trueperella (T.)pyogenes is an opportunistic pathogen that causes suppurative diseases in domestic animals. In this work, the properties, pathogenesis and phenotypic diversity of T. pyogenes isolates from bovine mastitis were studied. Both pyolysin (plo) and collagen-binding protein (cbp) virulence factor genes were detected by PCR in all T. pyogenes isolates (n = 50). Using the tissue culture plate method, 90% of T. pyogenes isolates were able to form biofilms. The minimum inhibitory concentrations (MICs) of 13 antimicrobials against T. pyogenes isolates were determined. High susceptibility was observed to rifampin (96%), ampicillin (94%), ciprofloxacin (94%), and penicillin (92%), while low susceptibility was found to trimethoprim-sulphamethoxazole (10%) and bacitracin (2%). The intracellular assay revealed that T. pyogenes isolates had different cytopathogenic effects on cells. The high percentage (28.6%) of T. pyogenes isolates suggests that this bacterium is an important contributor to mastitis. Moreover, the high occurrence of multidrug resistance, biofilm production, intracellular survival, and the temporal dynamics of T. pyogenes interactions are key factors for a better understanding of how immunity acts on infections with these bacteria and how they evade immune surveillance, thus highlighting the need for the prudent use of antimicrobial agents in veterinary medicine.Key words: Trueperella pyogenes, bovine mastitis, biofilm, virulence factor, antimicrobial susceptibility Mastitis is an inflammation of the mammary gland caused by several varieties of bacteria such as Staphylococcus (S.) aureus, S. epidermidis, and strep- † Equal contributors

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Proteomic characterization by 2-DE in bovine serum and whey from healthy and mastitis affected farm animals

Alonso-Fauste

Andrés

Iturralde

et al. 2012

Journal of Proteomics

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Penicillin G and penicillin G-tinidazole treatment of experimentally induced summer mastitis — effect on elimination rates of bacteria and outcome of the disease

Cited by 10 publications

References 7 publications

ITIH4 (Inter-Alpha-Trypsin Inhibitor Heavy Chain 4) Is a New Acute-Phase Protein Isolated from Cattle during Experimental Infection

ITIH4 (Inter-Alpha-Trypsin Inhibitor Heavy Chain 4) Is a New Acute-Phase Protein Isolated from Cattle during Experimental Infection

Properties and antimicrobial susceptibility of Trueperella pyogenes isolated from bovine mastitis in China

Proteomic characterization by 2-DE in bovine serum and whey from healthy and mastitis affected farm animals

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