Proteomic characterization by 2-DE in bovine serum and whey from healthy and mastitis affected farm animals

Proteomics of inflammatory and oxidative stress response in cows with subclinical and clinical mastitis

“…The first one was interalpha-trypsin inhibitor heavy chain H4; it is an acute phase protein and its concentration was found to follow an increasing trend starting from the control to the clinical group, but a bigger difference was found between the subclinical and the clinical group. It is a 120 kDa protein and its concentration during clinical mastitis has already being studied [37,77], but it has never been studied during subclinical mastitis. Its increasing trend in concentration between control and clinical mastitis suggests that it can be used as a marker for monitoring the inflammation status.…”

“…Differential expression analysis performed by Baeker et al [37,38] on the whey from both mastitic and non-mastitic milk revealed a marked increase in the expression of four protein spots during infection. Peptide mass fingerprinting (PMF) analysis revealed all four spots as prostaglandin-D synthase (PGDS).…”

“…Most of proteomic studies on mastitis have been performed on milk and somatic cells [35][36][37]. Differential expression analysis performed by Baeker et al [37,38] on the whey from both mastitic and non-mastitic milk revealed a marked increase in the expression of four protein spots during infection.…”

Farm animal milk proteomics

“…The experiments were conducted with a conventional proteomic approach using 2D electrophoresis as a choice of separation method, coupled by MALDI TOF analysis for identification [20]. Another interesting approach that could be used for differential analysis of milk proteome is represented by twodimensional difference gel electrophoresis (2D DIGE).…”

Potent cough suppression by physiologically active substance in human plasma

“…The data were used to search a compiled protein database that was composed of a public protein database (http://www.ncbi.nlm.nih.gov/). 2-DE electrophoresis and analyses of the target protein bands by MALDI/TOF-MS or LC-MS/MS were done at APRO life Science Institute (Tokushima, JP) (Ishida et al 2006;Alonso-Faustea et al 2012). Each equivalent amount (120 μg) of the samples G1, pool-6 and pool-5 was separated on 2-DE.…”