Penicillin enzyme biosensors based on pH membrane electrodes

Koncki, Robert; Leszczyńska, Ewa; Cybulska, Aleksandra; Głąb, Stanisław

doi:10.1016/0003-2670(95)00547-1

Cited by 20 publications

(5 citation statements)

References 30 publications

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“…In another interesting study the performance of aminated PVC and carboxylated PVC‐based pH sensitive ISEs in the analysis of penicillin were compared 48, 84. Enzyme penicillinase, which catalyses the hydrolysis of penicillin to penicillonic acid, was immobilized on the membranes surfaces using a variety of methods.…”

Section: Pvc Modificationmentioning

confidence: 99%

Chemical Modification of Polymer Ion‐Selective Membrane Electrode Surfaces

Pawlak

Bakker

2014

Electroanalysis

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Membrane/sample interactions play a crucial role in the biomedical application of ion selective electrodes (ISEs). Modulation of membrane surface properties can be used as a way to enhance biocompatibility and in fabrication of biosensors through biomolecules immobilization. This review discusses advances in methods and materials used for the surface modifications of polymeric ion-selective membranes in view of their applications in clinical diagnostics

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Section: Pvc Modificationmentioning

confidence: 99%

Chemical Modification of Polymer Ion‐Selective Membrane Electrode Surfaces

Pawlak

Bakker

2014

Electroanalysis

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“…It was made by placing freshly prepared solution of the enzyme on the membrane. This method of enzyme attaching to the surface is very simple and useful for measurements under flow analysis conditions (it was described in details in [34]). The biosensors were preconditioned six hours before measurements, and then calibration curves were recorded.…”

Section: Biosensorsmentioning

confidence: 99%

Potentiometric Studies and Various Applications of Solid State Electrodes Based on Silicon and Epoxy Glass Structures – an Overview

et al. 2009

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Potentiometric microelectrodes are attractive sensor tools for the determination of ionic components in liquids, in wide area of applications, from environmental monitoring to medical diagnostics. Appropriate design of miniaturized transducers based on solid-state technology and the fabrication of dedicated flow multiparameter systems allow the reduction of sample volume and reagents loss. In this overview various examples of the design, performance, and applications of solid-state potentiometric microelectrodes based on back-side contact transducers for flow-cell analysis are presented. The presented results summarize the research works on that field that were conduced in CSRG/Warsaw University of Technology.

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“…Because antibiotics are frequently administered in large doses, it is sometimes important to be able to monitor their concentrations in serum, especially in patients with impaired renal function. Several reports for the determination of penicillin antibiotics have been developed, including chemiluminescence,7, 8 potentiometry and amperometry using biosensors,9–18 potentiometric ion‐selective electrodes based on anion exchangers,19, 20 capillary electrophoresis,21 spectrophotometry,22–25 liquid chromatography,26, 32 high performance liquid chromatography (HPLC),28, 29 direct current and differential pulse polarography (DPP),30 tandem mass spectrometry,27, 32 etc . HPLC was very useful for the determination of trace penicillin antibiotics but it needed complex pretreatments.…”

Section: Introductionmentioning

confidence: 99%

Quantification of sub‐nanomolar levels of Penicillin G by differential pulse adsorptive stripping voltammetry

Abbasi

Khodarahmian

Farmany

2011

Drug Testing and Analysis

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A novel selective and sensitive method is developed for determination of Penicillin G by Differential Pulse Adsorptive Stripping Voltammetry (DPAdSV). Penicillin G gave well-resolved diffusion-controlled cathodic peaks at -0.42 and -0.584 V, respectively (vs Ag/AgCl) in pH 7.50 of borate buffer. Optimal conditions were obtained as pH 7.50, accumulation potential of -0.2 V (vs Ag/AgCl), accumulation time of 120 s, and scan rate of 100 mV/s. Under the optimized conditions, a linear calibration curve was established for the concentration of Penicillin G in the range of 0.007-2.13 µg/ml with a detection limit of 0.000717 µg/ml. The procedure was successfully applied to the determination of Penicillin G in various medicine and biological samples. The relative standard deviation of the method at 0.05 and 0.5 µg/ml Penicillin G, for 10 runs, was 2.55% and 2.06%, respectively.

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Penicillin enzyme biosensors based on pH membrane electrodes

Cited by 20 publications

References 30 publications

Chemical Modification of Polymer Ion‐Selective Membrane Electrode Surfaces

Chemical Modification of Polymer Ion‐Selective Membrane Electrode Surfaces

Potentiometric Studies and Various Applications of Solid State Electrodes Based on Silicon and Epoxy Glass Structures – an Overview

Quantification of sub‐nanomolar levels of Penicillin G by differential pulse adsorptive stripping voltammetry

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