Quantification of sub‐nanomolar levels of Penicillin G by differential pulse adsorptive stripping voltammetry

Abbasi, Shahryar; Khodarahmian, K.; Farmany, Abbas

doi:10.1002/dta.256

Cited by 6 publications

(4 citation statements)

References 31 publications

(26 reference statements)

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“…The experimental pK a s values (at room temperature) used in this study are taken from the literature [17][18][19][20][21][22][23][24][25][26][27][28][29][30]. In reference papers, the experimental information related to each pK a values is given.…”

Section: Experimental Datamentioning

confidence: 99%

Theoretical Determination of the pK a Values of Betalamic Acid Related to the Free Radical Scavenger Capacity: Comparison Between Empirical and Quantum Chemical Methods

Tutone

Lauria

Almerico

2015

Interdiscip Sci Comput Life Sci

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Health benefits of dietary phytochemicals have been suggested in recent years. Among 1000s of different compounds, Betalains, which occur in vegetables of the Cariophyllalae order (cactus pear fruits and red beet), have been considered because of reducing power and potential to affect redox-modulated cellular processes. The antioxidant power of Betalains is strictly due to the dissociation rate of the acid moieties present in all the molecules of this family of phytochemicals. Experimentally, only the pK avalues of betanin were determined. Recently, it was evidenced it was evidenced as the acid dissociation, at different environmental pHs, affects on its electron-donating capacity, and further on its free radical scavenging power. The identical correlation was studied on another Betalains family compound, Betalamic Acid. Experimental evidences showed that the free radical scavenging capacity of this compound drastically decreases at pH [ 5, but pK a values were experimentally not measured. With the aim to justify the Betalamic Acid behavior as free radical scavenger, in this paper we tried to predict in silico the pK a values by means different approaches. Starting from the known experimental pK a s of acid compounds, both phytochemicals and small organic, two empirical approaches and quantum-mechanical calculation were compared to give reliable prediction of the pK a s of Betalamic Acid.Results by means these computational approaches are consistent with the experimental evidences. As shown herein, in silico, the totally dissociated species, at the experimental pH [ 5 in solution, is predominant, exploiting the higher electron-donating capability (HOMO energy). Therefore, the computational estimated pK a values of Betalamic Acid resulted very reliable.

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Section: Experimental Datamentioning

confidence: 99%

Theoretical Determination of the pK a Values of Betalamic Acid Related to the Free Radical Scavenger Capacity: Comparison Between Empirical and Quantum Chemical Methods

Tutone

Lauria

Almerico

2015

Interdiscip Sci Comput Life Sci

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“…A further aspect to be considered is the acidity of GA (pK a COOH ≈ 4.5, pK a OH ≈ 10) [Dwibedy et al, 1999;Abbasi et al, 2011] which is stronger than TA (pK a OH ≈ 6-10 [ Krogsgaard et al, 2016;Fan et al, 2017]. This is because the GA molecule contains free -COOH units whereas in TA, this unit forms linkages -COOR.…”

Section: Discussionmentioning

confidence: 99%

Incorporation of Green Capping Agents to Reduce Silver-Mediated Dentine Staining

et al. 2022

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Silver diammine fluoride (SDF) is known as a non-invasive, cost-effective, safe and simple method of dental caries treatment. However, staining and discoloration seem inseparable with SDF and continue as a cosmetic concern. Research is ongoing to overcome these issues for example by using glutathione or potassium iodide among others. Therefore, the study aimed to investigate the effects of incorporating different concentrations of capping agents on SDF chemistry and SDF-mediated tooth staining at different time points. Tannic acid (TA), gallic acid (GA), carboxymethyl chitosan (CM), and glutathione (G) at different concentrations (5, 10, and 15% w/v) were incorporated in 30% SDF. FTIR and UV-Vis spectroscopy of the prepared solutions was performed to evaluate chemical changes. Time-dependent color changes (ΔE) in bovine dentine specimens (6×6×1 ± 0.25 mm3) were measured spectrophotometrically at: application/washup, 1 and 3hrs, after 1,2,4,7, and 14 days. Results showed suppression of FTIR peaks at 3358cm-1 and 1215cm-1 in capping agent modified SDF indicative of a successful capping effect of the silver ions, which was corroborated by UV-Vis blueshift of ~∆32nm. The capping effect on SDF increased proportionally with the concentration of TA, GA, CM and G used. A more pronounced tooth staining reduction however, shown more in TA- and G- rather than in GA- and CM-modified SDF. At day-14, SDF showed the highest mean ΔE(50.14±2.14), while 15% TA showed the lowest ΔE(30.14±0.81). In conclusion, capping agent incorporation significantly reduced SDF-mediated tooth staining. This reduction in staining is more dependent on the respective capping agent functional groups than concentrations per se. The potential of capping agents to minimize tooth staining of SDF was TA>G>CM>GA.

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“…Therefore, the development of analytical methodologies of antibiotics, and in particular ␤-lactams, has been an active research field [5] that includes developments with chromatographic techniques [6][7][8][9], capillary electrophoresis [10], voltammetry [11], and a few examples using amperometric measurements can also be found in literature [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

β-Lactamase-based biosensor for the electrochemical determination of benzylpenicillin in milk

Prado

Foguel

Gonçalves

et al. 2015

Sensors and Actuators B: Chemical

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Quantification of sub‐nanomolar levels of Penicillin G by differential pulse adsorptive stripping voltammetry

Cited by 6 publications

References 31 publications

Theoretical Determination of the pK a Values of Betalamic Acid Related to the Free Radical Scavenger Capacity: Comparison Between Empirical and Quantum Chemical Methods

Theoretical Determination of the pK a Values of Betalamic Acid Related to the Free Radical Scavenger Capacity: Comparison Between Empirical and Quantum Chemical Methods

Incorporation of Green Capping Agents to Reduce Silver-Mediated Dentine Staining

β-Lactamase-based biosensor for the electrochemical determination of benzylpenicillin in milk

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