Pengembangan Model Hewan Coba Tikus Diabetes Mellitus Tipe 2 dengan Induksi Diet Tinggi Fruktosa Intragastrik

Wulansari, Devyani Diah; Wulandari, Devyana Dyah

doi:10.24123/mpi.v2i1.1302

Cited by 6 publications

(8 citation statements)

References 4 publications

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“…This result proved that fructose ingestion via intragastric tube could increase body weight of mice significantly. This results had the same pattern with the research conducted by Wulansari 12 .…”

Section: Discussionsupporting

confidence: 89%

“…The rate of fructolysis is not inhibited by its products. 12 High fructose in diet can serve as a relatively unregulated source of acetyl-CoA. Therefore, ingestion of high concentration of fructose can increase the rate of de novo lipogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…The dose of fructose solution was 270 grams for 9 days. 12 Mice were terminated after the treatment had finished, and then the hippocampus tissue was incised. The hippoccampus tissues were minced to small pieces and rinsed in ice-cold Phosphate-buffered saline (PBS) to eliminate excess blood entirely.…”

Section: Methodsmentioning

confidence: 99%

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Exercise Intensity May affect Bdnf Level in the Hippocampus of Fructose-Induced Mice

Munir¹,

Miftahussurur²,

Pranoto³

et al. 2021

IJFMT

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Introduction -Several studies show that there is a negative correlation between obesity and cognitive function. Exercise can reverse the bad effect of obesity through mediation of BDNF. Research about the effect of exercise intensity to BNDF levels in hippocampus of mice is ever be done, but research about the effect of exercise intensity to BNDF levels in hippocampus of fructose-induced mice is never be done.Objective -To prove the effect of different exercise intensity to BDNF level in hippocampus of fructoseinduced mice.Methods -A total of 32 mice were divided into four groups: Control group which was a CON and treatment groups which were low-intensity exercise (LIE), moderate-intensity exercise (MIE), and high-intensity exercise (HIE). The duration of treatment was 4 weeks. Swimming to exercise held 3 times a week and fructose solution dose was 270 grams for 9 days.Results -This research revealed that there was significantly difference on body weight before and after administration of fructose solution for 9 days (p=0.000). The lowest BDNF levels after treatment was group C and the highest of BDNF levels was group MI. There was no significantly difference in BDNF levels among groups after treatment (p=0.378), but there was tendency for BDNF levels to increase with exercise intensity. Conclusion-Swimming exercise with low-intensity, moderate-intensity, and high intensity has no effect on BDNF level in the hippocampus of fructose-induced mice.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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Exercise Intensity May affect Bdnf Level in the Hippocampus of Fructose-Induced Mice

Munir¹,

Miftahussurur²,

Pranoto³

et al. 2021

IJFMT

View full text Add to dashboard Cite

show abstract

“…This dose is capable of causing insulin resistance through the de novo lipogenesis process. The formation of fatty acids from excessive de novo lipogenesis can reduce insulin sensitivity, causing a decrease in glucose uptake by insulinsensitive tissue [13]. One protein on the cell surface playing an important role in the process of glucose uptake is GLUT4.…”

Section: Resultsmentioning

confidence: 99%

Ameliorative Effect of Carica papaya Seed Extract on Diabetic Rat Model with Muscle Atrophy

Wulansari

Wulandari

Risthanti

et al. 2019

MPI

Self Cite

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This study aims to prove that papaya seed extract has the potential to improve muscle cell atrophy in diabetic conditions. Type 2 diabetes mellitus (DM) animal model were made with conditions similar to type 2 DM in humans i.e. with long-term (56 days) high sugar (fructose) consumption. Then, the next 14 days were treated with papaya seed extract at the dose of 100, 200, and 300 mg/kg BW orally. Body weight and blood glucose levels were monitored throughout the study period. At the end of the study, histopathological examination of gastrocnemius skeletal muscle tissue was carried out using hematoxylin-eosin staining and measuring myocyte cell area. Results showed that there is a correlation between blood glucose levels with the area of muscle cells which explains that the decrease in blood glucose levels is in line with the increase in the area of muscle fiber cells. In the diabetic group and the treatment group the dose of 100 mg/kg BW has a smaller area, whereas in the treatment group the dose of 200 and 300 mg/kg BW has an area close to the area of the muscle cell in the normal group. This is supported by the results of measurements of the area of muscle fiber cells observed through cross sections and measured at the end of this study. The treatment group at the dose of 200 and 300 mg/kg BW did not have significant difference (P>0.05) compared to the normal group. It can be concluded that papaya seed extract therapy at the dose of 200 and 300 mg/kg BW on diabetic rats can reduce the fasting blood glucose levels so that it can ameliorate cell atrophy in the diabetic conditions.

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“…Fructose is the main regulator of glucose absorption and glycogen synthesis in the liver (Wulansari & Wulandari, 2018). High amounts of fructose can enter the hepatic circulation as a lipogenic agent and cause insulin resistance and glucose tolerance (Hidayati et al, 2020).…”

Section: Achievement Of Metabolic Syndrome Conditions In Experimental Animalsmentioning

confidence: 99%

POTENTIAL OF JAMBLANG (Syzygium cumini L.) LEAF EXTRACT ON BODY WEIGHT OF WISTAR RATS METABOLIC SYNDROME MODEL

Nurwati

Hermawan

et al. 2021

Jurnal Riset Kesehatan

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Jamblang (Syzygium cumini L.) leaves contain several phytochemical compounds. These phytochemicals are thought to have roles as antihyperglycemic, antihyperlipidemic, and antioxidant agents. This study aims to prove that there is an effect of giving ethanolic extract of jamblang leaves on body weight (BW) of metabolic syndrome (MS) Wistar rats induced by high-fat high-fructrose diet (HFFD) and injection of Streptozotocin (STZ)-Nicotinamide (Na), to determine different doses effect on the weight of Wistar rats. Laboratory experimental research with pre-post test control group. Samples were 8 weeks male Wistar (Rattus noverigicus) weighing 150-200 grams chosen by purposive random sampling method. Thirty rats were divided into 5 groups, each consisting of 6 rats. Normal group, metabolic syndrome group, 3 treatment groups MS were given jamblang leaf extract 100 mg/KgBW, 150mg/KgBW, 200mg/KgBW for 28 days. Data were analyzed by one way ANOVA test and repeated ANOVA test. The study showed an ethanolic extract of Jamblang leaves can reduce weight loss in Wistar rats with a metabolic syndrome model, and that the best dose used in this experiment is 150 mg/kgBW per day in Wistar rats with a metabolic syndrome model.

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Pengembangan Model Hewan Coba Tikus Diabetes Mellitus Tipe 2 dengan Induksi Diet Tinggi Fruktosa Intragastrik

Cited by 6 publications

References 4 publications

Exercise Intensity May affect Bdnf Level in the Hippocampus of Fructose-Induced Mice

Exercise Intensity May affect Bdnf Level in the Hippocampus of Fructose-Induced Mice

Ameliorative Effect of Carica papaya Seed Extract on Diabetic Rat Model with Muscle Atrophy

POTENTIAL OF JAMBLANG (Syzygium cumini L.) LEAF EXTRACT ON BODY WEIGHT OF WISTAR RATS METABOLIC SYNDROME MODEL

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