Pengaruh Bahan Organik Nabati dan Hewani terhadap Pertumbuhan Protocorm Like Bodies Phalaenopsis amabilis (L.) Blume

Yulianti, Yustia; Aisyah, Syarifah Iis; Sukma, Dewi

doi:10.29244/jhi.7.3.176-186

Cited by 5 publications

(7 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These concentrations were based on the results of researches that have been done previously. The use of bananas extracts by 100 g/L is recommended by Djanegara (2010), coconut water of 50 ml.L -1 is according to Nambiar et al (2012) and potato of 50 g/L is from Aisyah & Sukma (2016). The two factor experimental setup resulted in 16 treatment combinations with 3 replications each of which consisted of 3 samples.…”

Section: Methodsmentioning

confidence: 99%

Effect of IAA Addition and Some Organic Supplements on Growth and Rooting of Cavendish Banana (Musa Acuminata, AAA) In-Vitro

Sholikhah

Makhziah²,

Widiwurjani³

2022

JTEP-L

View full text Add to dashboard Cite

In vitro propagation of Cavendish banana is an alternative to produce healthy, uniform and sufficient plant materials of high qualities. Appropriate culture media formulation is needed for shoot growth and rooting stage. This experiment aimed to study effects of IAA and organic supplements on in vitro shoot growth and rooting. Explants were subjected to MS medium with IAA and various organic supplements, in a factorial arrangement of a completely randomized design. The first factor was IAA concentrations (0; 0.5; 1 and 1.5 mg.L-1), and the second factor was types of organic supplement (control, coconut water, potato extract and banana extract). At 8 weeks after planting, shoot growth and root performance of plantlets were recorded. The results showed that addition of IAA did not affect shoot height. Addition of potato extract and banana homogenate resulted in the highest plantlets. Medium with IAA at 1 mg.L-1 resulted in the highest number of roots. The highest number of shoots was produced on media with IAA 1 mg.L-1 + coconut water and IAA 1.5 mg.L-1 + potato extract. The highest number of roots was produced in medium with potato extract. The longest roots were produced in media with 0.5 mg.L-1 IAA + banana homogenate. Keywords: Banana extract, Cavendish, Coconut water, IAA, Potato extract

show abstract

Section: Methodsmentioning

confidence: 99%

Effect of IAA Addition and Some Organic Supplements on Growth and Rooting of Cavendish Banana (Musa Acuminata, AAA) In-Vitro

Sholikhah

Makhziah²,

Widiwurjani³

2022

JTEP-L

View full text Add to dashboard Cite

show abstract

“…The development of the best explants in the best media can increase the number of shoots [23]. In contrast, the administration of a higher concentration of fish emulsion will inhibit the division and regeneration of plant cells; in contrast, a high concentration of auxin can inhibit the division and regeneration of plant cells [24].…”

Section: Number Of Shootsmentioning

confidence: 99%

“…Chrysanthemum plants tend to grow better in purple sweet potato media than in other media treatments [8]. Fish emulsion produces optimal results of plantlet height, number of leaves, and root length of orchid protocorm-like bodies (PLBs) when roots emerge [9].…”

Section: Introductionmentioning

confidence: 99%

Organic materials and fish emulsion on shoot multiplication of Cavendish banana (Musa paradisiaca ‘Cavendish’)

Charantika

Samanhudi

Sakya

2022

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

This study aims to investigate the effect of combining organic matter and fish emulsion on the multiplication of Cavendish banana shoots. This study employed one factor of a complete randomized design, with 13 levels of treatment: MS (Murashige and Skoog) + purple sweet potato extract, MS + purple sweet potato extract + fish emulsion 2 ppm, MS + purple sweet potato extract + fish emulsion 4 ppm, MS + purple sweet potato extract + fish emulsion 6 ppm, MS + banana peel extract, MS + banana peel extract + fish emulsion 2 ppm, MS + banana peel extract + fish emulsion 4 ppm, MS + banana peel extract + fish emulsion 6 ppm, MS + bean sprouts extract, MS + Bean sprouts extract + fish emulsion 2 ppm, MS + bean sprouts extract + fish emulsion 4 ppm, MS + bean sprouts extract + fish emulsion 6 ppm, and MS + BAP (benzyl amino purine) as controls. The results show that the combination of synthetic materials (BAP) still has significantly different results compared to organic matter and fish emulsion treatment.

show abstract

“…Perkembangan morfologi tunas embrio Vanda hookeriana Rchb.f. di minggu ke-4, 8, dan 12 pada media perlakuan (kombinasi ekstrak pisang raja dan ekstrak yeast) (Yulianti, Aisyah, & Sukma, 2016). Pemberian ekstrak yeast fermipan, dan buah pisang raja dengan berbagai konsentrasi dalam media VW memberikan pengaruh yang berbeda-beda terhadap parameter-parameter pertumbuhan.…”

Section: Pengaruh Ekstrak Yeast Dan Pisang Raja Terhadap Morfologi Tunas Embrio Vanda Hookeriana Rchbfunclassified

“…Hasil menunjukkan bahwa perlakuan P3Y1 (pisang raja 150 g/L + ekstrak yeast 0,5 g/L) merupakan konsentrasi terbaik untuk semua parameter pertumbuhan akar yang diamati (jumlah akar, panjang akar, diameter akar, dan berat kering akar). Menurut Yulianti et al (2016) pemberian ekstrak buah pisang dapat berpengaruh nyata terhadap pertumbuhan akar anggrek bulan. Hasil ini juga sesuai dengan penelitian Santi et al (2011), yang menyebutkan bahwa pemberian ekstrak yeast pada anggrek Vanda berpengaruh nyata terhadap panjang akar dan jumlah akar.…”

Section: Jumlah Akar Panjang Akar Diameter Akar Dan Berat Kering Akarunclassified

Pengaruh Ekstrak Yeast dan Pisang Raja Terhadap Pertumbuhan Tunas Embrio Vanda hookeriana Rchb.f.

Rahayu

Utami

Indraloka

2021

Al-Kauniyah J. Biol.

View full text Add to dashboard Cite

AbstrakVanda hookeriana Rchb.f. merupakan anggrek yang dilindungi dan keberlangsungan hidupnya di alam terancam punah, sehingga perbanyakan anggrek secara in vitro sangat diperlukan. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak yeast dan pisang raja terhadap pertumbuhan tunas embrio anggrek V. hookeriana Rchb.f. Desain penelitian ini menggunakan RAL faktorial, faktor pertama adalah ekstrak yeast dengan 3 taraf: 0,5 g/L, 1 g/L, dan 1,5 g/L, faktor kedua adalah buah pisang raja dengan 3 taraf: 50 g/L, 100 g/L, dan 150 g/L. Penelitian dilakukan menggunakan teknik kultur jaringan, pengamatan dilakukan setiap bulan sekali dengan mengamati aspek morfologi plantlet. Media yang digunakan adalah Vacin-Went (VW) + kombinasi ekstrak yeast dan pisang raja. Parameter pengamatan berupa tinggi tunas, berat kering tunas, jumlah daun, diameter daun, panjang daun, berat kering daun, jumlah akar, diameter akar, panjang akar, berat kering akar, dan morfologi tunas. Hasil analisis menunjukkan bahwa perlakuan pisang raja 150 g/L + ekstrak yeast 0,5 g/L merupakan konsentrasi yang paling berperan terhadap parameter pertumbuhan anggrek V. hookeriana Rchb.f. Penelitian ini berhasil membuktikan bahwa penambahan komponen organik dalam media kultur jaringan memberikan respon pertumbuhan dan perkembangan yang baik pada eksplan. Komponen organik membantu proses fisiologis eksplan, karena komponen organik mengandung senyawa potensial yang dibutuhkan selama proses pertumbuhan dan perkembangan. Abstract Vanda hookeriana Rchb.f. is one of protected orchid and its survival in the wild was threatened by extinction, so orchid propagation was needed. This research aimed to determine the effect of yeast and pisang raja extracts to embryonal shoot growth of Vanda hookeriana Rchb.f. Experimental design used factorial RAL, first factor was yeast extract with 3 treatments (0.5 g/L; 1 g/L; & 1.5 g/L). Second factor was pisang raja with 3 treatments (50 g/L; 100 g/L; & 150 g/L). The research was conducted using in vitro culture techniques, observations were made once a month by observing the plantlet morphological parameters. The medium used was Vacin-Went (VW) + combination of yeast and pisang raja extracts. Observation parameters were shoot height, shoot dry weight, leaf number, leaf diameter, leaf length, leaf dry weight, number of roots, root diameter, root length, root dry weight and shoot morphology. The analysis showed that pisang raja treatment 150 g/L + yeast extract 0.5 g/L was the best concentration for the growth parameters of Vanda hookeriana Rchbf. This research were succeeded in proving that the addition of organic components in tissue culture media gave better response to growth and development of the explants. Organic components helps the explants in physiological ways because of the organic components contain potential compounds needed during the growth and development process.

show abstract

Pengaruh Bahan Organik Nabati dan Hewani terhadap Pertumbuhan Protocorm Like Bodies Phalaenopsis amabilis (L.) Blume

Cited by 5 publications

References 1 publication

Effect of IAA Addition and Some Organic Supplements on Growth and Rooting of Cavendish Banana (Musa Acuminata, AAA) In-Vitro

Effect of IAA Addition and Some Organic Supplements on Growth and Rooting of Cavendish Banana (Musa Acuminata, AAA) In-Vitro

Organic materials and fish emulsion on shoot multiplication of Cavendish banana (Musa paradisiaca ‘Cavendish’)

Pengaruh Ekstrak Yeast dan Pisang Raja Terhadap Pertumbuhan Tunas Embrio Vanda hookeriana Rchb.f.

Contact Info

Product

Resources

About