PENGARUH APLIKASI dsRNA VP-15 IN VITRO DAN IN VIVO TERHADAP SINTASAN DAN RESPONS IMUN UDANG WINDU Penaeus monodon

Parenrengi, Andi; Tenriulo, Andi; Mulyaningrum, Sri Redjeki Hesti; Lante, Samuel; Nawang, Agus

doi:10.15578/jra.14.4.2019.213-223

Cited by 3 publications

(5 citation statements)

References 23 publications

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“…A study of the dsRNA VP-24 vaccine at a dose of 0.2 μg showed a significant increase in survival rate in tiger shrimp by 65% compared to control (10%) (Mulyaningrum et al, 2018). Some studies also demonstrated that the intramuscular injection and oral delivery of dsRNA can provide better protection than control groups of tiger shrimp (Puneeth et al, 2017) and our studies indicated that the application of dsRNA by injection showed a better survival response than oral administration (Mulyaningrum et al, 2018;Parenrengi et al, 2018;2019). The shrimp survival by injected with dsRNA of VP24, VP19 and VP15 (Sarathi et al, 2010) and VP28 (Nilsen et al, 2017;Sarathi et al, 2008;Solis-Lucero et al, 2016) was significantly higher than that of control groups.…”

Section: Survival Ratesupporting

confidence: 54%

“…In contrast, Parenrengi et al (2019) reported that dsRNA vaccine had a significant effect on the proPO activity of tiger shrimp, where the highest proPO activity was obtained in the in-vivo VP15 dsRNA and subsequently invitro and the lowest in control (without dsRNA). This study also revealed that proPO activity increased the longer test animals were exposed to W SSV.…”

Section: Prophenoloxidase Activitymentioning

confidence: 89%

“…The successful production by invitro using MEGAscript RNAi kit and in-vivo (Mulyaningrum et al, 2018;Parenrengi et al, 2018) has been proven led to apply in aquaculture for preventing the WSD. Application of dsRNA produced by in-vivo showed better survival and immune response than control shrimp for both the injection and the immersion method (Parenrengi et al, 2018(Parenrengi et al, , 2019. The vaccination by oral administration is expected to be an efficient way in mass application.…”

Section: Introductionmentioning

confidence: 99%

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Application of dsRNA VP15-WSSV by Oral Vaccination to Increase Survival Rate and Response Immunes of Tiger Shrimp Penaeus monodon

Parenrengi

Tenriulo

Suryati

et al. 2022

IJAR

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Background: RNA interference (RNAi) has recently introduced as a powerful technique for specific gene silencing in antiviral therapy for controlling shrimp diseases. The present study was conducted to assess the effect of dsRNA VP15-WSSV vaccine by oral administration on tiger shrimp survival rate and response immunes. Methods: A gene encoding VP15 was isolated from tiger shrimp infected WSSV and cloned into the L4440 vector with the T7 promoter. For mass production, the pT7-VP15 gene construct was transformed to bacteria. The bacteria were inactivated using the heat-killed method by immersion and 108 cells of bacteria were mixed with 0.02 g of commercial shrimp feed for dsRNA application. The shrimp were fed by: (A) feed containing dsRNA and coated by fish oil, (B) feed without dsRNA and coated by fish oil and (C) feed without dsRNA and fish oil. The shrimp were challenged with WSSV by intramuscular injection after vaccination for two weeks. The survival rate (SR) after the challenge test was counted daily, while the THC and proPO were observed before, then on 1st, 3rd and 5th days post-challenge (dpc). Result: The survival rate of tiger shrimp for all treatments tended to decrease until the 3rd dpc, while the 4th dpc the vaccinated tiger shrimp was started to exhibit the higher SR compared to both treatments. The vaccinated tiger shrimp had significantly (P less than 0.05) higher SR and THC compared with both control treatments, but not significantly (P greater than 0.05) for proPO activity. The results indicated that the application of oral dsRNA vaccination suggested increasing in SR and response immune of tiger shrimp.

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Section: Survival Ratesupporting

confidence: 54%

Section: Prophenoloxidase Activitymentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

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Application of dsRNA VP15-WSSV by Oral Vaccination to Increase Survival Rate and Response Immunes of Tiger Shrimp Penaeus monodon

Parenrengi

Tenriulo

Suryati

et al. 2022

IJAR

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“…As a preliminary step, isolation and characterization of the coding genes VP-24 and VP-15 [15] and [16]has been carried out as well as their application in the form of recombinant DNA dsRNA, which has been constructed and produced in-vitro using mega script RNAi kits [17,18]. The optimal injection dose can be obtained in fighting white spot disease in tiger shrimp [19]. In-vivo production of dsRNA is carried out by cloning of the VP-WSSV gene in the RNAi vector and transformation in the competent of E. coli bacteria that can produce plasmids.…”

Section: Introductionmentioning

confidence: 99%

Application of dsRNA VP24 vaccine by oral administration at different larval stages of Tiger Shrimp Penaeus monodon

Tenriulo

Parenrengi

Lante

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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This study aimed to evaluate the effect of dsRNA VP24 application on different stages of larvae. For mass production, cloned recombinant bacteria carrying the construction of the promoter T7VP24 are planted in Luria Bertani broth medium. The bacteria were inactivated using the heat-killed bacteria method by immersion in water at 80 °C for 5 minutes. The inactivated bacteria were mixed with larval commercial feed. The nauplii were produced from broodstock from Aceh waters and reared until postlarva 12 (PL12). The feed containing the dsRNA vaccine was applied to a different stage of larva, i.e: starting from zoea 1 (A), mysis 1 (B), PL 1 to PL 12 (C), and control without dsRNA (D). The PL 12 were challenged with WSSV by the immersion method and morphological characters were assessed. Results showed that inactivating bacteria was effectively done by immersion method without damaging the dsRNA construct in the plasmid. The survival rate was significantly influenced by different stages of larvae (P 0.05), in which the highest survival (26.0%) was obtained from mysis. The highest value of morphological characters (92.3) was also inhibited in the mysis. The results suggested that the dsRNA vaccine for larvae could be started to be applied in the mysis stage.

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“…That issue made (Luo et al, 2003) conducted the first study with the transfer of the antiviral gene PmAV and the results showed that the transfer of antiviral genes could reduce the attack of WSSV pathogens on tiger shrimp. Parenrengi et al, (2019)…”

Section: Introductionmentioning

confidence: 99%

Increased Resistance of Penaeus Monodon to Whispovirus Causes White Spot Syndrome Through Antivirus Gene Transfer

Christanti

Widyanti

Rhenata

et al. 2021

BIOLINK

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The shrimp species Penaeus monodon is the main commodity of Indonesian aquaculture. One of the obstacles when cultivating tiger prawns is the attack of white spot disease (White Spot Syndrome Virus) which makes the quality and quantity of production decrease. One attempteffective for overcoming the white spot virus attack on shrimp, namely through the transfer of antiviral genes. Previous researchers carried out the transfer of antiviral genes to provide immunological effects so that they were resistant to pathogen attack and could prevent the death of tiger prawns. Destinationwriting article that is This study aims to determine the increase in resistance of tiger shrimp to Whispovirus that causes White Spot Syndrome through the transfer of antiviral genes. The type of research conducted is descriptive qualitative with a literature review method that discusses five focus studies, namely Whispovirus as a cause of White Spot Syndrome Virus (WSSV) disease in tiger prawns, tiger shrimp infected with White Spot Syndrome Virus, the use of PmAV antiviral genes in the treatment of Whispovirus , the mechanism of transfection of antiviral genes in the treatment of Whispovirus infection against tiger prawns, and changes in tiger shrimp resistance after insertion of antiviral genes. The conclusion obtained is that the addition of antiviral genes in tiger shrimp DNA can increase its resistance to Whispovirus.

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PENGARUH APLIKASI dsRNA VP-15 IN VITRO DAN IN VIVO TERHADAP SINTASAN DAN RESPONS IMUN UDANG WINDU Penaeus monodon

Cited by 3 publications

References 23 publications

Application of dsRNA VP15-WSSV by Oral Vaccination to Increase Survival Rate and Response Immunes of Tiger Shrimp Penaeus monodon

Application of dsRNA VP15-WSSV by Oral Vaccination to Increase Survival Rate and Response Immunes of Tiger Shrimp Penaeus monodon

Application of dsRNA VP24 vaccine by oral administration at different larval stages of Tiger Shrimp Penaeus monodon

Increased Resistance of Penaeus Monodon to Whispovirus Causes White Spot Syndrome Through Antivirus Gene Transfer

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