Penetration of 38% Hydrogen Peroxide into the Pulp Chamber in Bovine and Human Teeth Submitted to Office Bleach Technique

Camargo, Samira Esteves Afonso; Valera, Márcia Carneiro; Camargo, Carlos Henrique; Mancini, M; Menezes, Márcia Maciel

doi:10.1016/j.joen.2007.04.014

Cited by 126 publications

(115 citation statements)

References 28 publications

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“…The goal was to investigate whether the trans-enamel and transdentinal diffusion of a bleaching gel with high H 2 O 2 concentration widely used in in-office vital bleaching therapies, either catalyzed or not by halogen light, and the products of its degradation would cause potential toxic effects to pulp cell culture. Bovine teeth were used because their structural characteristics, such as number and diameter of dentinal tubules, are similar to those of human teeth (17). The thickness of the bovine enamel/dentin discs used in the present study (3.5 mm; 1.3 mm enamel and 2.2 mm dentin) was comparable to that described by Suleiman et al (4) for human teeth (0.9 to 1.05 mm enamel and 2.33 mm dentin).…”

Section: Discussionmentioning

confidence: 99%

Indirect cytotoxicity of a 35% hydrogen peroxide bleaching gel on cultured odontoblast-like cells

et al. 2009

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The aim of this study was to evaluate the trans-enamel and trans-dentinal effects of a 35% hydrogen peroxide (H 2 O 2 ) bleaching gel on odontoblast-like cells. Enamel/dentin discs obtained from bovine incisors were mounted in artificial pulp chambers (APCs). Three groups were formed: G1-35% H 2 O 2 ; G2-35% H 2 O 2 + halogen light application; G3-control. The treatments were repeated 5 times and the APCs were incubated for 12 h. Then, the extract was collected and applied for 24 h on the cells. Cell metabolism, total protein dosage and cell morphology were evaluated. Cell metabolism decreased by 62.09% and 61.83% in G1 and G2, respectively. The depression of cell metabolism was statistically significant when G1 and G2 were compared to G3. Total protein dosage decreased by 93.13% and 91.80% in G1 and G2, respectively. The cells in G1 and G2 exhibited significant morphological alterations after contact with the extracts. Regardless of halogen light application, the extracts caused significantly more intense cytopathic effects compared to the control group. After 5 consecutive applications of a 35% H 2 O 2 bleaching agent, either catalyzed or not by halogen light, products of gel degradation were capable to diffuse through enamel and dentin causing toxic effects to the cells.

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Section: Discussionmentioning

confidence: 99%

Indirect cytotoxicity of a 35% hydrogen peroxide bleaching gel on cultured odontoblast-like cells

et al. 2009

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“…Inconsistent recommendations were proposed regarding the use of bovine substrate as an alternative to human substrate in bonding/adhesive strength studies. Table 4 illustrates seven in vitro studies reviewed in this category (7,78,(82)(83)(84)(85)(86). Three of the seven studies found that microleakage values were higher in bovine compared to human substrates, both in enamel (7) and dentin (78,82).…”

Section: Dental Erosion/abrasion Studiesmentioning

confidence: 99%

“…On the other hand, three studies found no significant differences in marginal microleakage between human and bovine teeth, both in enamel (83)(84)(85) and dentin (83,84). Camargo et al (86) showed that the penetration of 38% hydrogen peroxide bleaching agent into pulp chambers was higher in human teeth compared to bovine teeth, regardless of the restorative material used.…”

Section: Dental Erosion/abrasion Studiesmentioning

confidence: 99%

Bovine teeth as substitute for human teeth in dental research: a review of literature

2011

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The aim of this paper was to review in vitro and in situ studies that directly compared the use of bovine teeth as a substitute for human teeth in dental experiments. A PubMed search was conducted for papers published from 1953 to December 30, 2010 using the following keywords: "human bovine enamel" or "human bovine dentin" or "human bovine teeth". The abstracts of the studies resulting from the keyword search were read, and all papers that compared human and bovine teeth were fully read. Only original articles written in English and directly comparing human and bovine substrates were included in the review. The search was supplemented by manual searches of the reference lists from each identified paper. Out of 76 studies initially selected, 68 fulfilled the selection criteria for inclusion. The studies covered seven categories: dental morphology, chemical composition, physical properties, dental caries, dental erosion/abrasion, bonding/adhesive strength, and marginal microleakage. Inconsistent data exist regarding whether bovine teeth can be considered an appropriate substitute for human teeth in dental research. Morphological, chemical composition and physical property differences between the two substrates must be considered when interpreting results obtained from any experiment using bovine tooth substrate. (J Oral Sci 53, 273-282, 2011)

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“…[1][2][3][4][5] In spite of this, most clinical studies have demonstrated that more than 70% of the patients who undergo in-office bleaching complain of tooth sensitivity (TS), 1,5,6 and this is the main deterrent to patients successfully completing their whitening treatment. Although the results of in vitro studies do not necessarily correlate to events that occur in an in vivo condition, one may hypothesize that the rapid transenamel and transdentinal diffusion of HP to the pulp, or other toxic components released with the degradation of the bleaching gels, 7,8 may be responsible for the high prevalence of TS. These by-products released from the bleaching gel act as free radicals and may cause oxidative stress in the pulp cells due to the imbalance between reactive oxygen species and endogenous and exogenous antioxidants.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of and Effect on Tooth Sensitivity of In-office Bleaching Gel Concentrations: A Randomized Clinical Trial

Reis

Kossatz

Martins

et al. 2013

Operative Dentistry

101

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A Reis S Kossatz GC Martins AD LoguercioClinical RelevanceFaster bleaching was observed with Whiteness HP Blue 35 (35% hydrogen peroxide) and tooth sensitivity was similar to that found with the lower concentration Whiteness HP Blue 20 (20% hydrogen peroxide). These results cannot be extrapolated to other in-office 35% hydrogen peroxide gels. SUMMARYWith the aim of reducing the side effects of inoffice bleaching agents, less-concentrated hydrogen peroxide (HP) gels have been released by manufacturers. We evaluated the tooth sensitivity (TS) and bleaching efficacy (BE) of two HP concentrations in this study. Gels containing 35% and 20% HP (HP35 and HP20, respectively) were applied on teeth of 60 caries-free patients. Color was recorded at baseline and one week after the first and second bleaching sessions using the Vita Classical shade guide. TS was recorded on a 0-4 scale. BE at each weekly recall was evaluated by Kruskall-Wallis and Mann-Whitney tests (a=0.05). Absolute risk of TS and its intensity was evaluated by Fisher exact and Mann-

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Penetration of 38% Hydrogen Peroxide into the Pulp Chamber in Bovine and Human Teeth Submitted to Office Bleach Technique

Cited by 126 publications

References 28 publications

Indirect cytotoxicity of a 35% hydrogen peroxide bleaching gel on cultured odontoblast-like cells

Indirect cytotoxicity of a 35% hydrogen peroxide bleaching gel on cultured odontoblast-like cells

Bovine teeth as substitute for human teeth in dental research: a review of literature

Efficacy of and Effect on Tooth Sensitivity of In-office Bleaching Gel Concentrations: A Randomized Clinical Trial

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