Penetration mechanism of dimethyl sulfoxide in human and pig ear skin: An ATR–FTIR and near-FT Raman spectroscopic<i>in vivo</i>and<i>in vitro</i>study

Grève, T.; Andersen, Kristine B.; Nielsen, Ole F.

doi:10.1155/2008/109782

Cited by 20 publications

(23 citation statements)

References 28 publications

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“…The first and the second band can be attributed to the α -helix and β -sheet structure, respectively. 46,47 The fact that the band at ~20° for the regenerated KER (purple curve) has the same intensity as that of the wool, but at ~9° it has only a broad shoulder instead of a pronounced band as in wool seems to indicate that regenerated KER has relatively lower α -helix contain and higher β -sheet, β -turn and random structure than wool. Similarly, the structure of two KER composites (25:75 CS:KER and 25:75 CEL:KER (green and blue curve)) is more similar to regenerated KER than wool, namely, relatively lower α -helix and higher β -sheet, β -turn and random structure.…”

Section: Resultsmentioning

confidence: 99%

Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties

Tran

Mututuvari

2016

ACS Sustainable Chem. Eng.

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A method was developed in which cellulose (CEL) and/or chitosan (CS) were added to keratin (KER) to enable [CEL/CS+KER] composites formed to have better mechanical strength and wider utilization. Butylmethylimmidazolium chloride ([BMIm+Cl−]), an ionic liquid, was used as the sole solvent, and because the majority of [BMIm+Cl−] used (at least 88%) was recovered, the method is green and recyclable. FTIR, XRD, 13C CP-MAS NMR and SEM results confirm that KER, CS and CEL remain chemically intact and distributed homogeneously in the composites. We successfully demonstrate that the widely used method based on the deconvolution of the FTIR bands of amide bonds to determine secondary structure of proteins is relatively subjective as the conformation obtained is strongly dependent on the choice of parameters selected for curve fitting. A new method, based on the partial least squares regression analysis (PLSR) of the amide bands, was developed, and proven to be objective and can provide more accurate information. Results obtained with this method agree well with those by XRD, namely they indicate that although KER retains its second structure when incorporated into the [CEL+CS] composites, it has relatively lower α-helix, higher β-turn and random form compared to that of the KER in native wool. It seems that during dissolution by [BMIm+Cl−], the inter- and intramolecular forces in KER were broken thereby destroying its secondary structure. During regeneration, these interactions were reestablished to reform partially the secondary structure. However, in the presence of either CEL or CS, the chains seem to prefer the extended form thereby hindering reformation of the α-helix. Consequently, the KER in these matrices may adopt structures with lower content of α-helix and higher β-sheet. As anticipated, results of tensile strength and TGA confirm that adding CEL or CS into KER substantially increase the mechanical strength and thermal stability of the [CS/CEL+KER] composites.

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Section: Resultsmentioning

confidence: 99%

Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties

Tran

Mututuvari

2016

ACS Sustainable Chem. Eng.

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“…[31][32][33] Dimethyl sulfoxide has been shown to change keratin conformation and also interact with SC lipids. 34,35) However, the interactions of these CPEs with SC lipids was mainly on the polar head region. 34,36,37) Therefore, low fluidity in the hydrophobic chain of SCLL lipids was observed in our study.…”

Section: Discussionmentioning

confidence: 99%

Potential of Stratum Corneum Lipid Liposomes for Screening of Chemical Skin Penetration Enhancers

Sakdiset

Todo

Sugibayashi

2017

CHEMICAL & PHARMACEUTICAL BULLETIN

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The evaluation of effective skin chemical penetration enhancers (CPEs) is a crucial process in the development of transdermal and dermal formulations with the capacity to overcome the stratum corneum barrier. In the present study, we aimed to investigate the potential of stratum corneum lipid liposomes (SCLLs) as an alternative tool for the screening of various types and concentrations of CPEs. SCLLs were prepared using a thin-film hydration technique, and two types of fluorescent probes ( Key words stratum corneum lipid liposome; chemical skin penetration enhancer; liposome; high-throughput screening Skin has been recognized as an application site of therapeutic drugs for several decades. However, only small numbers of drugs are available nowadays intended for systemic absorption through the skin. The stratum corneum (SC), the outermost layer of the skin, provides a strong barrier to protect against extraneous molecules from entering the body, and thus limits the skin penetration of drugs. The structure of the SC consists of keratin-rich corneocytes embedded in an intercellular lipid matrix.

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“…The amide I band provides information about the secondary structure of proteins in the skin [27]. An α-helix protein conformation gives rise to a band in the range of 1647 -1657 cm -1 while a β-sheet structure is associated with the 1621 -1640 cm -1 and 1671 -1679 cm -1 regions [28]. A shoulder near 1620 -1630 cm -1 was observed for both PG-and DMSO-treated samples.…”

Section: Atr-ftir Spectra For Tape Stripping Workmentioning

confidence: 99%

The application of ATR-FTIR spectroscopy and multivariate data analysis to study drug crystallisation in the stratum corneum

Goh

Craig

Hadgraft

et al. 2017

European Journal of Pharmaceutics and Biopharmaceutics

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Drug permeation through the intercellular lipids, which pack around and between corneocytes, may be enhanced by increasing the thermodynamic activity of the active in a formulation. However, this may also result in unwanted drug crystallisation on and in the skin. In this work, we explore the combination of ATR-FTIR spectroscopy and multivariate data analysis to study drug crystallisation in the skin.Ex vivo permeation studies of saturated solutions of diclofenac sodium (DF Na) in two vehicles, propylene glycol (PG) and dimethyl sulphoxide (DMSO), were carried out in porcine ear skin. Tape stripping and ATR-FTIR spectroscopy were conducted simultaneously to collect spectral data as a function of skin depth. Multivariate data analysis was applied to visualise and categorise the spectral data in the region of interest (1700 -1500 cm -1 ) containing the carboxylate (COO -) asymmetric stretching vibrations of DF Na. 3Spectral data showed the redshifts of the COO -asymmetric stretching vibrations for DF Na in the solution compared with solid drug. Similar shifts were evident following application of saturated solutions of DF Na to porcine skin samples. Multivariate data analysis categorised the spectral data based on the spectral differences and drug crystallisation was found to be confined to the upper layers of the skin.This proof-of-concept study highlights the utility of ATR-FTIR spectroscopy in combination with multivariate data analysis as a simple and rapid approach in the investigation of drug deposition in the skin. The approach described here will be extended to the study of other actives for topical application to the skin. GRAPHICAL ABSTRACT KEY WORDSStratum corneum; Crystallisation; ATR-FTIR; Multivariate data analysis, Porcine skin 4

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Penetration mechanism of dimethyl sulfoxide in human and pig ear skin: An ATR–FTIR and near-FT Raman spectroscopicin vivoandin vitrostudy

Cited by 20 publications

References 28 publications

Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties

Cellulose, Chitosan and Keratin Composite Materials: Facile and Recyclable Synthesis, Conformation and Properties

Potential of Stratum Corneum Lipid Liposomes for Screening of Chemical Skin Penetration Enhancers

The application of ATR-FTIR spectroscopy and multivariate data analysis to study drug crystallisation in the stratum corneum

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