Penetapan Kadar Flavonoid Dan Uji Aktivitas Antibakteri Ekstrak Sereh (Cymbopogon nardus) Dan Temu Kunci (Boesnbergia pandurata Roxb) Terhadap Bakteri Streptococcus Mutans

Abstract

Indonesia is rich in medicinal plants, including those that have the ability to inhibit bacterial growth, lemongrass (Cymbopogon nardus) and Fingerroots (Boesenbergia pandurata). Streptococcus mutans is a bacterium that causes dental caries. This Experiment want to determine the content of chemical compounds and the antibacterial activity of Lemongrass extract (Cymbopogon nardus) and Fingerroots (Boesenbergia pandurata) against Streptococcus mutans. The study was conducted experimentally with samples of lemongrass and Fingerroots. Extraction using maceration method using 70% Ethanol solvent. Phytochemical screening test with color test and determination of total flavonoid levels using a spectrophotometer. Antibacterial activity test uses the disk diffusion method against Streptococcus mutans. Phytochemical screening results of lemongrass extract and fingerroots with color test qualitative test showed positive containing flavonoids, saponins and tannins. In the test determination of total flavonoid levels, showed an average total flavonoid levels in lemongrass extracts of 48.61 mgQE / g and in Fingerroots extracts of 24.71 mgQE / g. The results of the antibacterial test against Streptococcus mutans obtained the largest average diameter of inhibitory zone in the extract of Fingerroots 5% w / v (11.167 mm), then the concentration of 5% w / v combination of lemongrass: Fingerroots 1: 2 (10.83 mm ), 2: 1 combination (10,067 mm). lemongrass (9.33 mm), 1: 1 combination (9,133 mm), Based on the Post Hoc test results that were comparable to positive controls were 5% fingerrots, combination of lemongrass: 1: 2 Fingerroots, and 2: 1 combination. The average total flavonoid level in lemongrass extract is more than the average total flavonoid content in Fingerroots. But in the same concentration 5% Fingerroots extract provides the greatest antibacterial inhibitory activities.

Identifikasi Metabolit Sekunder pada Sopi Kualin (SOKLIN) yang Dibuat Dengan dan Tanpa Fermentasi di Desa Kualin Nusa Tenggara Timur

“…Kemudian ditambahkan 1,42 g serbuk Mg dan 4 -5 tetes HCl pekat. Hasil positif jika terbentuk larutan merah atau jingga yang menunjukkan adanya flavonoid (Hati et al, 2019).…”

“…Selanjutnya, dimasukkan 1-2 mL H2SO4 pekat melalui dinding tabung reaksi. Jika hasil yang diperoleh berupa cincin kecoklatan atau violet pada perbatasan dua pelarut, maka sampel menunjukkan adanya triterpenoid sedangkan jika terbentuk warna hijau kebiruan maka sampel menunjukkan adanya steroid (Hati et al, 2019).…”

Potensi Rimpang Temu Kunci (Boesenbergia pandurata Roxb.) sebagai Bahan Aktif Produk Kecantikan Alami yang Ramah Lingkungan

“…Kombinasiutemuukunci dan serehhternyata tidak mampuimeningkatkan aktivitasmantibakteri dan serehhmengurangi aktivitaskantibakteri dari temuukunci [29]. Hal ini juga didukungioleh penelitian Hati dkk., (2019) [35], yang menunjukkan bahwa adanyaisenyawa panduratin dari rimpanggtemuikunci yang bersifat antibakteri terhadapiStaphylococcus. Sehingga, rimpang temuukunci berpotensi digunakan sebagaimbahan aktif dari sediaanmpasta gigimalami yang ramah lingkungan.…”

Anticaries Potential of Temu Kunci-Serai Ethyl Acetate Extract Combination: In Vitro and Molecular Studies Approach

“…Temu kunci and serai on their way can be used as antibacterial Streptococcus mutans [10][11][12] . Previous combination research has been conducted between temu kunci and serai which has the potential as an anticaries [13,14] . Ethyl acetate was used as a solvent according to research conducted by Elfahmi et al [15] and Priyadi et al [16] .…”

“…Inhibition zone (mm) Category [27] *Reference for comparing [13] The value of tuckey analysis showed a significant difference between the positive control treatment, negative control, and the three combinations of temu kunci-serai while there was no significant difference between the negative control and temu kunci-serai due to the small inhibition zone value.…”