PENETAPAN KADAR FENOLIK TOTAL EKSTRAK METANOL KELOPAK BUNGA ROSELLA MERAH (Hibiscus sabdariffa Linn) DENGAN VARIASI TEMPAT TUMBUH SECARA SPEKTROFOTOMETRI

Alfian, Riza; Susanti, Hari

doi:10.12928/pharmaciana.v2i1.655

Cited by 35 publications

(48 citation statements)

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“…The Folin Ciochalteau reagent is used because the phenolic compound forms a blue color complex compound which can be measured at a wavelength (alkali salt) or a hydroxy phenol group reducing the heteropoly acid (fosfomolibdat-phosphotungstat) contained in the folin ciocalteau reagent into a blue molybdenum-tungsten complex. Phenolic compounds only in alkaline atmosphere using Na2CO3 7.5% proton dissociation into phenolic ions, the greater the concentration of phenolic compounds the more the phenolic ions that reduce the heteropolyacids into molybdenum-tungsten complex so the blue color becomes thicker [9].…”

Section: Resultsmentioning

confidence: 99%

Analysis of Total Phenolic From a Root Tabar Kedayan

Jubaidah

Nurhasnawati

Wijaya

2019

J. Trop. Pharm. Chem.

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East Kalimantan has a diversity of plants from various Dayak ethnic groups. One of them is the Tabar Kedayan (Aristolochia foveolata Merr) plant located in Malinau district. This plant is empirically efficacious as antidiarrhea, antidotum (anti poison). Efficacy of Tabar Kedayan plant has not been scientifically confirmed, this is because there is still little scientific research and information about the content of secondary metabolites and bioactive compounds contained in Tabar Kedayan Root. The presence of secondary metabolite is phenolic which has considerable biological active prospects as antioxidant, antibacterial, antiamuba, anti-inflammatory, antihepatotoxic and antivirus. The aim of this research is to analyze the chemical content and total phenolic content of root tabar kedayan in fractionation with various nonpolar, semipolar and polar solvents. The analysis used in the determination of total phenolic content using visible spectrophotometric method. Data of analysis used standard curve method based on absorbance data and concentration of standard solution. The results of this study obtained the highest total phenolic average on ethyl acetate fraction of 77.74 ±2,633 mg GAE/g then ethanol fraction of water amounted to 38.10 ±0,461 mg GAE/g and the smallest level of n-hexane fraction of 29.36±0,193 mg GAE/g. Conclusions in this study total phenolic content is found in most semipolar fractions.

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Section: Resultsmentioning

confidence: 99%

Analysis of Total Phenolic From a Root Tabar Kedayan

Jubaidah

Nurhasnawati

Wijaya

2019

J. Trop. Pharm. Chem.

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“…Penambahan Na2CO3 bertujuan agar fenolik dapat beraksi dengan reagen Folin-Ciocalteu. Senyawa fenolik hanya akan bereaksi dengan reagen Folin-Ciocalteu dalam suasana basa agar terjadinya suatu disosiasi proton antara senyawa fenolik menjadi ion fenolat dengan adanya penambahan larutan Na2CO3 [28].…”

Section: Tabel 3 Hasil Pengukuran Kadar Total Fenolikunclassified

Skrining Fitokimia dan Penetapan Kandungan Total Fenolik Ekstrak Daun Tumbuhan Sapu-Sapu (Baeckea frutescens L.)

et al. 2020

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Tumbuhan sapu-sapu (Baeckea frutescens L.) merupakan salah satu jenis keanekaragaman hayati yang tumbuh dan persebarannya cukup banyak di Indonesia. B. frutescens L diketahui memiliki senyawa metabolit sekunder aktif yang dapat dimanfaatkan sebagai obat, antibakteri, dan antioksidan. Penelitian ini dilakukan untuk menganalisis kandungan senyawa metabolit sekunder yang terdapat pada B. frutescens L. Metode yang digunakan adalah ekstraksi dilanjutkan dengan pengujian fitokimia kualitatif yang terdiri dari tujuh pengujian yakni uji fenol, tanin, flavonoid, saponin, alkaloid, steroid dan terpenoid serta pengujian kuantitatif yakni pengujian total fenolik ekstrak B. frutescens L. Hasil penelitian menunjukkan bahwa didapatkan persentase bobot rendemen ekstrak daun B. frutescens L. sebesar ekstrak n-heksan 5,39%, ekstrak etil asetat 14,54% dan ekstrak etanol yakni 19,81%. Hasil pengujian fitokimia kualitatif menujukkan senyawa fitokimia yang terkandung di dalam tumbuhan B. frutescens L. yakni pada ekstrak n-heksan hanya terdapat senyawa steroid dan pada ekstrak etil asetat terdapat senyawa fenolik, tanin, flavonoid, dan alkaloid. Selain itu, pada ekstrak etanol terdapat senyawa fenolik, tanin, flavonoid, saponin, steroid, dan alkaloid. Pengujian total fenolik ekstrak daun tumbuhan B. frutescens L. untuk pelarut etil asetat yakni 0,24% dan pelarut etanol yakni sebesar 0,14% dihitung terhadap senyawa fenol asam galat. Hasil penelitian ini diharapkan dapat menjadi informasi untuk penelitian lebih lanjut mengenai pemanfaatan kandungan metabolit sekunder B. frutescens L.

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“…Ekstrak 100 mg dimasukkan ke dalam tabung reaksi, kemudian ditambahkan dengan pelarut metanol secukupnya, lalu ditambahkan 3 tetes larutan FeCl 3 . Terbentuknya warna hijau, merah, ungu, biru, dan hitam yang kuat menunjukkan adanya senyawa fenol dalam bahan (Alfian & Susanti, 2012).…”

Section: Uji Fenolikunclassified

“…Prinsip dari metode folin-ciocalteau adalah terbentuknya senyawa kompleks berwarna biru yang dapat diukur pada panjang gelombang maksimal. Pereaksi ini mengoksidasi fenolat (garam alkali) dan gugus fenolik-hidroksi mereduksi asam heteropoli (fosfomolibdat-fosfotungstat) yang terdapat dalam pereaksi Folin-ciocalteau menjadi suatu kompleks molybdenum-tungsten (Alfian & Susanti, 2012). Senyawa fenolik bereaksi dengan reagen hanya dalam suasana basa agar terjadi disosiasi proton pada senyawa fenolik menjadi ion fenolat, sehingga ditambahkan larutan Na 2 CO 3 (Apsari et al, 2011).…”

Section: Hasil Dan Pembahasanunclassified

Pengaruh Metode Ekstraksi Terhadap Rendemen Dan Kadar Fenolik Ekstrak Tanaman Kayu Beta-Beta (Lunasia amara Blanco)

Hasnaeni

usman

Wisdawati

2019

JFG

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Lunasia amara Blanco bark belonging to the family Rutaceae. The research aimed to determine the effect of extraction method on yield value and phenolic content of bark extract of Lunasia amara Blanco by UV-Vis spectrophotometry. Sample was extracted by maceration, reflux, and soxhlet using methanol 70%. The extraction by maceration obtained the yield value of methanol extract of 2,352%; the reflux method of 1,611%; and the soxhlet method of 0,960%. Then the chemical content was identified by the addition of FeCl3 reagent and a blackish green color was formed positively indicating phenolic content. Afterward, phenolic identification was conducted by TLC with eluent n-hexane: ethyl acetate (2:8) containing phenolic because the Rf value was the same as the galic acid standard. The results showed that the extraction method producing the highest yield of bark extract of Lunasia amara Blanco was maceration. The phenolic content obtained by maceration was 66,548 mgGAE/g extract with 6.6548% GAE; the reflux was 73.645 mgGAE/g extract with 7.3645% GAE; and the soxhlet was 74.806 mgGAE/g extract with 7.4806% GAE.

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PENETAPAN KADAR FENOLIK TOTAL EKSTRAK METANOL KELOPAK BUNGA ROSELLA MERAH (Hibiscus sabdariffa Linn) DENGAN VARIASI TEMPAT TUMBUH SECARA SPEKTROFOTOMETRI

Abstract: Abstrak

Cited by 35 publications

References 0 publications

Analysis of Total Phenolic From a Root Tabar Kedayan

Analysis of Total Phenolic From a Root Tabar Kedayan

Skrining Fitokimia dan Penetapan Kandungan Total Fenolik Ekstrak Daun Tumbuhan Sapu-Sapu (Baeckea frutescens L.)

Pengaruh Metode Ekstraksi Terhadap Rendemen Dan Kadar Fenolik Ekstrak Tanaman Kayu Beta-Beta (Lunasia amara Blanco)

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