Analysis of Total Phenolic From a Root Tabar Kedayan

Abstract

East Kalimantan has a diversity of plants from various Dayak ethnic groups. One of them is the Tabar Kedayan (Aristolochia foveolata Merr) plant located in Malinau district. This plant is empirically efficacious as antidiarrhea, antidotum (anti poison). Efficacy of Tabar Kedayan plant has not been scientifically confirmed, this is because there is still little scientific research and information about the content of secondary metabolites and bioactive compounds contained in Tabar Kedayan Root. The presence of secondary metabolite is phenolic which has considerable biological active prospects as antioxidant, antibacterial, antiamuba, anti-inflammatory, antihepatotoxic and antivirus. The aim of this research is to analyze the chemical content and total phenolic content of root tabar kedayan in fractionation with various nonpolar, semipolar and polar solvents. The analysis used in the determination of total phenolic content using visible spectrophotometric method. Data of analysis used standard curve method based on absorbance data and concentration of standard solution. The results of this study obtained the highest total phenolic average on ethyl acetate fraction of 77.74 ±2,633 mg GAE/g then ethanol fraction of water amounted to 38.10 ±0,461 mg GAE/g and the smallest level of n-hexane fraction of 29.36±0,193 mg GAE/g. Conclusions in this study total phenolic content is found in most semipolar fractions.

Perbandingan Kadar Fenolik Total Air Rebusan dan Ekstrak Etanol Daun dan Batang Sawi Langit (Cyanthillium cinereum (L.) H.Rob)

“…Penelitian ini menggunakan daun dan batang sawi langit, karena belum diketahui pada bagian manakah yang banyak mengandung senyawa fenolik, untuk penetapan kadar fenol daun dan batang tersebut menggunakan pelarut air dan etanol yang sifatnya sama-sama polar sesuai dengan senyawa fenol, kedua pelarut tersebut digunakan untuk membandingkan senyawa fenol yang lebih tinggi diantara daun dan batang (Tarasevičienė, Vitkauskaitė, Paulauskienė, & Černiauskienė, 2023). Penetapan total kadar suatu senyawa seperti fenolik dilakukan agar pemanfaatan tumbuhan sebagai pengobatan lebih baik, sehingga semakin efektif senyawa yang digunakan dalam uji aktivitas (Jubaidah et al, 2019).…”