The study aimed to determine the level of Moringa oleifera (MO) flour to maintain the performance of sapi pejantan Peranakan Ongole as fresh semen producers. The feed given has a maximum content of DM 3% BW and CP 11%. The study used eight PO bull with an initial body weight of 558.63±127.29 kg aged 3-5 years. The experimental design was designed using two treatments with four replications. The treatment given was vitamin A, E, mineral Zinc, and 100 grams/head/day of MO flour supplementation (P1) and vitamin A, E, mineral Zinc, and 50 grams/head/day of MO flour supplementation (P2). The parameters observed were feed consumption (dry matter, crude protein, crude fiber, total digestible nutrient (TDN)), average daily gain (ADG), body condition score (BCS), linear body (hearth girth, body length, wither height), and scrotum circumference. Data were analyzed using the t-test. The results showed that there were no significant differences between the two levels of MO flour giving to feed consumption (DM, CP, CF, TDN, ADG, BCS, linear body (hearth girth, body length, wither height), and scrotum circumference. Decreases in BCS values and scrotum circumference were seen in P1, whereas in P2 showed positive growth in all body size and scrotum circumference parameters. It was concluded that P2 could be used as supplementation to have a positive impact on the scrotum circumference without a decrease in the production performance and linear growth of body weight of PO bulls.