Penaeus monodon larvae can be protected from Vibrio harveyi infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics

Abstract

This study shows that the disease resistance and survival rate of Penaeus monodon in a larval rearing systems can be enhanced by supplementing with antagonistic or non-antagonistic probiotics. The antagonistic mode of action of Pseudomonas MCCB 102 and MCCB 103 against vibrios was demonstrated in larval mesocosm with cultures having su⁄cient concentration of antagonistic compounds in their culture supernatant. Investigations on the antagonistic properties of Bacillus MCCB 101, Pseudomonas MCCB 102 and MCCB 103 and Arthrobacter MCCB 104 against Vibrio harveyi MCCB 111under in vitro conditions revealed that Pseudomonas MCCB 102 and MCCB 103 were inhibitory to the pathogen. These inhibitory properties were further con¢rmed in the larval rearing systems of P. monodon. All these four probionts signi¢cantly improved larval survival in long-term treatments as well as when challenged with a pathogenic strain ofV. harveyi MCCB111. We could demonstrate that Pseudomonas MCCB 102 and MCCB 103 accorded disease resistance and a higher survival rate in P. monodon larval rearing systems through active antagonism of vibrios, whereas Bacillus MCCB 101 and Arthrobacter MCCB 104 functioned as probiotics through immunostimulatory and digestive enzyme-supporting modes of action.

Antagonistic effect of Pseudomonas aeruginosa isolates from various ecological niches on Vibrio species pathogenic to crustaceans

“…To determine the extent of salinity tolerance and preference of all the isolates of P. aeruginosa and the influence of salinity on their growth and antagonistic compound production, the isolates were individually inoculated into 100 mL media containing 0.5% peptone, 0.1% yeast extract (pH 7.5) at varying salinities of 5,10,15,20,30,40,50,60, and 70 g/L obtained by adding sea water. For obtaining salinity above 30 g/L NaCl was added to seawater.…”

“…were grown on ZoBell's marine agar slants (2216E HiMedia, India) and incubated at (28依1) °C overnight. These cultures were harvested in sterile 15 g/L salinity seawater, absorbance (Abs 600 ) adjusted to 1.5 and swabbed 500 µL onto ZoBell's marine agar plates. The isolates of Pseudomonas were grown in the above mentioned media and incubated at (28依1) °C .…”

“…have been gaining increasing attention as biological control agents against pathogenic fungi and bacteria in agriculture [2][3][4] , vibrios in aquaculture [5,6] and also as bioaugmenters in bioremediation programs [7][8][9] . Specifically, the versatile and ubiquitous bacterium, Pseudomonas aeruginosa [10,11] ( P. aeruginosa ) has recently been recognized as an active antagonist of pathogenic Vibryo harveyi (V. harveyi) and thus as a candidate probiotic in aquaculture systems [12][13][14][15] . The anti-vibrio compound produced by P. aeruginosa was proved to be pyocyanin [16] , a blue-green chloroform-soluble phenazine pigment, which possessed broad-spectrum antibacterial [17] , anti-fungal [18,19] and antiprotozoan activities [20] .…”

The fish egg microbiome : diversity and activity against the oomycete pathogen Saprolegnia

“…To combat common and emerging aquatic diseases, a comprehensive understanding of the dynamics, richness, abundance and functions of microbiomes associated with their aquatic hosts and environment is pivotal to obtain the right bioaugmentation candidates for application. et al, 1983;Gibson, 1998;Gibson et al, 1998;Irianto and Austin, 2002a, b;Brunt and Austin, 2005;Pieters et al, 2008; Agarivorans (Douillet and Langdon, 1993;Douillet and Langdon, 1994;Kesarcodi-Watson et al, 2009KesarcodiWatson et al, 2012) Arthrobacter (Jayaprakash et al, 2005;Salonius et al, 2005;Plante et al, 2007;Li et al, 2008;Pai et al, 2010) Kennedy et al, 1998;Hirata et al, 1998;Moriarty, 1998;Queiroz and Boyd, 1998;Rengpipat et al, 1998;Raida et al, 2003;Rengpipat et al, 2003;…”

“…Survival, immune responses (Balcázar et al, 2006;Balcázar et al, 2007b;Balcázar et al, 2007a;Luis Balcazar et al, 2007;Vendrell et al, 2008) Microbacterium Ruiz-Ponte et al, 1998;Ruiz-Ponte et al, 1999;Hjelm et al, 2004;Martens et al, 2006;Porsby et al, 2008;Kesarcodi-Watson et al, 2012;D'Alvise et al, 2013) (Gram et al, 1999;Vijayan et al, 2006;Gobeli et al, 2009;Hai et al, 2009;Aguilar-Macías et al, 2010;Pai et al, 2010;Campa-Córdova et al, 2011;Giri et al, 2011;Korkea-aho et al, 2011;Giri et al, 2012;Korkea-aho et al, 2012; Rhodopseudomonas Varela et al, 2010;<...>…”