Antagonistic effect of Pseudomonas aeruginosa isolates from various ecological niches on Vibrio species pathogenic to crustaceans

doi:10.12980/jclm.2.2014j30

Cited by 7 publications

(6 citation statements)

References 28 publications

(55 reference statements)

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“…On this account, P. aeruginosa could be considered an important species with high metabolic diversity having ecological and clinical significance [17,18,29]. Their structurally diverse secondary metabolites are already known to have inhibitory potential against aquaculture pathogens especially belonging to Vibrio and Aeromonas [3,30,31]. Thus P. aeruginosa Ps04 can be well explored to be used as an antibacterial agent in aquaculture.…”

Section: Discussionmentioning

confidence: 99%

“…The optimal physiochemical conditions for growth and production of the antibacterial compound by P. aeruginosa Ps04 were assessed by growing the culture in the optimized glycerol alanine medium at various temperatures (25,30,35 and 40°C), pH (6.0, 7.0, 8.0 and 9.0), salinity (5, 10, 15, 20, 25, 30 and 35 ppt), carbon sources (glycerol and glucose at varying concentrations from 0.5-1%) and nitrogen sources (alanine and glutamic acid at varying concentrations from 0.5-1%) for a period of 7 days (at 30°C at 200 rpm) in duplicates. Samples for cell count and antibacterial activity were drawn at every 24 h.…”

Section: Microbial Strainsmentioning

confidence: 99%

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Antibacterial assay guided isolation of a novel hydroxy-substituted pentacyclo ketonic compound from Pseudomonas aeruginosa MBTDCMFRI Ps04

Nair

K²,

et al. 2020

Braz J Microbiol

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Antimicrobial compounds from the natural source have gained greater relevance because of their wide spectrum of possible applications, especially in the aquaculture industry where pathogenic threat and antibacterial resistance are serious concerns. In this regard, Pseudomonas aeruginosa MBTDCMFRI Ps04 (P. aeruginosa Ps04) strain isolated from the tropical estuarine habitats of Cochin was evaluated for its antibacterial potential against major aquaculture pathogens. The physiological conditions for the maximum production of the active metabolite were also optimized. An activity-guided approach was employed further to isolate and characterize the secondary metabolite responsible for the inhibitory potential. It was found that the cell free supernatant (CFS) of P. aeruginosa Ps04 exhibited strong antibacterial activity against major aquaculture pathogens belonging to the genus Vibrio and Aeromonas and retained its potential even at 30% (v/v) dilution. The highest antibacterial activity was detected from 3rd day culture, grown in glycerol alanine media (1% each) as carbon and nitrogen source, respectively, at 30°C, pH 7.0 and at a salinity of 20 parts per thousand (ppt). The activity of the antagonistic principle was found to be stable against variations in pH (pH 2-pH 12), temperature (up to 120°C) and enzymatic treatments. Bioassay-guided purification followed by spectroscopic characterization of active fractions of P. aeruginosa Ps04 revealed that the compound 4-Hydroxy-11-methylpentacyclo [11.8.0.0 2,3 .0 11, 12 .0 16,17 ]henicosa-1,3,5,8(9),17-penten-14-one is responsible for its major antibacterial activity. The results of this study indicated that P. aeruginosa Ps04 has beneficial antibacterial properties which could be used in developing novel antimicrobial therapeutics against a variety of aquaculture pathogens.

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Section: Discussionmentioning

confidence: 99%

Section: Microbial Strainsmentioning

confidence: 99%

Antibacterial assay guided isolation of a novel hydroxy-substituted pentacyclo ketonic compound from Pseudomonas aeruginosa MBTDCMFRI Ps04

Nair

K²,

et al. 2020

Braz J Microbiol

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“…Isolation of extract fermentation led to identify pyocyanin (26) as the bioactive compound responsible for the antagonistic effect at a concentration of more than 30 mg L −1 . 65 Pseudomonas aeruginosa PA31X produces phenazine-1-carboxylic acid ( 27) that is active against V. anguillarum C312 at 3 mg mL −1 . 66…”

Section: Compoundsmentioning

confidence: 99%

Natural products as antivibrio agents: insight into the chemistry and biological activity

2022

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“…This antagonistic property of pyocyanin to various pathogenic microorganisms have been studied extensively 3,7,89,142 . The application of pyocyanin pigment at concentration 5-10 mg/mL does not show any deleterious or pathological effect in the eukaryotic system and can be used as a biocontrol agent against fungal and bacterial pathogens in agriculture and aquaculture against Vibrios 137 .…”

Section: Applications Of Pyocyaninmentioning

confidence: 99%

“…It has been used to control phytopathogens 157 . In addition to this, as pyocyanin pigment is water-soluble, it has also been reported for its application in aquaculture 137 . Pyocyanin pigment can generate reactive oxygen species (ROS) and these ROS interferes with the topoisomerase I and II activities in eukaryotic cells (tumor cells) 58 .…”

Section: Introductionmentioning

confidence: 96%

Challenges and Opportunities with Pyocyanin Pigment of Pseudomonas aeruginosa

Rani,

Azmi

2020

J. Adv. Mircobiol.

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Microbial secondary metabolites are the organic compounds that are not directly involved in the normal growth, development, or reproduction of an organism but very useful for human health. These are formed during the stationary phase (idiophase) of culture, also known as idiolites such as pigments, toxins, alkaloids, antibiotics, etc, and have a very unusual structure. Production of secondary metabolites occurred when there is depletion of one or more nutrients in the culture medium. Microbial pigments such as carotenoids, melanin, prodigiosin, violacein, pyocyanin, etc, are natural colors that hold promising potential to meet various challenges in the pharmaceutical and nutraceutical sector. Pyocyanin is one of many natural pigments, a blue-colored redox-active secondary metabolite, which is produced by Pseudomonas aeruginosa. Several virulence factors are secreted by P. aeruginosa that can contribute to its pathogenicity and pyocyanin pigment is one of them. It causes oxidative stress to the host, disrupting host catalase, mitochondrial electron transport, induces apoptosis in neutrophils as well as inhibits the phagocytosis of apoptotic bodies by macrophages. It is challenging for anti-virulence drug intervention to control the pathogenesis of P. aeruginosa to some extent by targeting the biosynthetic pathway of pyocyanin. Besides this, pyocyanin pigment possesses antioxidant, antimicrobial, anti-inflammatory, anticancerous, and immunosuppressive properties. This article aims to describe challenges posed by pyocyanin pigment of P. aeruginosa and its potential applications in various fields of biotechnology.

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Antagonistic effect of Pseudomonas aeruginosa isolates from various ecological niches on Vibrio species pathogenic to crustaceans

Cited by 7 publications

References 28 publications

Antibacterial assay guided isolation of a novel hydroxy-substituted pentacyclo ketonic compound from Pseudomonas aeruginosa MBTDCMFRI Ps04

Antibacterial assay guided isolation of a novel hydroxy-substituted pentacyclo ketonic compound from Pseudomonas aeruginosa MBTDCMFRI Ps04

Natural products as antivibrio agents: insight into the chemistry and biological activity

Challenges and Opportunities with Pyocyanin Pigment of Pseudomonas aeruginosa

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