Pembentukan Formaldehid Alami pada Beberapa Jenis Ikan Laut selama Penyimpanan dalam Es Curai

Murtini, Jovita Tri; Riyanto, Rudi; Priyanto, Nandang; Hermana, Irma

doi:10.15578/jpbkp.v9i2.107

Cited by 14 publications

(28 citation statements)

References 7 publications

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“…Beberapa penelitian menunjukkan fakta bahwa formaldehid terbentuk secara alami pada ikan (Riyanto et al 2006;Murtini et al 2014;Bianchi et al 2007) hal ini mengindikasikan perlunya penafsiran tentang penetapan kandungan formalin (formaldehid) dalam peraturan yang ditetapkan, karena dalam kenyataanya formaldehid dapat terbentuk selama proses kemunduran ikan.…”

Section: Pendahuluanunclassified

“…Perubahan mutu setelah ikan mati dapat disebabkan oleh aktivitas enzim, biokimia, fisik, mikrobiologi dan pembentukan formaldehid secara alami dapat berlangsung selama proses pembusukan (Murtini et al 2014). menyatakan bahwa formaldehid alami merupakan produk dekomposisi enzimatik dari trimetilamin oksida (TMAO) pada ikan selama penyimpanan post-mortem dengan hasil samping dimetilamin.…”

Section: Pendahuluanunclassified

“…Murtini et al (2004) Hasil analisis kadar formaldehid dari beberapa penelitian menunjukkan hasil yang berbeda-beda kadar formaldehid cumi-cumi yang disimpan pada suhu chilling selama 15 hari adalah 8,37 ppm (Apandi 2017), kadar formaldehid ikan kembung yang disimpan pada suhu chilling selama 18 hari adalah 9,95 ppm (Sahliyah 2017). Kadar formaldehid selama penyimpanan 12 hari pada bawal bintang sebesar 0,95 ppm, kakap putih sebesar 1,5 ppm, bandeng sebesar 0,715 ppm, kakap merah sebesar 1,381 ppm dan kerapu cantrang sebesar 1,303 ppm (Murtini et al 2014). Hal ini menunjukkan bahwa kandungan formaldehid dipengaruhi oleh perbedaan spesies.…”

Section: Hasil Dan Pembahasan Kadar Formaldehid Ikan Beloso Selama Peunclassified

“…Suhu penyimpanan yang semakin rendah maka peningkatan formladehidnya semakin kecil. Murtini et al (2014) menyatakan bahwa suhu rendah dapat menghambat penurunan mutu ikan yang terlihat dari kandungan formaldehid yang terbentuk masih dalam level yang rendah. Hal ini disebabkan karena aktivitas enzim yang menguraikan TMAO sedikit terhambat karena penggunaan suhu rendah.…”

Section: Hasil Dan Pembahasan Kadar Formaldehid Ikan Beloso Selama Peunclassified

“…Dimetilamin secara alami terbentuk sebagai hasil samping reduksi trimetilamin oksida (TMAO) oleh enzim endogenus yang juga akan menghasilkan formaldehid (Murtini et al 2014). Hasil analisis kandungan dimetilamin pada ikan beloso selama penyimpanan suhu chilling disajikan pada Gambar 2.…”

Section: Kadar Dimetilamin Kadar Dimetilamin Ikan Beloso Selama Penyiunclassified

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Activity of Trimethylamine-N-Oxide Demethylase (TMAOase) in the Forming of Natural Formaldehyde in Lizardfish (Saurida tumbil)

2017

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Cara sitasi: Nuraini A, Nurhayati T, Nurilmala M. 2017. Aktivitas trimethylamine-n-oxide demethylase (TMAOase) dalam pembentukan formaldehid alami pada ikan beloso (Saurida tumbil). Jurnal Pengolahan Hasil Perikanan Indonesia. 20(3): 549-558. AbstrakPembentukan formaldehid pada tubuh ikan dapat terjadi secara alami melalui kerja enzim trimethylamine-N-Oxide demethylase (TMAO)ase yang memecah TMAO menjadi formaldehid dan dimetilamin. Pembentukan formaldehid pada ikan dapat terjadi selama penyimpanan. Penelitian ini bertujuan untuk menentukan aktivitas TMAOase dan perannya dalam pembentukan dimetilamin dan formaldehid alami ikan beloso (Saurida tumbil) segar yang disimpan pada suhu chilling. Pengamatan dilakukan setiap 2 hari selama 16 hari penyimpanan. Analisis yang dilakukan yaitu kandungan formaldehid, dimetilamin, dan aktivitas spesifik enzim TMAOase. Kandungan formaldehid mengalami peningkatan selama penyimpanan yaitu 0,22±0,00 ppm pada hari ke-0 menjadi 7,45±0,46 ppm pada hari ke-16. Hasil uji dimetilamin juga meningkat selama penyimpanan dari 0,93±0,50 ppm pada hari ke-0 menjadi 27,26±1,8 ppm pada hari ke-16. Hasil pengujian aktivitas spesifik TMAOase ikan beloso meningkat selama penyimpanan yaitu 1,15 U/mg pada hari ke-0, menjadi 16,47 U/mg pada hari ke-16. Kata kunci: dimetilamin, formaldehid, ikan beloso, suhu chilling, trimethylamine-N-Oxide demethylase Activity of Trimethylamine-N-Oxide Demethylase (TMAOase) in the Forming of Natural Formaldehyde in Greater Lizardfish (Saurida tumbil)Abstract Formaldehyde found in the fish has been occurred naturally through the work of trimethylamine-N-Oxide demethylase (TMAO)ase enzyme that broke TMAO into formaldehyde and dimethylamine. Formaldehyde found in fish has been occurred during storage. This reserch was aimed to determine the TMAOase activity and its role in dimethylamine and formaldehyde formation in Greater lizardfish (Saurida tumbil), which stored at chilling temperature. Observations were made every 2 days for 16 days of storage. Analysis performed in formaldehyde content determination, dimethylamine content determination, and specific activity of TMAOase enzyme. The result of formaldehyde test was increasing during storage, from 0,22±0,00 ppm on day 0 to 7,45±0,46 ppm on day 16. The results of dimethylamine test increased during storage from 0,93±0,50 ppm at day 0 to 27,26±1,8 ppm on day 16. The results of TMAOase specific activity in Greater lizardfish increased during storage from 1,15 U/mg on day 0, to 16,47 U/mg on day 16.

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Section: Pendahuluanunclassified

Section: Hasil Dan Pembahasan Kadar Formaldehid Ikan Beloso Selama Peunclassified

Section: Kadar Dimetilamin Kadar Dimetilamin Ikan Beloso Selama Penyiunclassified

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Activity of Trimethylamine-N-Oxide Demethylase (TMAOase) in the Forming of Natural Formaldehyde in Lizardfish (Saurida tumbil)

2017

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Bombay duck (Harpodon nehereus) natural formaldehyde levels and changes during frozen storage

Ariyani

Barokah²,

Wibowo

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Demersal fish, including the Bombay duck (Harpodon nehereus), have relatively high levels of TMAO and hence have the potential for high natural formaldehyde content. This study aimed to determine the natural formaldehyde content of bombay duck and the pattern of the change during frozen storage. Approximately 15 kg bombay duck of 38 – 45g each caught by one-day fishing in Juwata Waters, Tarakan, North Kalimantan Indonesia, was transported in ice to the Laboratory of Marine Biotechnology, Research Center for Marine and Fisheries Product Processing and Biotechnology, Jakarta. Upon arrival, they were split into three groups as replicates and stored frozen for five months. Every month, the levels of formaldehyde, TVB, and sensory properties were monitored, and proximate analysis was performed before storage. The results showed that natural formaldehyde was detected in Bombay duck flesh before storage at a relatively high level of 12.58 + 0.35 ppm, increased to 17.3 ppm after one month of storage, and then decreased afterward. In contrast, TVB levels were low, 12.66 + 2, 54 mg N% before storage, then increased during storage. Based on sensory evaluation, all attributes of the bombay duck were deemed in good condition, with scores ranging from 7-8, except for the eyes, which received a score of less than 6.

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Characterization of Trimethylamin-N-Oxide Demethylase (TMAOase) of Lizardfish from Indonesian Waters

Nurhayati

Nurilmala

Nuraini

2019

IOP Conf. Ser.: Earth Environ. Sci.

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Formation of formaldehyde in fish can occur naturally through the breakdown of trimethylamine oxide (TMAO) become formaldehyde and the dimethylamine by-product in the fish during the postmortem phase by trimethylamine-N-oxide demethylase (TMAOase). The objective of this study was to characterize TMAOase which isolated from Saurida tumbil. The study was carried out by homogenizing of fish muscle using Tris-acetate buffer (pH 7.0) and centrifuged 10,000xg for 30 minutes. The supernatant was then added 1 M NaCl up to pH 4.5 and centrifuged at again. The supernatant was neutralized and heated at 80°C for 20 minutes, then centrifuged at 10,000xg for 30 minutes and then characterized by temperature, pH, cofactor effect on enzyme activity, and molecular weight. The same supernatant and then was dialyzed for 2 hours and determined its enzyme activity, protein concentration, and molecular weight. The result showed that TMAOase enzyme had optimum activity at pH 7 and temperature at 50°C and cofactor combination of cysteine, ascorbate, and FeCl2 could increase TMAOase activity. The dialysate had yield 3.71%, 1.78 fold purification, and molecular weight 20 kDa.

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Pembentukan Formaldehid Alami pada Beberapa Jenis Ikan Laut selama Penyimpanan dalam Es Curai

Cited by 14 publications

References 7 publications

Activity of Trimethylamine-N-Oxide Demethylase (TMAOase) in the Forming of Natural Formaldehyde in Lizardfish (Saurida tumbil)

Activity of Trimethylamine-N-Oxide Demethylase (TMAOase) in the Forming of Natural Formaldehyde in Lizardfish (Saurida tumbil)

Bombay duck (Harpodon nehereus) natural formaldehyde levels and changes during frozen storage

Characterization of Trimethylamin-N-Oxide Demethylase (TMAOase) of Lizardfish from Indonesian Waters

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